Function
Methylphloroacetophenone synthase; part of the gene cluster that mediates the biosynthesis of usnic acid, a dibenzofuran lichen product possessing a broad spectrum of biological activities (PubMed:26895859). Two genes, mpas and mpao, comprise the usnic acid biosynthetic gene cluster with a single post-PKS enzyme, the methylphloracetophenone oxidase (mpao) (PubMed:26895859). The methylphloroacetophenone synthase (mpas) is a non-reducing polyketide synthase that produces methylphloracetophenone from acetate via a methylated tetraketide intermediate (PubMed:26895859). The methylphloroacetophenone oxidase then carries out the oxidative dimerization of methylphloracetophenone to usnic acid (PubMed:26895859).
Sequence
MALPSLIAFGALAPWPASDRLDQLRNALQHHNSLKPITKAIQELPLLWKALSNQDQSLHSIAGEAAADQLAQWISGAGTAQLVDDKGNVTRMPLTTIAQIAQYVSYLCQYEEPLRHESIIKSAAIGGGIQGFCIGLLSALAVASGKTEDDVGNFAAMSVRLAFCVGAYVDLDRHRNGGDSKASTIAVRWKTPTTLEDIQRLLSRHPDTYIAVVRDIRDVTITVPASVMEHLIEDLSQIGASLRDTGVSGRYHVAIHEGIPQKILETCQAQFSPTINGQPLVRSNTDAHLFSGEDTALLALECILGERADWYSTISTAASALNQISANPFILSIGTDPVPQSVARSFPVVKATMIADRVNGIVEPEIPALAPDPFGSVSQGYPKDAIAIIGMGCRFPGADSIDEYWNLLTEGKSMLSEIPEARFGRGRPARSNSSLRFWGNFLRDIEAFDHGFFKKSPREAVSMDPQQRVLLQVAYEALESSGYFADSSRPEDVGCYIGACATDYDFNVASHPPSAYSAIGTLRSFLSGKLSHYFGWSGPSLVLDTACSSSAVAIHTACTALRTGQCSQALAGGITLMTSPYLYENFAAAHFLSPTGSSKPFSADADGYCRGEGGGLVVLKRLSDALRDNDHILGVIAGSAVNQNDNCVPITVPHTSSQGNLYERVTEQAGVRPSEVTFVEAHGTGTPVGDPIEMESIRRVFGGLHRVAPLIVSSAKGNIGHLEGASGVAALIKALLQMEHHLAPRQASFKTLNPKIPALEPDNLCIPTSNLALSGERLAACINNYGAAGSNAAMIVLEPPRKSVTYHDKSKMSISSRPKIHPIQLAAASLGGLLAYCVALDQYCQRLRFTQDTSEQPQVLSDLAYSLSTRLNQELPFTLTMTVTDLDQLQAQLRQQTVTSNNIKQRSKAPPVVLCFGGQVSDRVALDKCLWQESTLLRSYLDICDNTLRVLGYPGLYPSIFQNEAVTDVVLLHSMIFALQYSCAQAWLESGLKVDALVGHSFGQLTALCVSGILSLRDGLRLVAGRASLMQKHWGPESGKMIAIETDQQTLEELQKVICESNASYNFEIACFNGPTSHVVVSDRCSASELETKLMERAIRHRSLDVPYGFHSRFTEPLLPHLEDLASSLTFHEPKIPLETCTDMGTWTEPTSKLIAAHTREPVFFGKAIQRLQARLGPCTWLEAGSDSSIVNMVRRALGQASATANNFVSLQLNKPNSSKLVVDATVALWDASHRAQFWNFHRLQRRQYDHLRLPPYAWEKSKHWLELDMSAALNSDKTNTPPPTNTAAQVELPAVLIRLKSFDSQGHHFVINPSSEEYQTIVKDLESLGSAVCPSTLYVELASRAVRVAEEDKGNGLLSIKDLRVHSLLGVNVHQTISLDLQRLAQSWRFRITNADGSISGSNPGESFCHAEGTVNLKVADDSLEEEFCRYERLTGHNKIISIADDPRSESLRGNVLYNMLGRVVNYPDWYRGVKSVAALDLRVVAKVTCPVGIPEIVSKESTTQLPILESFIQIASLHANCLHECRGGEVFQFTRADHMQWAPGFDLHGYGDSAEASWDVLAYNSTNAENVVYDIFVHDAVTGRLVLLVLGANFTDIRRPVPISAGLNTSLASEKDIPMLKNANAERAEISLNSQLPAESHSQANLTRPGKDAKTSIYEDICGLLEKLADIPGDQVSGEATFDNLGVDSLMMIEVISELSTLFRVDLPIHELEELTDINSLVDYLHGKGCVGSLYVEDSGNASSLSSSHAISTGASSPPDSSGASAMTTPPETLSLVDYPGSLTTKQESRAAPAISNGTGRQPLDMGPYGIQQVFTRLRFDFEKYAEQTGAKGFWTNVYPQQADLVCAYVVDAYRKLGCDLATLAAGQQLPSMNTLPRHKHLVAQLRNILVESGLLELRGNQVHVRTAKTVDSTPTAIRYEQMLQRHPFGASETKLLNVTGPRLADCLTGQKEPLSLLFGDKHNRDLLADFYANSQMLKAATRLLAEFVSSTFSAAQSGDTLCILEVGAGTGGTTRYLVDVLNRCGIPYEYTFTDISQSLVTQAKRNFASLPQMRFMTFDCDRPAPQELLGKFHIVISTNCIHATSNITTSTTNILPTLRDDGVLCLVEFTRNLYWFDLVFGLLEGWWLFSDGRQHALANEWFWDRSLRAAGFKHVSWTDGNTEEAKTLRLICAFRGEAKEDRNLAAPNGAITKRAGVPMEEVVWKRVGTLDLSADIYFPKTPDPPGKKRPIALLIHGGGHFLFGRKDVPMKHIRTLIERGFLPISTDYRLCPETNLFEGPMTDCCDALKWATETLPTLPLSGPTVRPDPTKVVSLGWSSGGQLAMSLGYTAPVKGIKAPDAIFALYPPSDMESNHWHQPCYPLAAEEEPTEILDILAGVRESPIVEYAPVSEKRTMALSLTLKDDRASIILHMCWKSQTVPILVHGLPYKKNLPDTDKTDWKYRPPASAEQVQAISPLWQIRQGNYKTPTFIVHGNGDDWLPLSMSERTVEELKRRGIPASLAVPEQCGHAFDLFPVGDPLGVGWTSLEQGYDFICRQLGMS