Protein
Probable peptidyl-glycine alpha-amidating monooxygenase pamn-1
Organism
Caenorhabditis elegans
Function
Probable bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
Similarity
In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
Sequence
MNDRISINLIYLVLTFCCVSAATVRTAKNDDIQKFTIQMIGYSPQKTDDYVAVSIEATPGYVVAFEPMAHADRVHHMLLYGCTMPASEQGFWRGMETCGWGGGSYILYAWARNAPNLVLPKDVAFSVGHEQDGIKYFVLQVHYAQPFAGEVHDFSGVTMHISQKKPMNLAAVMLFVSGTPIPPQLPAFQNNITCMFESSTPIHPFAFRTHTHAMGRLVSAFFKHDGHWTKIGKRNPQWPQLFEGIPSKLMIGSGDQMSASCRFDSMDKNRTVNMGAMGVDEMCNFYMMFHYDAKLDNPYPQGAICAKDYPSKMIDYPKDGFELLPSRPELEHHAHQSKVPFGIVQEAIHENLGGVKLGQVAGLAFNNEQQLLVFQRAGRVWDASTFDNYNILLDKKPIADPVILVISYSGNQTKLERKLGGGQFYLPHGIYVDKDGFVYTTDVGSHTVAKWKIEGNELKNIWTSGELLMPGSDQHHYCKPTGITRVEDQLYVTDGYCNSRVVVLDLNGKRIRQFGLPGEDAGQFNLPHDIVSDSAGRLLVTDRENGRVQHMTTQGHVIEEFKSTMFTNIYSAASHEDYVFMVPGRPIMGHETEGIAVFVGRSGTGLIEYAFGPTTKGKREQMGPQFGQPHCLRVCPDGGHIFVGDIAEGKARLWQFKIRHDQN