Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
Organism
Caenorhabditis elegans
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the SWP1 family.
Sequence
MKLLLVLLTIASVALAAVDDVAVNNFKVGILAKDQQPSDENLKTVALFSKLPNELKADASQRLYVSFTVAKKSDNAKVKPHQVFLRFVAQNGEDVVVVVNPDANGNYVYDNVLRTAAKSFRNLSGQFKISLLVGDVTIKNPINWQFANIDAALPVAYEPTPKSQQVHFEPLNEISHIFRQPEKRPSALISDLFTIICLSPLLILVVLWSQVGINFQNAPASPWVPIFHVGLIGIFGIYFMFWVQFDMFVTLKYLAVLGFLTFVAGNRVLRAISESKQKSE