Protein
Citrate/2-methylcitrate synthase
Organism
Bacillus subtilis (strain 168)
Function
Involved in both the tricarboxylic acid (TCA) and methylcitric acid cycles (PubMed:28956599). Has both 2-methylcitrate synthase and citrate synthase activities. Catalyzes the condensation of propionyl-CoA and oxaloacetate to yield 2-methylcitrate (2-MC) and CoA, and the condensation of acetyl-CoA and oxaloacetate to yield citrate and CoA (PubMed:8759838, PubMed:28956599). Has 2.3-fold higher activity as a 2-methylcitrate synthase (PubMed:28956599). Catalyzes the formation of either (2S,3R)- or (2R,3S)-2-methylcitrate (PubMed:28956599).
Similarity
Belongs to the citrate synthase family.
Sequence
MEEKQHYSPGLDGVIAAETHISYLDTQSSQILIRGYDLIELSETKSYLELVHLLLEGRLPEESEMETLERKINSASSLPADHLRLLELLPEDTHPMDGLRTGLSALAGYDRQIDDRSPSANKERAYQLLGKMPALTAASYRIINKKEPILPLQTLSYSANFLYMMTGKLPSSLEEQIFDRSLVLYSEHEMPNSTFAARVIASTHSDLYGALTGAVASLKGNLHGGANEAVMYLLLEAKTTSDFEQLLQTKLKRKEKIMGFGHRVYMKKMDPRALMMKEALQQLCDKAGDHRLYEMCEAGERLMEKEKGLYPNLDYYAAPVYWMLGIPIPLYTPIFFSARTSGLCAHVIEQHANNRLFRPRVSYMGPRYQTKS