nlsA

Nonribosomal peptide synthase nlsA

6077 amino acids

Nonribosomal peptide synthase involved in the synthesis of nidulanin A and derived compounds (PubMed:23248299). Nidulanin A is a tetracyclopeptide with the sequence L-Phe-L-Kyn-L-Val-D-Val and an isoprene unit N-linked to the amino group of L-kynurenine (PubMed:23248299). The NRPS nlsA is responsible of the synthesis of the cyclopeptide and the prenyltransferase nptA adds the isoprene unit on the L-kynurenine residue of nidulanin A (PubMed:23248299). Further modifications lead to additional oxygenated related compounds (PubMed:23248299).

Belongs to the NRP synthase family.

677.235 kDa

MAQQAQCLLPSFGTISDGPKRPVSITTKTTPSQSAKLLSAYKNDSLDPLLKTAWGLLLYRYTGLQDVCFGYKHDDAGALVSQTSDAGRLLTFRLTINEHDTIKTILEKSGGGYGCQTDIGVSGSSNANNDNYSSFNTVVMVRVCGDSTKEETFVRPVFQSILPEECRARLHVKVLQEDICIFLEWWNTDISTAQMESVARYFEHILNQVLYSDDTVVANADCFLEQDWARICKFNSVIPETYDRCIHDVISEQVRLHPQREAVCAWDGSFTYGELDVLASELSYRLKGYGVRPETFVALCFDKSKWNIVAMLGVLKAGGAFVPLDPTHPTPRLRSLVDSVNVNIMLCSRNRAEHLSKVVNNLIPLDEQSFGKISFPPRGYLRQEVKSNNAAYLIFTSGSTGKPKGTLLEHRAFVSCVFAYAPLIHGGCVCVPSEEERLNDIVQAINRMNVNFICLTPSFARFVNPSSIPQVNTALLVGEAMSRTDLEAWSHIKLLNGYGPTEAAVCAAINSTMDINSDCRDIGLATGTHFWVVKPNNHDQLVPVGCPGELLLEGPTLARCYINNPEKTDEVFIYNPTWARHDPKRGDRRFYKTGDLVRYNSDLGSLTFLGRKDSQIKLHGQRIELGEIEHNISTLPLVKHGMAFLCESGPAKGRLMAVVSLNGELSSNTVPFKLLPPAERTYAVTELRQQLSKRLPTYMIPAVWLCVEALPLLPSGKLNRREIISWATNKTDDFQGGASESSGVETPKAAHSEVTVEDRLKSIWSRVLAIPRDRISLDESFLALGGDSIAAITCMGYCKKQGMGVTVQDVLQSKSIRDLVTRVQEIKHLVKYQEETEEPFGLSPIQKLHFMIRKEGQGYFNQSVRTRLSRRLSADDLRHAIQVIIERHSMLRVRLIKDTLVGNLRQRITRDIDSSYRLRVHNINHQAQMESAISVSQSCINAFQGPIMAVDIFYTEDDCFLSMVAHHLAIDIVSWRIILEDLEDILLRSEDKTIYTSSLPFSTWCHLQDERTQTFGSYLEDLPIPDAAYWGVENRVATYGDAICETFELGLDDSKSILMECHKSLATEPVDILLASLLHAFGQTFRDRSLPAIYNEGHGREAWDSSIDISRTVGWFTTVFPIFIREQIPDDPVETVVLVKDIRRSVSDNGRQRFASLMSASTKDEKREFLCPMEISFNYVGQHRDLQRQDGLFQLMNQMAGETGQGGGASDFGKETPRFGLFEISALAVNGRLRFIFSFSKYMRHQKRIRAWIASCGDVLRSLGKRLQTHAKRPTLSDFPMLSLTYPDIESMLAKTLPSLGVSSPELIEDIYPCSRMQQGILLARSRDSSLYAVHDTYEVRGFNGKPDVARLAEAWRMVVSRHAMLRTLFVENLTSRDLFSQLVLRNCEPSILYLSCPTDDDVVSTFNSQRPEIYNEYQPHHRLTFCETASGRVFFRLELSHAAMDGVSISVILRDLQLAYDGKLDQNKPLFKNYIQYLRNTPQDASIVYWKNYLADVKPCLFPTLTDGKIIAQKQLKVLRPKFNLFNDLQTACEERRLTLSSAFTAAWGLTLSLFCGSNDVCFSYMTSLRDALVDDIESVVGPVINLLACRVKISEGDTLRDIMQKVQNDCMEQLAYNTLSLIDIVHELRLSEQALINTGISYQRVTKMQMHHTTGINLSRVCAIQDPAEYPLFVNVVASDKAAEIEVNYWTDTLSDEQAESVSSTFFKCLENIVRHLKEQVGQLEVLSDWNKQRIRKWNKQLPEEVDMLVQDIIQEKMASQPDKPAIIAWDGTLTYAELEYLSSCFAAYLQQLGVRRGTLMPIYVGKSVWQIVAILAVFKTGAICVPRDEAQLGDSVDKWLVDHGAHIVVTLPSLAGSLERQFPVVVPINKSLFEFLPSSSQENLPQVYPHDDSFIAFDSSDPHESSAVLDQRAIIARAASFASTINSNSGTKTFQYAPCTSDMFLQEVMGTFMSGGCLCIPRSDSLSQLSRSINETSANLICLTPLVASFIRPSDVPSIQVLVLFGEQSARNVRNIWSEKVQLYTFYGRTECSSTCIQVSGLDDLDTQSSIGTSVGCCSWVVDPQDFTRLVPVGCIGELVIEGPSVSRGYFCHEKQKKERFTEQDRGLMEPAKRPYTLFPGSRRKMFRTGYLVRYNADGTLVYLGEKVDSMDQTLQMIAFKIEQLLDVQGSAGYRCVAEILDLRIEEYPEPCIAVFILSTEKQQSNTIKQSTVIARKTNNSHMLMAKLHASLAASLPASQVPSLYFPVFGLPMTSLGKVNRPLLRKAVKSLSADSLTEYDLKKFGEFWRHQLEKPSLSGQHLLQPFPIQESPALKMVDKGELLVNAKESSNSAEQFLPQATMIPRRVQVNNSTSISGLLDQTASCLVKARPYEKTPLSSIRSLNADTSQASDFDSALSISSMTSQQQSQYLRSLENAERLHSRFSACPIVVFCALEETGVSLEIRYDDRAVYRSQADRLLALFGECLNIFKSTTGLEEKVADLSKRGGNLQIFNDTIDYWKVQLTDIESCLFPDLSPKKGESRLGTETLRLSNASKMQSACKALSINPNILLQTVWALVLRCYTGLEDVCFGYHVSTKKDSVNILPCRFNLNDDLRLQDVMQKRKEDMESASKYQMPLFEILRAIGSENSPIFNTAFRYRKSSSNAAVFNNAVLDPVNEGLNEYLISVNASVSGSSAEISFDYQSTSLSETDIGHIIDCFECILNSILTLLGPSRVIRDVEFFGRQSCQKVSAWNASLPERPKRCAHTIIQDRVIAQPSAPAICSWDENFTYSELDSLTTKLAYHLMDWGVGPEVFVGLCFEKSAWAVIAQVAVLKAGGAFASLDPAHPESRLRGLVDDIAAPIVLCSTRYLDKSSRICMAALAVSHYTLEQIPDSPATRSLPTLSVENAAYAIFTSGTTGKPKITVLEHAALDVASSCFAKTLGIDSNTRALQFSSYTFDVSILETIITLMTGGCVCTPSDDERMNDLAGAIKRMEANSISCTPSVISTLDPSSVPTLKTIFTGGEKLTEAQIMRWADRRFYNAYGPSEATIIATASLKVNRDGIRLDDDCNSIGTAVCGRAWIVDPYNHHRLLPVGAVGELVLEGYNIARGYLNNDKKTKEVFITLPRWLRDSGLRDVPKPTGRMYRTGDLVRYKSDKNISFISRMDTQVKLNGQRIELEEIEQQCTFISPANTQVAVDIVVPETKTVAKALAAFITIAGHEAQSATPGLGVSSSLLLPLSDSIQRTIGQLHNSLGQVLPQVMIPRLYFPVRYLPLGTTGKLDRKGLRAMVQALPKEQLISYMISNVGSGRAVERAAESTLRDLWAKALEIEPGSISAEDSFFALGGDSFAAMKLVGAARSQNISLSFATIYEHPVLVDMAKCCDDTEKPAERQRADLRPFTLVPGSIPLHDIMEEVSEQCSVTKDSIADIYPCTAVQEGLITLSIKSPGSYVARIPYRLAASIDLQRFKAAWQQVTDEFDILRTRIVHIENTGFLQVVLKKERISWTLETSLDNVTDDTAEGSGALLAKYAIVQLGTDSRYRQCLYKLFIHHLLQRDMQQSDEFWKSYLDGLSCEPFPPKKNKDLSCSGAGSIHRASVDISRKVGTTDTTVPELVRSAWAIVLSVHTGSGDVCFGETLMGRNIDMPGITDVVGPVLTTVPMRIRVDNKLPINQYLRDVRQIITTMIPHQHSGLQRIQKLSGDAALACNFQNLLVIQSDDSQLNDDIWSPVEQDTRGDFFTHPLVVQCQISGPRLLILANYDELVLDDWQTERLIGQFSFVLEQLLSVPRDSLMTVGDIDITGPLDKRDIASWNQRQVTCVNKCVHEIIRENAIMHPQATAICSWDGEITYEEMFQLASSFAAYLVICGVGPETLVPVCLGKSLWTMVTVLSVLLAGGAFVPLDPSHPTSRHKEILEEIEADMILCSPQLRSRYLGSVSTIIPVSEDTIKAYSTVTTSEKANASPTPENMAYAIFTSGSTGRPKGIIIEHRAVCSSVIGFAPVVGLNKESRVFQFASLTFDAAILEVLGTLMLGGCICVPSDDERLNDIPGAMQRMNVSWSFLTPSVACILEPSTVPSLQILTCGGEALSSEVVKKWTGHVKFYGGYGPTETVVFAVVARDFVDHDFTCIGYGVPSTLTWVVQPDDHDRLAPLGAVGELVLEGPALAREYLKNPSKTTDVFINEPAWIKSFPSSLPSPRRIYKTGDLVRYNPDGSIEYLGRKDHQVKLHGQRMELGEIEHRLLASENIRNAVVILPQKGPLRQKLVAVLSLKSLTVESSTIMTGSCELASQKDMLETGYRQIRTSQKSIEEQLPVYMVPQAWAVVKSIPMLVSGKLDRKRICTWLEQIDKSAYDRIMQDYDNVDQVIVEEENKGEREGDATPAIIRDIFAQVLNLPLNKVDPSRSFIYLGGDSISGMAVVSKARKRGLNLPLNRILQAKSIEELAVSCGTKPLPTKNVKESGSLFPLSPIQELFFRSASVLPKALGRFNQSITVRLARRTEPNVLEDAVRAVVQKHAMFRARFSKSSDGTWRQRITDEVDSSYKFCTHPVKNAGNMLSIIADTQSSLDIQRGPVIAADLFDKNGEQILFLVASHICVDVVSWRIVLQELEDFVDTGSIPSDVPLSFKSWCNVQFEESKRLNKSIEIPCQQADLNYWGMSRAPNNYGHVKMDSFALDKQATAFISGHFHEILGTETMEVLLAAVMYSFNRVFPDRDAPTIYNEGHGREPWNYSDPSGTIGWFTTLNPLHVEASSDLLELLKQVKDTRRRISEHSRAFFAHNVLHSDSTDRTHMFSIPLEILFNYLGQLQQLERGGSTFQHYGDVFSAETMDSASDMGPETPRFSLFEITALILKEQLHISFTYNRNMRHQARIQAWMAECKRVLEVELPKFRNVAPQPTLSDYPLLPITYHGLEELTASVLPRLGLESWRQVEDIYPCSPVQEGILFSQLRDPHEYIFNAIFELRQSGNKGSFDLARLKKAWSTVVVRHPVLRTVFIDSCCEEGSFDQVVLKEASDATVLIECDDLDALNKLEAVSLRSNKSLNLYHQLVLCKTSTGRVLMKLEMNHVIIDGGSTSILLRELALAYSNQHPPGPGPLFSEYIKYLREQPTAEALEYWKRRLSDMPPCHLPINASENGARQLGTHLVAFNRFAALQSFCEANSITFANLILAVWAIVLRSHTKSDDVCFGYPSTGRDLPVPGIQDAVGIFINTLCCRVRFDTNQTLKGTVKSVQEDHIASLAYQRSSLAEIQHALGRKGEPLFNTCISIQNRSEDKTEIAGISYEFQKAHDPCEMLGKGKPVTSWVKSHGAESPSDFPDIRNDVEESLQDLVVMMEKTPASSTQTLNTDYRAPNDSEKQLWRLWSITLGLPPHPVKYHDSFFRLGGDSITAMRLVRAARDEGLKLSVADVLKNPVFENMMALINDRKKSIPTTVTEKRADSIEKRVEDKPILTKCESSQDISILRPMSLEFDDTSLRAAISPKVGVFKGGIVDVLPVTDFQALSLTATMFESRWMLNYFYLDGKGSLDIRRLRESFLRVVDAFDILRTVFVCFHGQFYQVVLRKIKPDIFVHETEKGLDEYTNSLQQRDREQSPGQGQQCVQFYVVRKTNSDEHRILVRMSHAQFDGVCLSKIMTAIKMAYEGSPVSPSSFLNYMRLLPGNITPEHYQHWGNLLKGSKMTQIVQRDRPNTFQHIGGFTQQSKVIEIPSTATENVTIATVMQSAWAVTLAKICAQDDVVFGLTVNGRNAVPGAENTIGPCLNFIPIRVTFKDCWTGLDLFRFLQDQQVANMTYESLGFREIVRRCTDWPESTFFTTSVLHQNVDYEGHMQLDNNTYKMGGVGVIDNLTDLTLFSKPVAGQPAQINVALGYSTKGPIHPSFVSTVLDMVCDTAQSLVANPNVALPSPSTIRSLPPQLVEDIPTTGSTDSLLSSLNNHSLSEILVHSDLITRIWQQVLPPKLNTGKPPSSYQLDSSFFGLGGDIVNVAQVVWILEQETGLHIRLEDLLAHSTFLGHMAVLALNMTKRDSAGVDSDAAPAPAYAPVDARASRNVSTSRQQQEGLPLPAANAKSEWSALDRARVLAKKITRLGGLGTRV