Protein
tRNA-specific 2-thiouridylase MnmA
Organism
Photorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)
Function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs.
Similarity
Belongs to the MnmA/TRMU family.
Sequence
MSDNSQKKVIVGMSGGVDSSVSAYLLQQQGYQVAGLFMKNWEEDDDEEYCSAATDLADAQSVCDKLGIELHTVNFAAEYWDNVFEHFLAEYRAGRTPNPDILCNKEIKFKAFLEFAAEDLNADYIATGHYVRRRDINGKSQLLRGLDNNKDQSYFLYTLSHQQIAQSLFPVGELEKPEVRRIAEKIGLVTAKKKDSTGICFIGERKFRDFLGRYLPAKPGPIMTVDGESLGEHQGLMYHTLGQRKGLGIGGTKEGSEEPWYVIDKDVQNNILIVAQGHEHPRLMSTGLIAQQLYWVDRQTLTEKIHCVVKTRYRQQDIPCSVTPISEDKIEVHFANPVAAVTPGQSAVFYQGEVCLGGGVIEQRLQE