Function
Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.
Sequence
MQPGEGYHYDSGPNNAVHPHQLPAGSRLNQYLANNNRQGPSFGPGTPINVNAPVFVPKHQQPQPAQVAAPPPMVNQFAQLSIHDVPHQMIPFGQINGPPTFGPGQHMNHRASHHHQSPQMAQQPPTLQQSAYDRYQLENRGGTTYFYTEPTEAGPDDEQYTEAEAPDGSILVNTPGAFGYNAPLPISHMARFRGKANANLQTQFISPEIRMELINRQLAYDTKADSAIIGDIPHSVEHFSNLVPLEIAGIQSQTTYKAFSCRDGNYYCLRRIHGNRIQHPGKQTHLVEQWKKLVHGNVVPLREVLINCRAFDDSSLIFAYDYYPLAGTLMEKHFDTKSGTFFDPNNGFRISSPMNVSMPISGTGAHETLIWSYIIQIAAALRAIHSSGLACRTLDLNKIITYGNKIMISFCGIQDVLDPDPTTIQQQQNEDLNMFGNLIVALATGRANGWRKDLYQQLKKFIEDTYSMDLRNVIGFLHNNSTRKTINEIMPMIGGRFFTVMENMQAKTDVLEAELSREMENGRLFRLVAKMNTVLERVEHGTDDAWSETGDRFMLKLFRDYVFHQVTDQGKAWLDMAHIVQCLNKLDCGSQEKIEMVSRSGDTQIIIDYATLKRCLDKSFRDLLGTNMMLHR