Protein
PAN2-PAN3 deadenylation complex catalytic subunit pan2
Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
Function
Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein pab1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by xrn1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails.
Similarity
Belongs to the peptidase C19 family. PAN2 subfamily.
Sequence
MEADWDELSRIPVPPPSPHGLPTVATTIAFDDVSELLWAGNEFGRITSFYGPELQRYTSVRAHPVSDGPVRQILFHERGVISLSPKSVHMITRRGLTQWHISHEEMTDLYCMSFTAQLNKIIVAGCQRAMFTIDIDKGIIVDKLHTDHNYTMMKKSRYLCAATDTGSVNALSLTDFRVVKSWKSHGTAINDMDARNDLLVTCGFSVRHLGSPIVDPLANVYDLKTLSPLPPIPFHAGAAYVRMHPKLSTTSFVASQTGQLQVVDLMNPNAINLRQANVSFMLGIDISPSGEALAINDAECSIHLWGSPSKIHFNEMSKEVEFADVVPRPPPLDWSPDTPLNMIGMPYYRERLLSAWPSHLVFEVGSPPAPIDQSLIPYLRPAEMGHYAPNPKKTRRYQVENTRALTTAEPALIAPKFLSEKAREQSKFKSEASVSDTAEALAGAKLNGEAEDDPLLKYSNVEIKYSRFGVDDFDFSFSGLETHIANSFTNALLQLLKFIPLVRNLALHHAASTCIYETCLLCEMGYLFDMLEKANGQNCQATNLLKTFSSYREASNLGLLEENLTTKSLSSAIQSVNRFFLNQIAHDFRMIVPSSDDLDHRLSTIASESIRCMFCQNEIVRPGNSLANELIYPTIDIKQARRNPAFRFSSILRASIERETQNRGWCNYCRRYQQVTIRKSIHRMPLVLILNAALSNAICRRLWSIPGWLPEEVGIAVDNGQVMCFEGDELKTQLQNKLPNLVVYELVGLVSEIDIPEHQKPHLVSFINVSISSREPEAKNRWHLFNDFLVTEVDKDEALRFNQPWKSPCILAYQVKDARHAVDDSWKNVLDTTLLFRDWSLNGGRAVETRQTLTEEEKPTPGTPVALDTEFVDLEKAEIDVKADGSQEIVRPSKSGLARVSVLRGSGVHEGVPFIDDYITIKEPIVDYVTQYSGIKPGDLDPRTSQHNLVPLKVAYKKLWLLLNLGCVFVGHGLASDFRKINIQVPKSQTVDTQYLYFHPGKNRRLSLRYLAWAVFKEYIQEEPADNNQGHDSIEDARMALRLWKKFQEYEDAGIVNQILEEIFREGSKLGFRPPPRNGVATVLSRPGTAVTMQNSSGRNTPSTPDVGGAATATATTSAPATPRQAFRRSIALTPSNGTFSGPGAGDFFGGSPLK