Protein
Pup--protein ligase 1
Organism
Rhodococcus erythropolis
Function
Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
Similarity
Belongs to the Pup ligase/Pup deamidase family. Pup-conjugating enzyme subfamily.
Sequence
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATAECDNLIQLVNHDRAGERVLEELLIDAEQRLAEEGIGGDIYLFKNNTDSAGNSYGCHENFLVARAGEFSRISDVLLPFLVTRQLICGAGKVLQTPKAATFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSESTTMLKVGTAALVLEMIEAGVSFRDFALDNPIRAIREVSHDVTGRRPVRLAGGRQASALDIQREYHARAVEHLQNREPDPQVTQVVELWGRMLDAVETQDFAKVDTEIDWVIKRKLFQRYQDRHGFELADPKIAQLDLAYHDIKRGRGVFDVLQRKGLVKRITEDETIEAAVDTPPQTTRAKLRGEFITAAQEAGRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDERVERLIASM