Protein
3-hydroxybenzoate 6-hydroxylase
Organism
Polaromonas naphthalenivorans (strain CJ2)
Function
Catalyzes the NAD- or NADP-dependent conversion of 3-hydroxybenzoate to gentisate. The affinity of the enzyme toward NAD is twice as high as for NADP.
Similarity
Belongs to the 3-hydroxybenzoate 6-hydroxylase family.
Sequence
MSDNPADLPVLVAGGGIGGLAAALALVRRGFSVKVLEQAPEIGEIGAGIQLGPNAFHAFDALGIGEKARGRAVYTDEMVMHDAIDGSLVGRIPTGEAFRQRFGNPYAVIHRVDVHLSLLEGAQETGKVEFLTSTRALRIEQDEGSVTVYDQHGNAHKGIALIGADGVKSVVREQFVGDAARVTGHVVYRAVVDKKDFPESLQWNAASIWVGPNCHLVHYPLRGGEQYNVVVTFHSRQPEQWGVTEGSKEEVQSYFQGICPQARQLIDLPKTWKRWATADREPIGQWSFGRVTLLGDAAHPTTQYMAQGACMAMEDGVTLGEALRVNNNDFPKAFELYQRSRVARTARIVLSSREMGRIYHAQGVERLVRNDLWKGRTPERFYDAMEWLYGWNVGNCLAKD