mlfA

Malformin synthetase mlfA

5112 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide. MlfA probably acts iteratively on one amino acid and possesses multiple amino acid specificities since it is involved in the biosynthesis of multiple malformins, including malformin C and malformin A2. Malformin C corresponds to a cyclo[D-Cys-D-Cys-Val-D-Leu-Val] pentapeptide whereas malformin A2 corresponds to a cyclo[D-Cys-D-Cys-Val-D-Leu-Ile] pentapeptide (PubMed:30560908). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

563.09 kDa

MSRFSCIFPTLTDGYVPNPDHTRAAGRRTYTIDLSGWNGSSGQAESYILAAWGLVLSSYVGTDEVAFYVVPTTGPDTTALAELKVEGDMSRLSLTDAAEQLLHPKHVGAGQISGETANTIITFANDIESLFVTQTEESFLLLHVHRDEKGHISLTLTYYLSLLTDAQAANVCTAMSQALAVVTTDHPERLIKDLNLMSPTHIDHIWKFNANVPGPWEECFHDVVERHAANRPHSLAVDAWDMKLTYAELVREAHLLAAYLQQRGVRPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPNLPAGRLDAILEVIEAPFVVTWSKYEAFWSERLSTLPIDNYPKPSGDATVESLGKPDDLFYVIFTSGSTGRPKGCMLSHSNWLNGALRNAPSWKYGPESRVLQMLSHTFDMSLLEICTSLGSGACVCVPCTEEIETSVSDAINRWQVNHVIMTPSLARALKPDDVPGLKTMCLGGEAFPKEIVTMWSERINLWQFYGPSECSINSSSRPITRPDADPLNIGPPNSAACWVVDAQDYNKLVPVGAIGELLVSGPIVGMGYLKNPVKTAEAFLDQVGFVSKDDPQFGGFRFYRTGDHVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLEDGVQYAIAMSPQTGRCKNNLIAILTVKGTNCSNQRNAADEIPLLDRRDPIIQQTVKKLRSQLQHALPRYMVPTIWAFVGRMPMSASGKIDRVQLRNWVQEMSQETFDAITGRSFEAEDHVLGLSRLEQEIQLAWAEALGLSAAEVGLQQPFVALGGDSIKALDAVARCRTRQIKISMVHILSCEGVREAASLAEVQETPAQQVAEMAVDYSDLWTRLSTEYDLGKLGVTQVEDVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGCLPTVDRIQQAWASLIERHPSLRTIFVDDLSPDAIYHSVVLRSVPLELTMREVPRDLSPEDALAMFTDELVPFRPNAPLHRMLLLTCRGRVPYFMLEISHVIMDGYALSVFRREFIQACSSTGPLPRGPDYRMFANYHRTRQTDDSAKYWTNYLSDCVPCHIPTHAVIAPTDAPPEWPRNLQRRDFSFNNSAAFLQRCKERQVTLACAIRAAWALVLRAYTQSQDVCFGYVSSGRNVPVPEVETIFGLCLSMQVCRARLSESSTIASLARKIQEDYVASLPFQHYPLAEAQRGLKQTHGQGLFNTAISMEWVPPTAEDGDALLDLEEIREQDDPTEYDVAISVDIHEGHIKLGFLYWPNLTEFEIAHLAEALQGAMNCFAYQPDDALDSLTLLQASDVCSTLADGPTLLPLEAVRVNMLSMIDRRVTRQPDTPAIEGWDGSLSYKQLHEQSCWVARNLLHQGLQLGDRVLVCADRSSRTVATILGLVRAGCVLVLSNPTDPDKRLHWLAEKCNAALIIADPAYEKRFATAGSRVLLTTAVCSPAAWDYEFPALDEHDLISILFTSGSTGTPKGILMDHGALATSVLLGHGRTLRFSRQTRMLHFASLTFDAALAEIFTTLAHGGCICVPTEEDRLSDVPGCISRFAVNTAMLTPSVGRLLDPTALPMLKSLIMVGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMTPTDEPTNLGHSVAGVRLWVTEAEDPNRLAPLGAVGELIVEGRLVTRGYLDDGERTQQAFLPSLPWLPSQHALYRTGDLVRYADDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQVVVEMVIPEGKTRAQASLVAFVSGLTAADVESSSACNSEESMTISQVVLPKSTIQTLEEALPRHMIPSVYYALETIPLSVNGKADRRRLREMGASLLASSAANKSTADGKKEPAKWTAASELERTLLELWATTLGLEVEAIHGDDSFFELGGDSVSAMKLVATARDKFKLSLSVPQMFRYPTIRQLAAELGESPRSSTSSASSSTEDEFTISTPDDSSTNDGVDDDFLQLATAQLAQLAQEKGKKVDIAALLKQLQGGSSSSKTPSVSSSSSSSSSRKKKSSKAAFPVEAPGPVPEPFSLLNGDADVVEQVRAHAAEQCKIPHEDIEDIYPATALQEGMMALMARTPGVYTTALTCELSSQIDLARLHSAWDKAAEAHPILRTRIIMTDDNTAVQVVQRAKGLPWDRYTLQDGESIPNLTSDMTLGSPLLRLAEIHRHSKPPMLLVAIHHALYDGWSMPLLKQAVEDAYHGQTLQPQPFTPFINYLKEGKRAAQDFWTAHLDGFAGGVFPNLPSIDHHIQPSERRSRSLTIPTAVSRNQYTMATKIQAAWAVTVSRYAEDEDIVFGTVSTGRSAPVPAIDRMVGPTITTVPVRISLGDQAQRVSPLLQRVQEDGWNRMDHEHLGLQYIGRLSESAAAACRLQTLLVIQPREESHANSGATLLSGLQDSAELEGVDTYPLMLVCEPDGASLHLTAVFDPLVLDRTILERMLAHWELVLTQLWTEPDMAVVDLDAVSYSDKQTLVQWNVGEKIADGCAHDAVHEWSIRTPHAPAVCAWDGEWTYEELDKCSSLLASQILEHGVTSGDFVALYHEKSRWVAAGILAVFKAGGILVTLDPAHPKDRIRDILDQTQPRLILTSQSLLGEARELDTPVLCLQFAASQPVPERCSSPPTVSPTQAAYAPFTSGSTGRPKGVPLEHRGLAASTASVSRACLLRPASRVLHFASFAFDASIMEPLVAWHAGGCLCIPDETARQTDLARCIRDFDVTWAFLTPSCLRLITPDDVQCLEALALGGESMTPEDISIWCPRLNQMVQLYGPAECCFVAALTEVTKPSENRLIGRPNACRCWVVNPKSPERLAPLGAIGELMVEGITVGQGYINNPERTTQSFIQPPTWLQALYPDEEQPRHLYRTGDLVRYAGEDGKLTFIGRRDGQVKLHGQRIELADVEAHLRPLIPAKHNIVVEMISSVDNQHPLLAAFVEEPSPSQGPQEQHIGLIHPSEAQYALNVKTIDGALSRTVPQHMIPSMYLHISRLPLSSSGKLNRRQLREMVAQLPRQKLNEYAAGSCEMASQRPTTAKECEMQAIWARVLAADPDTIGLNDDFFRIGGDSISGMQIATKCNAAGMHITSADLFRHRTIAQLLFHLRNAKKRGDAISLPAEPVEEWVDLAPIQQLFFEIAPEGSSHFNQSLLLRTSRHISVEELTAGLDILVKRHSMLRASFRRSESGHWSQQVRSLDSPAGTFYRLATHNGITRESLPSVFTTSQTSLSIQEGPLLAIDLVELTDGSQLLYLVAHHLVIDLVSWRILHGELEQYLQTGSLEPVTESVPFLTWSRAQAEYSAKHLTPVSALPRFQEAHDEFDGARYWGIPPESNTFGQTSTFRFTLDQTATDTLFGTGNNVLGTRPVEILQAALWYSFTQSLTDRPYPSIYVEGHGREPWADSIDISGTVGWFTTMSPLVFAPWDSLSRTSMEDFLDALSYIKDQRRRVPANGWAYFTSRYLNDEGKVAYGRMKPVVEILFNYMGQYQEMTREDAILQLAGDDIQSGTGAADIADDVPRFSLIDVGAFTANGCLSFEFIFPECIQRDARLKLWVENCERMLLSAAKLLSNEGPRKTLSDFPLMPELTYEQLSQCFEHTLPSMGLSASDVVNIYPCSSVQQGMLLAQLRDPQAYQQRFKFHVKSDERRLTLEQVKDAWREVINRHDILRTLLLPVSDHGHLDQVVMAPGSLQHLVRIDATDVNLTQGIPHSINITRDSSGTVVCEWNVSHVLVDAMSVAVIQLEVSQSLDGLLGQHEKPGQYADYIQWVSRRDKTETQAYWQRYLEGVEPCLFPKLTSTSDNVKPEGTISAVRATWDRDARMDELCQKHGITLTNLFHIVWALVLGAYVGTDDVCFGYTTLGRDVPVDGVEMMVGPLVNVLATTVQLRDEDSILDALRTHQAHLTNSLQHQHHALADVYASIGLVGSQLFNTIVSLQDVSHFDAPDEQSTRLEVQPANDASEVCHYLLVYKCFIFFDVNFRKYDVALNIGVGSSSIQLVCSYRTLSLSAEHADTLLRTASHVLSEILRDPTQRIQEIEIISPECKEQLVKWNPTVPAPSDEYIHEKIQGQCRLHSSRQAVCAWDGIFTYAELDDLSSRLAVRLTRMGATSEHIIPIYSPRSRWTVIAILGVLKTGAAFTLLEVSHPMNRLQEVCKQIDASVIIAPASHATSAASLAPILVVLDNITSMTPGQSESSPAVRMPPASEALAYLIFTSGSTGNPKGVMVTHQNLCSNASIMTTSVNMTADSRVLQFASYAFDASLWEMLGALFAGACLVIPSESESKEDLAGCIDRMAVTWAFLTPSVARILKPERVPQLRVLALGGEPIAVSDLDTWRTHAQVVCAYGPSETAILASTTSPSTIPTVGKDIGMPTTCSLWIVDKRDYQKLAPLGATGELLIEGPNVSKGYLGDPEKTNEAFPVAPRWLSQLRQSPTRVYRTGDLVRFDQSTGTLRFVGRKDNQIKFHGQRIELGDIENHAQQALPNASTVIVDLISPGESQQSYLVAFVYQPDTSTQTADAIDPILLPPSESFRTDALAAQKHMHDRLPHYMVPTAFLPLNCLPLSNTGKADRKRLRHCALALSGSELNAYRATATAKRMPSTEAECIMQQLIATVLGRDASEIGMDDSFFHLGGDSVQAMRLVSEGRQQGLTLSLRTIFDSPRLDDLARHNSLVQDDQPAAASPATMHDTFSLIDKLVSTYPIDKAAVVDILPTTSFQRHWLDAQLKSYIVVDIPGRISRDRLFTAMQRVVDAHPILRTSFVPHDNTAVQVILRTAFAITDADLSTTTVEDLCRQDANAAIAPGAPYLRVILATGEFGYKLIMRLSHAQYDAVSLSLLMNDLGHAYENDTHELPSSYSPSFTDYITYQQRQKVDSTATTFWRHLLQDVPLTSLDLQPSKPSTSNGTPITRTRDINIATFPQLPNGITLATAVKAAWSIVLAQKTNSPAVIFGQVVHGRGIPLPGVEGIVGPCANITPVVARLSPQTTGLELLQALQDQHRSGLSYEAVDLDDALAYTKGWQAGSPRVQTIVQHQNNVMTDGMGLSLGEVKCGVDVRAVDHVPREVWVYSSVDENKPGMLEVKIMSSTLVLSEEVAEELMDLVVEKVVALLRDPRGVCV

mlfA

Malformin synthetase mlfA

5104 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

561.642 kDa

MSRFSCIFPTLTDGYVPNPDHTRAAGRRTYTIDLSGWKAPGSEKESHILAAWALVLSSYVGTDEVAFYVVPVTGPDTTALAELKVEGDMSRQSLTCAAEQLLHPALVGAGQVSRETANTIITFANDIESLFVTQTEESFLSLQVYRNEQGHISLSLVYYLSLLTDAQAANVGTAMAQALAEVGTCDNDRLIKDLNLMSPAHLEHIWRFNANVPGIWEECFHDVIERHAVNRPHSLAVDAWDTKLTYADLVREARLLAAYLQQRGVRPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPNLPAGRLDAILDVIEAPFVVTWTKYESFWAERLPTLPIDNYPKPAADATVEALGKPEDLFYVIFTSGSTGRPKGCMLSHSNWLNGALRNAPSWKYGPQSRVLQMLSHTFDMSLLEICTSLGSGACVCVPRTEEIETSISDAINRWQVNHVIMTPSLARALRPDDVPGLKTMCLGGEAFPKEIVTMWSERINLWQFYGPSECSINSSSRPITRPDADPLNIGPPNSAACWVVDVQDYNKLVPVGAIGELLVSGPIVGMGYLKNPVKTAEAFLDEVEFVAKDDPQFGGFRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLEAGVQYAIAMAPQAGLCKNNLLAILTVKGTSTGNQDTAADEIPLLDRRDPIVQETVKKLRSQLQHALPRYMVPTIWAFVGRLPMSASGKIDRVQLRDWVQKMSQETFDAITGRSLEAEGHVLGLSRLEQEIQLAWAEALGLSAAEVGLQQPFVALGGDSIKALDAVARCRACQIKISMVHILSCEGVREAASLAEVQETPAQQVAEMAVDYSNLWTRLSNDYDLGKLGVTRVDEVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGCLPTVERIQQAWASLVERHPSLRTVFVDDLSPEAIYHSIVLRSVPMELRMREVPRDLSAEAALAMFTEELVPFRANAPLHRMLLLTCRGRVPYLMLEISHVIMDGYALSVFRREFIRACSSSPPLPRGPDYRMFANYHRTRQTDESTGYWTNYLADCVPCHIPTHAVSAPSDAPPEWPRTLQRRDFGVDNSAAFLQRCKERQVTLACAIRAAWALVLRAYTQSKDVCFGYVSSGRNVPVPEVEAIFGLCLSMQVCRARLSEASTIASLARRIQEDYVASLPFQHYPLAEAQRGLKQTHGQGLFNTAISMEWVPPSVEDEDALLDLEEIREQDDPTEYDIAISVDVHEGHIKLGFLYWPNLTDFEITHLAEALRGAMNCFAFQPDGALNTLSLLQASDVCSALADGPTLLPLEAVRGNAVSMIDRWVTRQPEGAAIDGWDGSLSYKELHEQSSWVARNLLHQGVQLGDRVLVCADRSSRTVAIVLGIVRAGCVLVLSNPTDPEKRLQWLAKKCDAALVVADPTYEERFATSGSRILSTTSVCAPAAWDYEFPALDEHDLISILFTSGSTGTPKGILMEHGALATSVLLGHGRTLRFSRHTRMLHFASLTFDAALAEIFTTLAHGGCICVPCEEDRLSDVPGCISSFAVNTAMLTPSVGRLLDPGALPTLKTLIMVGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMKPTDEPVNLGFAVAGVRLWVTEAENPNRLAPLGAVGELIVEGRLVTRGYLDDPARTQEAFLPTLPWLPSQHALYRTGDLVRYAEDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQIVVEMVVPAGKMRAQASLVAFVSGLTAADVESSSACNFGGTIPISQIVLSKSTFQALGEVLPRHMIPSVYYALDTIPLSLNGKADRRRLREMGSALLASSAAHKNTIEGMRESVKWTPASELERTLLELWAATLALDAETIHGDDSFFELGGDSVSAMKLVATARDKFKLSLSVPQMFRYPTIRQLAAEFGGPAGQSASSASSTTEERFTFSTPDDSSTNDGVDDDFLQLATAQLAQLAQEKGKKVDIAALLKQLQGGSFTNKTPSVSSSSSSSSSSEKKKKAAKVDSQAEAAAPVPVQFSLLDGGAEFLEKVREQAVEQCKVPHEDIEDIYPATPLQEGMMALTARTPGVYTTTLTCDLSEEVDLARLHYAWGKAAEAHPILRTRLILTDNNTAVQVVQRAEELPWDTYSLHDGDVLPDLTSNMTFGSPLLRLAVVHRQNQPRMLLVAIHHALYDGWSMPLLKQAVEDAYHGRDLRPQPFTPFIEYVIVGKQAAQDFWTTHLDSFAGGVFPNFPSIDYQINPTERRTRSMTLPTVTPRAQYTMATKIQAAWAVTVSRYAEANDIVFGTVSTGRSAPVPAIDRMVGPTITTVPVRISLSNQAERVNSLLQRVQDDSWKKMDHGHLGLQHIRRLGESAAAACSFQTLLVIQPREQPDTKYRSTLLSGLQDVAELKGVDTYPLMLVCEPDGARLHLTAVFDPAVLNGITLERMLAHWELVLIQLWNEPDMAVIELDTVSYSDKEALMRWNTGETIPNGCAHDAVSEWSVRTPHAPAVCAWDGEWTYEELERCSSLIASQILAHGVSSGDFVALYHEKSRWAAAGILAVFKAGAILITLDPAHPTDRIKDILDQARPRLILTSQSLLDVARNFETPLLSVQFAASKPLPEGWSSLPTISSTQAAYTPFTSGSTGRPKGIPLDHRGLAASTASIARSCLLRPASRVLHFASFAFDASMMEHLIAWHAGGCLCIPDDTARQTDLAKCIRGFNVTWAFLTPSCLRLITPDDVPSLQALGLGGESMTSEDITIWGPRLRQIVQLYGPAECCIVAALTPVTKPSENRLIGRPNACRCWVVDSQNLDRMAPIGAVGELVIEGITVGRGYINDPDRTNRAFIRHPKWLQTLYPDDQESKRLYRTGDLVRYAGVDGKLVFIGRRDGQLKLHGQRIELADVEAHLRPLIPGTQKVVVEMVHSADNQNPILAAFLEEMSASQKPTEREVGLLHPSQSQCDQDVKAIDSALSRTVPQYMIPSMYLHISRLPLSASGKLNRRHLREMVAELPRQRLNEYAAGSGLSAPDRPVTSQEREMRAIWARVLSLDPNTIGVNDDFFRIGGDSISGMQVATKCNAAGIHITSADLFRHRNIEQLICHLNTIRTTNCASVSLPTEPVDKWVALAPIQQLFFEVAPEGPNHFNQSLLLHTSRRVSVEELARGLDILVGRHSMLRARFCRKDSGQWFQQVKSLDYEPASGLYRLAAHNQITRESLPTLFTAAQMALSIQDGPLLNVDLVELEDGSQLVYLVAHHLIIDLVSWRILHGELEEYLQTGSLSSATGSVPFLTWSQLQAQYSAEDLTPARALPDFQEANDDFDVTRYWGISSELNTFGQTSISRFTLDRTVTDILFGNANKVMDTRPVEILQAALWYSCNQALTDRPRPSIYVEGHGREPWTDSINVSGTVGWFTTMSPLVSTPWDHLSRKPMRDFVDALSYIKDQRRRIPANGWAYFTSRYLNDEGRIAYGRTKPVVEFLFNYMGQYQEMNRDDAILQLAGDDIQSGTGASDIADNVPRFSLIDVSAFTANGCLTFEFIFPELLQQDARLEQCIKECERTLVAAASSLSEEGPRKTLTDFPLMSALTYDQLSQCLNHALPSIGLRAQDVLNIYPCSPVQQGMLLAQLRDRQAYQQRFRFRIKSQGPTDQLTLKKVKDAWTQVINRHDILRTLLLPVSDHSHFDQVVMVPGSLQHLVRISGTDANPTKDLSHTINITSDSSGTIICEWNVSHALVDAMSIAVIQREVDQELEGALGQHQAPPQYVDYIQWLSLKDNTEAQAYWQRYLKGVEPCLFPKLTSSSEVNPEGTISAIRATWTRDARMDELCHKHAITLTNIFHIVWAVVLGAYVGTDEVCFGYTTLGRDVPVDGVETMVGPLVNVLATTVHLQQDRSILNALLTHQSHLTNSLQHQHHALADVYSSLGLVGSQLFNTIVSLQDMSHFDAPDEQRTRLEMLPANDVSEYNVTLNIGVDQSSIQLVCSYQSASLSAEHANALLRTASHVLREILRDPTQEFCELEVISPECKEQLVKWNAGMLAPTDEYIHEKIQGQCRIHSSREAVCAWDGTFTYAEIDYLSSRLAARLISMGVTSEDIIPIYSPKSRWTVIAILGVLKTGAAFMLLETSHPVARLQAICDQVKTDMIITPASHAVSAANLAPILVVLDNITSSTQEKSDPLPAVGIPPAGEALAYLIFTSGSTGNPKGVMITHENLCSNASIITTSVNMTSDSRVLQFASHAFDGCILELLGALIAGACLIIPSESENKEDLAGCIERMDVTWALLTPSVARILKPETLPRLSNLVLGGEPIAASDLDMWRGHVQVVCAYGPTETTIVASTTSPSTFPADGKDIGLPNGSSLWIVDRWNYHKLSPLGATGELLIEGPNVSQGYLGDPEKTSNAFPDAPRWLSRLRKSPTRLYRTGDLARFDTSTGTIRFVGRKDNQIKFHGQRIELGEIEHHAQLAFSSASTVIVDLITPEQPQQPYIVAFVHQPDANTEATGTIDTILLPPSESFRADALAAQNKMHKRLPHYMVPAVFLPLHRLPLSATGKADRKRLRQCALALSSSELSAYRATASTKRMPSTAAERKMQDLVATVLGRGPAEIGMDDSFFYLGGDSVQAMRLVAEGRQQGLALSLRAIFDSPCLGDLSDQARSLIEDNQRISTASSAGLRDNCDQIDKIVATNSLNKTDVADVLPTTSFQRHWLDGQLKSYIVVDIPGPIDPARLFRAMHRVVEAHPILRVLFVPYETTTLQVILRTAAAIINADLSTTTVEDICRQDADAQVAPGVPYLRVILATQGQADHKIIMRLSHAQYDAVSLSLLMKDLSHAYANDTHPLPSSQFPRFNDYITYQQAQRADPMATTFWRNLLQDVPLTYLNLQPAEPSTSNGTPITLTRDIYIAAFPSLPNGITTATAVKAAWSLVLAQKSDSAAVIFGQVVHGRAIALPGVEGIVGPCANITPVVARLGLQTTGLDLMQSLQDQHRSAMPYEMVDLDDALAYTKKSEAGRKGLQTIVQHQNNVMVDDMEFSLGEVKCGVDVRAVDHVPKEVWVYSSVNEKRPGMLEVKIMSSTLVIGEEVAEELIGLLVEKIMGLLRHPEGVCLNGPC

mlfA

Malformin synthetase mlfA

5101 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

561.81 kDa

MSRFSCIFPTLTDGYVPNPDHTRAAGRRTYRIDLSGWKAPGSETESLILAAWGLVLSSYVGTDEVAFYVVPTTGPDTTALAELKVEGDMPRQSLTYAAHQLLHPGLVGAGQVSGETANTIITFANDIESLFVTQTEESFLSLHVYRDEQGHISLSLTYYLSLLTDAQAANVGTAMAQALAEVGTCDNDKLVKDLSLMSPAHLEHIWRFNADVPGIWEECFHDVIERHAANRPHSLAVDAWDTELTYTDLVREARLLAAYLQRRGVGPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPNLPAGRVDAILEVIEAPFVVTWTKYESFWAERLPTLPIDNYPKPAADATVEALGKPEDLFYVIFTSGSTGRPKGCMLSHTNWLNGALRNAPSWKYGPESRVLQMLSHTFDMSLLEICTSLGSGSCVCVPRPEEIETSISDAINRWQVNHVIMTPSLARALRPDDVPGLKTMCLGGEAFPKEIVTMWSERINLWQFYGPSECSINSSSWPITRPDADPLNIGPPNSAACWVVDVHDYNKLVPVGAIGELLVSGPIVGMGYLKNPVKTAEAFLEEVGFVAKDDPQFGGFRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLEAGVQYAIAMAPQAGLCKNNLIAILTVKGTSTGTQDTAADEIPLLDRRDPIVQETVKKLRSQLQHALPRYMVPTIWAFVGRMPMSASGKIDRVKLRDWVQRMSQETFDAITGRSLEAEEHAFGLSRLEQKIQLAWAEALGLSAAEVGLQQPFVALGGDSIKALDAVARCRARQIKISMVHILSCEGVREAASLAEVQETPAQQVAEMAVDYSNLWTRLSNDYDLDKLGVTQVEEVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGCLPTVERIQQAWASLVERHPSLRTVFVDDLSPEAIYHSIVLRSVPMEVRMREVPRDLSAEAALAIFTEELVPFRANAPLHRMLLLTCRGRVPYLMLEISHVIMDGYALSVFRREFIRACSSSAPLPRGPDYRMFANYHRTRQTDDSARYWTDYLADCVPCHIPTHAVSAPSDGPPEWPRTLQRRDFGFENSAAFLQRCKERQVTLACAIRAAWALVLRAYTQSEDVCFGYVSSGRNVPVPEVETIFGLCLSMQVCRARLSEASTIASLARKIQEDYVASLPFQHYPLAEAQRGLKQTHGQGLFNTAISMEWVPPSAEDEDALLDLEEIREQDDPTEYDIAISVDVHEGHIKLGFLYWPDLTDFEINHLAEALHGAMNCFASHPDEALNTLSLLQASDVCSALSDGPTLLPLEAVRGNVVSMIDRWVTRQPEGAAIDGWDGSMSYKELHEQSSWVARNLLHQGVRLGDRVLVCADRSSRTVATILGIVRAGCVLVLSNPTDPEKRLQWLAKKCNASLVVADPTYEERLATADAHVLSTTSVCAPAAWDYEFPALDEHDLISILFKSGSTGTPKGILMEHGALATSVFLGHGRTLRFSRHTRMLHFASLTFDAALAEIFTTLAHGGCICVPCEEDRLSDVPGCISRFAVNTAMLTPSVGRLLDPGALPTLQTLIMVGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMKPTDEPVNLGYAVAGVRLWVTEAENPNRLAPLGAVGELIVEGRLVTSGYLDDQARTQEAFLPTLPWLPSQHALYRTGDLVRYVDDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQIVVEMVVPAGKMRAQASLVAFVSGLTAADVESSSACNLEGTIPISQIVLPKSAFQALEEVLPRHMIPSVYYALDTIPLSVNGKADRRRLREMGSLLLASSAAHKNNIEGMSKSVKWTPTLELERTLLGLWAATLGLEAETIHGDDSFFELGGDSVSAMKLVATARDKYKLSLSVPQMFRYPTVCQLAAEVGEPAGQSASSASSTTEEGFTFSTPDDSSTNDGVDDDFLQLATAQLAQLAQEKGKKVDIAALLKQLQGGSSSNKTPSVSSSSSSSSSSKRKKNAAKAESLAEAAAPIPVQFSLLDGGADALDKVRAQAVEHCKITHDDIEDIYPATALQEGMMALTARTPGVYTTTLTGDLSEQVDIARLQYAWGKAAEAHPILRTRIILTDNNTAVQVVQRAKGLPWDTYSLREDNVLPDLTSNMTSGSPLLRLAVVHRQSQPRMLLVAIHHALYDGWSMPLLKQAVEDAYHGRDLRPQPFTPFIKHLIAGKPAAQDFWTTHLDNFVGGVFPKLPSIYHQIQPTERRTRSMTLPTAAPKAQYTMATKIQAAWAVTVSRYVEANDIVFGTVSTGRSAPVPAIDRMVGPTVTTVPVRISLGGQADRVLSLLQRVQEDSWNKLDHEHLGLQHIRRLGESAAAACNFQTLLVIQPREQPDTKYRSTLLSGLQDVAELEGVDTYPLMLVCEPDGASLNLTAVFDRAVLDGATLDRMLAHWELVLTQMWNEPNMAVIDIDAVSCSDKETLMRWNTGETITEGCAHNAVCEWSRRTPHAPAVCAWDGEWTYKELERYSSLIASQISAHGLSSGDFVALYHEKSRWTAAGILAVFKAGAILITLDPAHPTDRIKDILDQARPRLILTSQSLLDVARNLDTPVLSVQFAASQPLPEEWSSLPTICPTLAAYAPFTSGSTGRPKGIPLDHRGLAASTASIARSCLLRPASRVLHFASFAFDASMMEHLIAWHAGGCLCIPDETARQTDLAKCIRDFNVTWAFLTPSCLRLITPDDVPSLQALGLGGESMTSEDITIWSPRLRQIVQLYGPAECCIVAALTEVTKPSENRLIGRPNACRCWVVDPQNPDRLAPIGAVGELLIEGITVGRGYINDPDRTTPAFIRPPKWLQTLYPDDQEPKRLYRTGDLVRYAGVDGKLAFIGRRDGQLKLHGQRIELADVEAHLRSLIPGMQKMVVEMVHSADNQNPFLAAFLEEISTSQKPKEREIGLLHPSQSQCALDVKAIDSALSRTVPQYMIPSMYLHISRLPLSASGKLDRRHLREMVAELPRQSLNEYAAGSGLGVPDRPVTSQEHEMQAIWARVLSLDPNTFGVNDDFFRIGGDSISGMQVSTKCNAAGIHITSADLFRHRTIEQLICHLNTIRTTDSASVLLPTEPVNEWVALAPIQHLFFEVAPEGPNHFNQSLLLRTSRRVSVEELAGGLDVLIGRHSMLRARFCRKDSGQWFQQVKSLDSEPASAFYRLAAHNHITRESLPTLFTAAQMALSIEDGPLLTVDLVELEDGSQLVYLAAHHLIIDLVSWRILHGDLEEYLQTGSLSSATGSVPFLTWTQLQAEYSAEHLTPARALPGFQEANDDFDFMRYWGISSESNTFGQTSTSRFALDRTVTDILFGSANKVMDTRPVEILEAALWYSCNQALPDHPGPSIYVEGHGREPWTDSIDVSGTVGWFTIMSPLVSTPWHHLSRKSMRDFVDVLSYIKDQRRRIPANGWAYFTSRYLNDEGRVAYGRTKPVMEVLFNYMGQYQEMKREDAILQLAGDDIQSGTGASDIAGNVPRFSLIDVTAFTANGCLTFEFTFPQLIQQDARLEQCIKECEHTLVAAASSLSAEGPRKTLTDFPLMSALTYDQLSQCLNHTLPSMGLRAQDVWNIYPCSPVQRGMLLAQLRDRQAYQQRFKFQVMSRGPTEQLSLEKVKDAWTEVINRHDILRTLLLPVSDHSHFDQVVMVPGSLQHLVRGDAMDANPTEGLPHTINITSDSTGAIICEWNVSHALVDAMSIAFIQREVNQALEGSLGQHQNLPQYVEYIKWLTLQDNTEAQAYWQNHLNGVEPCLFPKLTSSPDKVNPEATISAIRATWSRDVRMDELCHKHAITLTNLFHIVWAIVLGAYVGTDEVCFGYTALGRDVPVHRVETLVGPLVNVLATTVRHQEDETILNALLTHQAHLTNSLQHQHYALADVYASLGLVGSQLFNTIVSLQDTSHFDAPDEQRTRLEMLPANDVSEYDVALNIGVDKSTIQLVCSYQTVSLSAEQADALLRTAFHVLDEILRDPTQRFCELEVISPKCKEHLVKWNAGMLAPTHEYIHEKIQGQCRIHNSRQAVCAWDGMFTYAEVDDLSSRLAARLIRMGVTSEDIIPIYSPKSRWMVIAILGVLKAGAAFTLLEISHPMARLRVICNQIKAPMLIAPASHAVPAANLAPILVVLDNITSLAEERPVSLPAVDIPPAREALAYLIFTSGSTGNPKGVMVTHQNLCSNASIITTSVNMTSDSRVLQFASHAFDGCLWEILGALLAGACLIIPSESENKEDLTGCIERMGVTWAFLTPSVARILKPETLPSLCNLVLGGEPIAASDLEMWRGHVQVVCAYGPTETTILASTTSPSTFPRDGKDIGTPTSSSLWIVDTRNYQTLVPLGATGELLIEGPNVSQGYLGDPEKTNNAFPDAPRWLSQLRKSPTRLYRTGDLVRFDTSTGTIRFVGRKDNQIKFHGQRIELGEIEYHAQFAFSSASTVIVDLITPEQPRQPYIVAFVHQLDAANETTDTNDTLLLPSSEVFRADALAAQNKMHKRLPHYMVPAVFLPLHRLPLSVTGKADRKRLRQCALALSSPELSAYRATASTKRMPSTAAERKMQELVATVLGRDPTEIGMDDSFFYLGGDSVQAMRLVAEGRQQGLTLSLRAIFDSPCLGDLSDQAKSLIEDNQRASTASRGNLRYDCDRIDKIVVTNSLNKADVVDVLPTTSFQRHWLDAQLKSYIVVDIPGPIDPARLLRAMHRVVEAHPILRVSFVPYETTTVQVILRTAVAITNVDLSTATVEELCRRDVDAQMAPGVPYLRVIIATQDKAGHKLIMRLSHAQYDAVSLSLLMNDLSHAYANDTHPLPSSHFPRFNDYITYQQAQRADPTATTFWRHLLQDVPLTHLNLQPAESSASNGTPITLSRDIDIAVFPSLPSDITIATMVKAAWSLALAQKTNSLAVIFGQVVHGRAIALPGVEGIVGPCANITPVVARLGLETTGLELMQALQDQHHSAMSYESVDLDDALAYANDSQAGRKGLQTIVQHQNNVMVDDMELSLGEVKCGVDFRAVDHLPKEVWVYSSVDEKRPGMLEVKIMSSTLVLGEEFAEELMGLLVEKIVGLLRHPESVCV

mlfA

Malformin synthetase mlfA

5099 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

560.492 kDa

MGRFSCIFPSLTDGYIPDPAHCRAAGRRVYEIDLGGWGPLPDRPDAHLVAAWALILSSYVGTDEVAFYVVPARGPDATALCELKVNGSLPRRSLTDDAWQLLHPLPLGPGQVSSETANTIITFAEDIDPLFITQAEEAFLTLHVRNTSPGNVALHLGYHLSLFTDAQAANVGTAMAQVLTSLAGDPDELVRDVDHMSRTHLDQIWHFNANVPSTWQECFHDVVQRHAADRPHSLAIDAWDGRLTYAELVGKATPLARHMQERGVRPGVVVPISFERSAGALIAMLAVSKAGGAFVSVPTSLPPGRLDAILEVIEAPFVLTRSTHQSFWAGRLPALIIDNYPKPASTAVVETLAKADDLFYVIFTSGSTGRPKGCMLSHSNWLNGALRNAPNWKYGPNSRVLQMLNHTFDMSLLEICTSLGSGACVCIPPADEVEAGLAGAINRWQVNHVIMTPSLARALRPGDVPGLRTMCLGGEAFPREIVTMWSECIHLFQFYGPSECSINSSTRAITGVDADPLNIGPPNSAACWVSDIQDHNKLVPIGAIGELLVSGPIVGMGYLRNPVKTAEAFIDHVGFIPMDDPKFAGFRLYKTGDLVRWNSDGTLTFCGRADTQVKLNGQRLELAEVEYQLGLEADVQLAIALVPQVGRCKNNLIAILTVRGAATSSRGVTAGEIPLLNRQDPIVQQAVKRLRAQLQQALPRYMVPTIWAFVGHMPMSASGKIDRVRVRGWVEEMSQETFDAITGRSFEADDHLLGLTHLENEIQLAWAEALGLSAAEVGLHQPFVALGGDSIKALDAVGRCRARQVDITMVSTLSCEGVREAASLAKVRDSPTQRVVEMAVDYSDLWDCLSSDYDLAKLGIGNADEVEDVFPCTSMQEGMFLGQIRRPGAYHMRFFHRVQLKGGGLPTVERIQTAWSSLVARHPSLRTIFVDDLSPEAIYHSVVLRNVPVDTTTREVPRDLSPEDALSMFTQELVPFRPNAPLHRLRLFTCRGRVSYFMLEISHVIMDGYALSVFRREFIQACSTPASVPRGPDYRMFANYHRTRQTADSAAYWTAYLKDAAPCHIPTYDQAMAADGLDYPRTLLRRDFSYQTSGTFLQRCKERQVTLACAIRATWALVLRAYTQSQDVCFGYVSSGRNVPVPDVETIFGLCLSMQVCRARVGESRTLVDLARRIQEDYVESLPYQHYPLAEVQRGLKQTRRQALFNTAISMEWVPPAGDDEDALIDLEEIREQDDPTEYDIAISVDVHAGCIKLGFLYWPTLTEFEITHLAQAMQGAMDCFALQPDGPVDSLTLLKPGDLSSALVGWPDLLPLEAVRGNVMSMIDRWVNRQPDTLAIDGWDESLTYHQLQQQSSWVARNLLHRGVHQGDRILVCMDRSSRTVVTILGIVRSGAVLVLSNPADPAKRLQWLTQKCNAAMIVADPQYRDRFEAPGNPSVTVVDAPSVCTPAAWDYLFPVLDGQDPVSILFTSGSTGTPKGIVMHHGSLATSVLLGHGRTLRFSRQTRMLHFASLTFDAALAEIFTTLAHGGCMCIPSEDDRLSDVPGCIARFKVNTAMLTPSVGRILDPAALPTLRTLVLVGEPMSRLDVERFAPALDLYNGAGPTETSIMVTIAGPMQPTDDPLNLGHAVAGVRLWVTETEAPNRLAPLGAVGELVVEGSLVTQGYLDDPVRTQEAFLSKLAWLPSHNPLYRTGDLVRYVADGSFRYMGRKDTQVKLRGQRIELQEVEYHLRCSWPHAQVVVEMVIPDGRTRSQAALVAFVSGLTPEDAHFLFNGALISAEAPGIAQAVLSEKTTQALSEALPRHMVPSIYLALETIPLSVNGKADRRRLRDLGASWLASSAFHPGPECLQTPTAEWARAPELERTLVELWATTLSIEPGAIRGDDSFFELGGDSVSAMKLVATARDKYKLSLSVPQVFRYPTIRQIATQCEGIAVQLASSASSTTEEGFTFSTPDESSTNEGLDGEFWQLASAQLADLAREKGKTLDVAAILKRMQQESSSSPAPSVSSSSSSSSAPKPLLAQPEPPTNLRDSVPEPFSLIGGGPSAVEQICQQAMEQCQIPRESIEDIYPATPLQEGMMALMAKTPGVYTTTLRCELSNQVDCARLQSAWDQASEAHPILRTRIVLTNDHRAMQVVQHGNRLPWDVYSLRDSDNLPDLTSQMTLGSPLLRLAEIRQAGGQGRLLLVTIHHALYDGWSFPLVKQAVEDAYSGQALKSQAFTPFIAYLNEGRLAAEQFWADHLESFAGGAFPCLPTVDHRIRPTARLTRKLSLPVSAGHQYTLATKIQAAWAVTVSRYDDETDVVFGTVSTGRAAPVPGIDRVAGPTITTIPVRVSLQDRAQRVGPFLQKVQEDGWRSLDHEHLGLQHIRRLGDSAAAACRLQTLLVLQPRQQPPAEPSSAILAGLQDMAELEGLDTYPLMLVCEPDGADVHLIAIFDPVVLHEAILARMVTHWEHVLTQLWTEPDIAVVDLEALSPGDKKVLMRWNGASQLPDGCVHESVHQWRLSTPHAPAVCAWDGDWTYEELDNLSSALAHHLTLHGVSHGTSVALYHEKSRWAAVGLLAVFKAGGILVTLDPAHPVDRLREILGQVQARVILSSQEHEATAKALGTLVLTVEEVATQPEPELFRPVGPITSSQCAFTPFTSGSTGRPKGIPLEHRGMVATIASMAERCLLTTTSRVLQFASFAFDASVMEHLLAWYAGGCLCVPSEFDRQANLGEVIRDLRVNWAFLTPSCLRLITPDDVPCLEGMGLGGEPVLPEHITTWAPRLRQLVQMYGPAECSFVTVLTEVTQASENRLIGSPSACRCWVIDPMDPDRLMPLGAIGELVIEGLAVGRGYINEPQRTAEAFIAPPPWLQTLYPDDGQPRRLYRTGDLVRYAGDDGRLTFVGRKDGQLKLHGQRIELGDVETHLRPLIPATQGIAVEMIVCADDQNPLLAAFIEVSQDATALQRNTHLVHPGRVQSAVDIKAIESTLARTVPHYMVPSIYLHISKLPLNPSGKLNRRQLREMVGALPRQSLNEYAIKCHSSTTNRPATAQERGLQTIWATVLALDRDAIGVHDDFFRMGGDSIAGMQVATKCNAAGMRISSADLFRHRTIARLVLHLQNTSQESSAMITLPEEKFDEWVHLAPIQQLFFENAPDGPNHFNQSLLLRTGRQVDAQELAAGLDILVQRHSMLRARFRRTDSGRWTQQVMSLGPSPSSFYRLFTHGKASPETLPGIFTASQSAIDIQKGPLLSVDLVDLTDGSQLVYFVAHHLVIDLVSWRILHAELEDYLRTGSLAAMAESTPFLTWCRAQAEYSAKELSPAQSLPGYQSAANHFDPEGYWGISMESNTFTQVASYRFTLDGDTTETLFGVANDALGTQPVEIMLAALWYSFTQTLTGRPEPSIYVEGHGREPWTDTIDLSGTVGWFTTMSPLVSSPWSNLSQASMRDFADALRYIKDQRRRVPANGWAYFASRYLNDEGRVVYGRTNPAVEILFNYMGQYQQLTREDAILQLVGDDIQAGTGAADIAGDVRRFALIDVSAFTAHGCLSFDFSFPESIQHRDRLQHWFEQCRQTLIVAASILSLQTPQKTLTDFPLLPSLTYDQLSQCLDHTLPAVGLFPSDIVDIYPCSPVQRGMLLAQLRNPQFYQQRFRFRVLPDTRTEAIGLVGLQQVRDAWIEVINRHDILRTIFLPVSDQGYVDQVLLKPGSLHHLVRIGAGEPDTAIDPGTPHWVNISHDPTGTVVCDWNVSHALVDAVSVAIIQREVSQALQGHLSTPPPQQYGNYIQWLSAQNMQETQDYWKKYLQAVEPCLFPRLAAHSDRLTSPVGIQATRATVDREVRIDQLCHQHGITLTNVFHLVWALVLRTYVGTNNVCFGYATQGRDVPVAGVEEMVGPVVNVLATTLRLQDSESVLDALQTHQTHLADNLAHQNHALVDIYATHGVAGSQLFNTIVSLQDLSHHETADTQSLRLEVMPAHDSSEYDIALNVGVNKTSIELVCSYQTWSLSAEHADALLRTAARVLDEILQTPLKTIGEVDVVSLTCKQQAMRWNATLPATVHEYVHEQIQAQCRLYANREAVCAWDGHFTFAEIDDLSSRLAGNLISLGAQPGRIVPIYSPKSRWVVIAILGVLKAGAAFTLLDASHPVARLQEICQGVNADLIISLASHASVAAQLAAAIVILDNAPSMSSDEIIMCTRRQSLSTESLAYVIFTSGSTGKPKGVMVSHSNLCTSAMAQTVALNLTTESRVLQFASYAFDACILEVIGALFAGACVCIPSETDSKDDLVGSIERMRVTWALLTPSVARILNQETLPTLETLVLGGEPITLSDLEPWRSRLQLVCAYGPSECTIVSTTTALSTFPNRSKNIGQRGACATWVVDPQDYQKLVPFGATGELLIEGPIVGQGYLGDAVRTAECFPPPPRWLSQLRQAPTRVYRTGDLVRYESDGTIRFVGRKDSQIKFHGQRIELGDIEHHAQDAFRDAQTVIIDLITPGELHKAYLAAFVLQKDTDAHLDKVDDDCILLSPSDRFRSRSLAAQEQMHEELPHYMVPAIFLPLSRVPLAKTGKTDRQYLRQCALALQGHDLEAYRAASSTKRPPSTGMQQGIQALVATVLGKDTTEVGMDDSFFHLGGDSVQAMRLVSVGRQQGLAVSVLSIFDHPRLADLAEHISSRVKDRNSVRLPSSSLLPSISEAGQLDRLVEGHPFEKDDVVDILPTTGFQRYWLDMQLSSYVIIDIPGQVDWAQLTKALQRVIENCPILRTAFVPYRGSTVQVILKTIVSLIEVDLTGDLTSAVEEWCRQDAKTPVSPGTSYMHTVRASQGATQHKLIMRLSHAQYDAVALSLVLNHLSCTYADQLPLPDAPAFSDYLTYQRTRNKERASSDFWGQLLKGASITNLGPQGSRDDKEATIARSRHISVGPLPRGITMATVVKAAWSLILARRTGMCDVLFGQVVHGRNTSFPGIERVVGPCTNITPVRASIAPQWTGLDLLQALQDQHRESMAWETVDLDDVLSYCANWTPGSALQTVVQHQNVVLEDLGLRLGDVPSTVSVRAFDHAPREVWLYSSTDDSHPDRLSLRIFSSSWTLDDTIAEELLGLVAEEIVELLRNPEQVLCV

mlfA

Malformin synthetase mlfA

5082 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

559.619 kDa

MSRFSCIFPTLTDGYVPNPDHTRAAGRRTYRIDLSGWKAPGSETESLILAAWGLVLSSYVGTDEVAFYVVPTTGPDTTALAELKVEGDMPRQSLTYAAHQLLHPGLVGAGQVSGETANTIITFANDIESLFVTQTEESFLSLHVYRDEQGHISLSLTYYLSLLTDAQAANVGTAMAQALAEVGTCDNDKLVKDLSLMSPAHLEHIWRFNADVPGIWEECFHDVIERHAANRPHSLAVDAWDTKLTYTDLVREARLLAAYLQRRGVGPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPNLPAGRVDAILEVIEAPFVVTWTKYESFWAERLPTLPIDNYPKPAADATVEALGKPEDLFYVIFTSGSTGRPKGCMLSHTNWLNGALRNAPSWKYGPESRVLQMLSHTFDMSLLEICTSLGSGSCVCVPRPEEIETSISDAINRWQVNHVIMTPSLARALRPDDVPGLKTMCLGGEAFPKEIVTMWSERINLWQFYGPSECSINSSSWPITRPDADPLNIGPPNSAACWVVDVHDYNKLVPVGAIGELLVSGPIVGMGYLKNPVKTAEAFLEEVGFVAKDDPQFGGFRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLEAGVQYAIAMAPQAGLCKNNLIAILTVKGTSTGTQDTAADEIPLLDRRDPIVQETVKKLRSQLQHALPRYMVPTIWAFVGRMPMSASGKIDRRMSQETFDAITGRSLEAEEHAFGLSRLEQKIQLAWAEALGLSAAEVGLQQPFVALGGDSIKALDAVARCRARQIKISMVHILSCEGVREAASLAEVQETPAQQVAEMAVDYSNLWTRLSNDYDLDKLGVTQVEEVENVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGCLPTVERIQQAWASLVERHPSLRTVFVDDLSPEAIYHSIVLRSVPMEVRMREVPRDLSAEAALAIFTEELVPFRANAPLHRMLLLTCRGRVPYLMLEISHVIMDGYALSVFRREFIRACSSSAPLPRGPDYRMFANYHRTRQTDDSARYWTDYLADCVPCHIPTHAVSAPSDGPPEWPRTLQRRDFGFENSAAFLQRCKERQVTLACAIRAAWALVLRAYTQSEDVCFGYVSSGRNVPVPEVETIFGLCLSMQVCRARLSEASTIASLARKIQEDYVASLPFQHYPLAEAQRGLKQTHGQGLFNTAISMEWVPPSAEDEDALLDLEEIREQDDPTEYDIAISVDVHEGHIKLGFLYWPDLTDFEINHLAEALHGAMNCFASHPDEALNTLSLLQASDVCSALSDGPTLLPLEAVRGNVVSMIDRWVTRQPEGAAIDGWDGSMSYKELHEQSSWVARNLLHQGVRLGDRVLVCADRSSRTVATILGIVRAGCVLVLSNPTDPEKRLQWLAKKCNASLVVADPTYEERLATADAHVLSTTSVCAPAAWDYEFPALDEHDLISILFTSGSTGTPKGILMEHGALATSVFLGHGRTLRFSRHTRMLHFASLTFDAALAEIFTTLAHGGCICVPCEEDRLSDVPGCISRFAVNTAMLTPSVGRLLDPGALPTLQTLIMVGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMKPTDEPVNLGYAVAGVRLWVTEAENPNRLAPLGAVGELIVEGRLVTSGYLDDQARTQEAFLPTLPWLPSQHALYRTGDLVRYVDDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQIVVEMVVPAGKMRAQASLVAFVSGLTAADVESSSACNLEGTIPISQIVLPKSAFQALEEVLPRHMIPSVYYALDTIPLSVNGKADRRRLREMGSLLLASSAAHKNNIEGMSKSVKWTPTLELERTLLGLWAATLGLEAETIHGDDSFFELGGDSVSAMKLVATARDKYKLSLSVPQMFRYPTVCQLAAEVGEPAGQSASSASSTTEEGFTFSTPDDSSTNDGVDDDFLQLATAQLAQLAQEKGKKVDIAALLKQLQGGSSSNKTPSVSSSSSSSSSSKRKKNAAKAESLAEAAAPIPVQFSLLDGGADALDKVRAQAVEHCKITHEDIEDIYPATALQEGMMALTARTPGVYTTTLTGDLSEQVDIARLQYAWGKAAEAHPILRTRIILTDNNTAVQVVQRAKGLPWDTYSLREDNVLPDLTSNMTSGSPLLRLAVVHRQSQPRMLLVAIHHALYDGWSMPLLKQAVEDAYHGRDLRPQPFTPFIKHLIAGKPAAQDFWTTHLDNFVGGVFPKLPSIYHQIQPTERRTRSMTLPTAAPKAQYTMATKIQAAWAVTVSRYVEANDIVFGTVSTGRSAPVPAIDRMVGPTVTTVPVRISLGGQADRVLSLLQRVQEDSWNKLDHEHLGLQHIRRLGESAAAACNFQTLLVIQPREQPDTKYRSTLLSGLQDVAELEGVDTYPLMLVCEPDGASLNLTAVFDRAVLDGATLDRMLAHWELVLTQMWNEPNMAVIDIDAVSCSDKETLMRWNTGETITEGCAHNAVCEWSRRTPHAPAVCAWDGEWTYKELERYSSLIASQISAHGLSSGDFVALYHEKSRWTAAGILAVFKAGAILITLDPAHPTDRIKDILDQARPRLILTSQSLLDVARNLDTPVLSVQFAASQPLPEEWSSLPTICPTLAAYAPFTSGSTGRPKGIPLDHRGLAASTASIARSCLLRPASRVLHFASFAFDASMMEHLIAWHAGGCLCIPDETARQTDLAKCIRDFNVTWAFLTPSCLRLITPDDVPSLQALGLGGESMTSEDITIWSPRLRQIVQLYGPAECCIVAALTEVTKPSENRLIGRPNACRCWVVDPQNPDRLAPIGAVGELLIEGITVGRGYINDPDRTTPAFIRPPKWLQTLYPDDQEPKRLYRTGDLVRYAGVDGKLAFIGRRDGQLKLHGQRIELADVEAHLRSLIPGMQKMVVEMVHSADNQNPFLAAFLEEISTSQKPKEREIGLLHPSQSQCALDVKAIDSALSRTVPQYMIPSMYLHISRLPLSASGKLDRRHLREMVAELPRQSLNEYAAGSGLGVPDRPVTSQEHEMQAIWARVLSLDPNTFGVNDDFFRIGGDSISGMQVSTKCNAAGIHITSADLFRHRTIEQLICHLNTIRTTDSASVLLPTEPVNEWVALAPIQHLFFEVAPEGPNHFNQSLLLRTSRRVSVEELAGGLDVLIGRHSMLRARFCRKDSGQWFQQVKSLDSEPASAFYRLAAHNHITRESLRTLFTAAQMALSIEDGPLLTVDLVELEDGSQLVYLAAHHLIIDLVSWRILHGDLEEYLQTGSLSSATGSVPFLTWTQLQAEYSAEHLTPARALPGFQEANDDFDFMRYWGISSESNTFGQTSTSRFALDRTVTDILFGSANKVMDTRPVEILEAALWYSCNQALPDHPGPSIYVEGHGREPWTDSIDVSGTVGWFTIMSPLVSTPWHHLSRKSMRDFVDVLSYIKDQRRRIPANGWAYFTSRYLNDEGRVAYGRTKPVMEVLFNYMGQYQEMKREDAILQLAGDDIQSGTGASDIAGNVPRFSLIDVTAFTANGCLTFEFTFPQLIQQDARLEQCIKECEHTLVAAASSLSAEGPRKTLTDFPLMSALTYDQLSQCLNHTLPSMGLRAQDVWNIYPCSPVQRGMLLAQLRDRQAYQQRFKFQVMSRGPTEQLSLEKVKDAWTEVINRHDILRTLLLPVSDHSHFDQVVMVPGSLQHLVRGDAMDANPTEGLPHTINITSDSTGAIICEWNVSHALVDAMSIAFIQREVNQALEGSLGQHQNLPQYVEYIKWLTLQDNTEAQAYWQNHLNGVEPCLFPKLTSSPDKVNPEATISAIRATWSRDVRMDELCHKHAITLTNLFHIVWAIVLGAYVGTDEVCFGYTALGRDVPVHRVETLVGPLVNVLATTVRHQEDETILNALLTHQAHLTNSLQHQHYALADVYASLGLVGSQLFNTIVSLQDTSHFDAPDEQRTRLEMLPANDVSEYDVALNIGVDKSTIQLVCSYQTVSLSAEQADALLRTAFHVLDEILRDPTQRFCELEVISPKCKEHLVKWNAGMLAPTHEYIHEKIQGQCRIHNSRQAVFDDLSSRLAARLIRMGVTSEDIIPIYSPKSRWMVIAILGVLKAGAAFTLLEISHPMARLRVICNQIKAPMLIAPASHAVPAANLAPILVVLDNITSLAEERPVSLPAVDIPPAREALAYLIFTSGSTGNPKGVMVTHQNLCSNASIITTSVNMTSDSRVLQFASHAFDGCLWEILGALLAGACLIIPSESENKEDLTGCIERMGVTWAFLTPSVARILKPETLPSLCNLVLGGEPIAASDLEMWRGHVQVVCAYGPTETTILASTTSPSTFPRDGKDIGTPTSSSLWIVDTRNYQTLVPLGATGELLIEGPNVSQGYLGDPEKTNDAFPDAPRWLSQLRKSPTRLYRTGDLVRFDTSTGTIRFVGRKDNQIKFHGQRIELGEIEYHAQFAFSSASTVIVDLITPEQPRQPYIVAFVHQLDAANETTDTNDTLLLPSSEVFRADALAAQNKMHKRLPHYMVPAVFLPLHRLPLSVTGKADRKRLRQCALALSSPELSAYRATASTKRMPSTAAERKMQELVATVLGRDPTEIGMDDSFFYLGGDSVQAMRLVAEGRQQGLTLSLRAIFDSPCLGDLSDQAKSLIEDNQRASTASRGNLRYDCDRIDKIVVTNSLNKADVVDVLPTTSFQRHWLDAQLKSYIVVDIPGPIDPARLLRAMHRVVEAHPILRVSFVPYETTTVQVILRTAVAITNVDLSTATVEELCRRDVDAQMAPGVPYLRVIIATQDKAGHKLIMRLSHAQYDAVSLSLLMNDLSHAYANDTHPLPSSHFPRFNDYITYQQAQRADLTATTFWRHLLQDVPLTHLNLQPAESSASNGTPITLSRDIDIAVFPSLPSDITIATMVKAAWSLALAQKTNSLAVIFGQVVHGRAIALPGVEGIVGPCANITPVVARLGLETTGLELMQALQDQHHSAMSYESVDLDDALAYANDSQAGRKGLQTIVQHQNNVMVDDMELSLGEVKCGVDFRAVDHLPKEVWVYSSVDEKRPGMLEVKIMSSTLVLGEEFAEELMGLLVEKIVGLLRHPESVCV

mlfA

Malformin synthetase mlfA

5073 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

558.19 kDa

MSRFSCIFPTLTDGYIPKPDHTCAAGRRTYTIDLSGWKAPGSETESHILAAWGLVLSSYVGTDEVAFYVVPTTGPDTTALADLKVEGDMSRQSLTYAAMQLLHPALVGAGQVSGETANTIITFAKDIESLFVTQTEAANVGTAMAQALAEVGTCDNDRIIKDLNLMSPAHLEHIWRFNANVPGIWEECFHDVIERHATNRPHSLAVDAWDTKLTYADLVREARLLAAYLQQRGVGPGSVVPISFERSGAALVAMLAVSKAGSAFVSVPPNLPAGRLDAILDVIEAPFVVTWTKYESFWAERLPTLPIDNYPKPAADATVEALGKPEDLFYVIFTSGSTGRPKGCMLSHSNWLNGALRNAPSWKYGPESRVLQMLSHTFDMSLLEICTSLGSGACVCVPRTEEIETSISDAINRWQVNHVIMTPSLARALRPDDVPGLKTMCLGGEAFPKEIVTMWSERINLWQFYGPSECSINSSSRPITRPDADPLNIGPPNSAACWVTDVHDYNKLVPVGAIGELLVSGPIVGMGYLKNPVKTAEAFLEEVGFVAKDDPQFGGFRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLESGVQYAIAMAPQAGLCKNNLIAILTVKGTSTGNQDTAADEIPLLDRRDPIVQETVKRLRSQLQHALPRYMVPTIWAFVGRMPMSASGKIDRVQLRDWVQKMSQETFDAITGRSLEAEDHVLGLSRLEQEVQLAWAEALGLSAAEVGLQQPFVALGGDSIMALDAVARCRARQIKISMVHILSCEGVREAASLAEVQETPAQQVAEMAVDYSNLWTRLSDDYDLDKLGVTQLEEVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGCLPTVERIQQAWASLVERHPSLRTVFVDDLSPEAIYHSIVLRSVPMELRMREVPRDLRAEAALAMFTEELVPFRANAPLHRMLLLTCRGRVPYFMLEISHVIMDGYALSVFRREFIRACSSSAPLPRGPDYRMFANYHRTRQTDDSARYWTNYLADCVPCHIPTHAVSAPSDGPPEWPRTLQRRDFGFDNSAAFLQRCKERQVTLACAIRAAWALVLRAYTQSQDVCFGYVSSGRNVPVPEVETIFGLCLSMQVCRARLSEASTIASIAKKIQEDYVASLPFQHYPLAEAQRGLKQTHGQGLFNTAISMEWVPPSAEDEDALLDLEEIREQDDPTEYDIAISVDVHEGHIKLGFLYWPNLTDFEITHLAEALQGAMNCFVFQPDEALNTLSLLQASDVCSALADGPTLLPLEAVRGNVVSMIDRWVTRHPEGAAIDGWDGSLTYKELHEQSSWVARNLLHQGVQLGDRILVCADRSSRTVATVLGIVRAGCVLVLSNPTDPAKRLQWLAKRCNAALIVADPTYEERFATADARVLSTTSVCAPAAWDYEFPALDEHDLISILFTSGSTGTPKGILMEHGALATSVLLGHGRTLRFSRHTRMLHFASLTFDAALAEIFTTLAHGGCICVPCEEDRLSDVPGCISRFAVNMAMLTPSVGRLLEPGALPTLKSLIMVGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMKPTDEPVDLGYAVAGVRLWVTEAENPNRLAPLGAVGELVVEGRLVTRGYLDDPARTHEAFLPSLPWLPSQHALYRTGDLVRYADDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQIVVEMVVPAGKMRAQASLVAFVSGLTAADVESSSACNLEGMIPISQIVLPKSAFKALEEALPRHMIPSVYYALDTIPLSVNGKADRRRLREMGSLLLASSAAHKNTIEGMSKSVKWTPASELERTLLELMAATLGLEAETMHGDDSFFELGGDSVSAMKLVATARDKYKLSLSVPQMFRYPTICQLAAEVGEPAGQSASSASSTTEEGFTFSTPDDSSTNDGVDDDFLQLATAQLAQLAQEKGKKVDIAALLKQLQGGSSSNKTPSVSSSSSSSSSSKRKKKAALAEAAAPISVQFSLLDGGADVLDKVRAQAVEHCKIPHEDIEDIYPATALQEGMIALTARTPGVYTTTLTCDLSEQVDLARLHYAWGKAAEAHPILRTRIILTDNNTAVQVVQRAKGLPWDTYSLREGDVLPDLTSNMTSGSPLLRLAVVHRQNQPRMLLVAIHHALYDGWSMPLLKEAVEDAYHGRDLRPQPFTPFIKHLIAGKVAAQAFWTTHLDSFAGGVFPNLPGVDHQVQPKERRTRSLTMPTATPRAQYTMATKIQAAWAVTVSRYAEDNDVVFGSVSTGRSAPVPAIDRMVGPTITTVPVRISLGDQAERLTSLLQRVQDDSWERMDHEHLGLQHIRRLGESAAAACGFQTLLVIQPREQPNNKYRSTLLSSLQDVAELEGVDTYPLMLVCEPDGARLHLSAVFDPVVLDGVTLERMLANWELVLTQLWNEPDMAVLELDAVSCSDTETLIRWNTGETIADGCAHDAVCEWSSRTPHAPAVCAWDGEWTYEELERCSSLVASQILAHDVSSGDFIALYHEKSRWAAAGILAVFKAGAILITLDPAHPTDRIKNILDQARPRLILTSQSLLDVARNLETPALSVQFAASQPLPEGWSSLPTISSTQAAYAPFTSGSTGRPKGIPLDHRGLAASTASIAHSCLLRPASRVLHFASFAFDASMMEHLIAWRAGGCLCIPDETARQTDLAKCIRDFNVTWAFLTPSCLRLITPDDVPSLQALGLGGESMTSEDIAIWSPRLRQIVQLYGPAECCIVAALTEVTKPSENRLIGRPNACRCWVVDLQNPDRLAPIGAVGELLIEGITVGRGYINDPDRTTPVFIRPPKWLQTLYPDDQEPKRLYRTGDLVRYADVDGKLAFIGRRDGQLKLHGQRIELADIEAHLRPLIPATQKMAVEMLHSADNQNLILAAFLEEMSTSQKPTEREVKLLHPSQSQCALNVMAIDSALSRKVPQYMIPSMYLHISRLPLSASGKLDRRHLREMIAELPRQRLNEYAAGSGLRVPDRPKTSQEQEMQAIWARVLSLDPNTIGVNDDFFRIGGDSISGMQVATKCNAAGIHITSADLFRHRTIEQLICHLNSIRTTDCASVSLPAEPVDEWVALAPIQQLFFEVAPEGPNHFNQSLLLRTSRRVSVEELAGGLDILVGRHSMLRARFCRKDSGQWFQQVKSLGSEPASNFYRLAAHNQITRESLPTLFTTAQMALSIQDGPLLTVDLVELEDGRQLVYLAAHHLIIDLVSWRILHGELEEYLQTDSLSSATGSVPFITWSQLQAEYSAEHLTPARAFPGFQEANDDFDVMRYWGISSESNTFGQTSISRFTLDRTVTDILFGSANKVMDTRPVEILQAALWYSCNQALTDRPGPSIYVEGHGREPWTDAINVSGTVGWFTTMSPLVSTPWDHLSRTSMRDFVDALSYIKDQRRRIPANGWAYFTSRYLNDEGRVAYGRTKPVVEVLFNYMGQYQEMNREGAMLQLAGNDIQSGTGASDIADNVPRFSLIDVSAFTANGCLTFEFTFPQLMQQDARLEQCIKECERTLVAAASSLSAEGPRKTLTDFPLMSALTYDQLSQFLDHTLPSLGLRAQDVLDIYPCSPVQQGMLLAQLRDRQAYQQRFRFQVMSRVPTDQLPLEKVKGAWTEVINRHDILRTLLLPVSDHSHFDQVVMVPGSLQHLVRLNAMDTNPADGLPHTINITSDSADTIICEWKVSHALVDAMSIAVIQREVNQAFEGSLGQYRDVPQYVDYIKWLSLQDNTEAQAYWQNHLKEVEPCLFPQLTSSPNPINPEGTISAIRATWTRDARMDDLCHKHAITLTNLFQIVWAVVLGAYVGTDEVCFGYTTLGRDVPVDGVETMVGPLVNVLAATVQLKQDESILNALLTHQNRLTSSLQHQHYALADVYACLGLAGSQLFNTIVSLQDISHFDVPDERGTRLEMLPANDVSEYNVALNIGVDKSSIQLVCSYQTVSLSAEQADALLRTVFHVLGEILRDPTQRFCELEVISPKCKEQLVKWNAGMLAPTDEYIHEKIQGQCRIHASRQAVCAWDGMFTYAEVDDLSSRLAARLIRMGVTSEDIIPIYSPKSRWTVIAILGVLKAGAAFTLLETSHPMARLHMICNQIKAPMIIAPASHAVPAANLAPILVVLDNITSLAQEKLDPFPGIGIPPAGEALAYLIFTSGSTGNPKGVMVTHQNLCSNASIITTSVNMTSDSRVLQFASHAFDGCLWEILGALLAGACLIIPSESENKEDLTGCIERMDVTWAFLTPSVARILKPETLPSLCNLVLGGEPIAASDLDMWRGHVQVVCAYGPTETTILASTTSPSTFPTDGKDIGTPTGSSLWIVDTRNYQTMVPLGATGELLIEGPNVSQGYLGDPEKTNDAFPDAPRWLSQLRKSPTRVYRTGDLVRFDTTAGTIRFVGRKDNQIKFHGQRIELGEIEHHAQLAFSSASTVIVDLITPEQPQQPYIVAFVHLPDATPETTETMDTILLPPSESFRADALAAQKKMHKRLPHYMVPAAFLPLHRFPLSATGKADRKRLRQCALGLSSPDLSAYRATASTKRMPSTAAERKMQLLVASVLGRDPTEIGMDDSFFYLGGDSVQAMRLVAEGRQQGLSLSLRAIFDSPRLRDLGDQARSPNADNQRVSTASSAGLRDNRDQIDKVVATNSLKKADVADVLPTTSFQRHWLDAQLKSYIVVDIPGPIDPARLLRAMHRVVEAHPILRVSFVPYETTTLQIILRTAAAMTNVDVSTTTVDFSTTTVEDICRQDAGAQLPPGVPYLRVILATQDKADHKLIMRLSHAQYDAVSLSLLMNDLSHAYATETHALPSSHFPRFNDYITYQQAQRADPTATTFWRLLLQNVSLTYLNLQPAESSASNGTPITLSRDINIATFPSLPNGITIATMVKAAWSLVLAQKTDSHAVIFGQVVHGRTIALPGVEGIVGPCANITPVVARLGLQTTGFELMQTLQDQHRSAMPYEALDLDDALAYTKNSPVGRRGLQTIVQHQNNVMVDDMELLLGEVKCGVDVRAVDHVPKEVWVYSSVDEKRPDMLEVKIMSSTLALGEEVAEELMGLLVEKIVGLLRHPERVCV

mlfA

Malformin synthetase mlfA

5068 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

557.199 kDa

MSRFSCIFPTLTDGYVPNLDQTRAAGRRTYTIDRRGWTAPSSQTESHILAAWGLVLSSYVGTDEVAFYIVPTTGPDTTALAELKVEGDMSRRSLINAAEQLLHPGPVGAGQVSGESANTIITFEKDIESLFVTQAEAANVGTAMAQALAEVGADDHNRLIKNLNLMSPTHLESIWQFNANVPGMWEECFHDVIERRAANRPHSLAVDAWDMKLTYADLVREARLLAAYLQHRGVRPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPTLPAGRLDAILEVIEAPFVVTWSKYEPFWAERLPTLPIDSYPKPSADATVKTLGKPEDLFYVIFTSGSTGRPKGCMLSHSNWLNGALRNAPSWKYGPESRVLQMLSHTFDMSLLEICTSLGSGACVCVPRTEEIETSVSDAINRWQVNHVIMTPSLARSLRRDDVPGLKTMCLGGEAFPREIVTMWSERINLWQFYGPSECSINSSSRPITRPDADPLNIGPPNSAACWVVDTQDYNKLVPVGAIGELLVSGPIVGMGYLKNPIKTAEAFLDEVGFVAKDDPQFGGFRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLEAGVQYAIAMAPQSGRCKNNLIAVLTVKCGGASNQGNADDEIPLLDRHDPIVQQTVKKLRSQLQHALPRYMVPTIWAFVGRMPMSPSGKIDRVQLRNWVQEMSQETFDAITGRSFEAEDHVLGLSQLEQEIQLAWAEALGLSAAEVGLQQPFVALGGDSIKALDAVARCRARQIKISMVHILSCEGVREASSLAEVQETPAHQVAEIAVDYSDLWTRLSTEYDISKLGVTQVEEVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGSLPTVERIQQAWASLVERHPSLRTVFVDDLSSEAIYHSVVLRSVPMELTMREVPRDLNPESALAMFTEELVPFRPNAPLHRMLLLTCRGRVPYLMLEISHVIMDGYALSVFRREFIRACSSTASLPRGPDYRMFANYHRTRQTDESAKYWTNYLADCVPCHIPTDPLSVPTDASPEWPRTLQRRDFGFDNSVAFLQRCKERQVTLACAIRAAWALVLRAYTQSRDVCFGYVSSGRNVPVPEVETIFGLCLSMQVCRAKLSEASTIASLARKIQEDYVASLPFQHYPLAEAQRGLKQTHGQGLFNTAISMEWVPPTAEDEDALLDLEEIREQDDPTEYDIAISVDIHEGHIKLGFLYWPNLSDFQITHLAEALQGAMNCFAFQPDVALNTLTLLQASDLCSTLTNGPTLLPLEAVRGNVISMIDRWVTRQPESPAIDGWDGSLTYKQLHEQSSWVARNLLHQGVKLGDRILVCADRSSRTVVTILGVVRAGCVLVLSNPTDPEKRLQWLAHKCNATLVVADPAYEERFATSGARVFSTTSVCAPAAWDYEFSALDDQDLVSILFTSGSTGTPKGILMDHGALATSVLLGHGRTLRFSRHTRMLHFASLTFDAALAEIFTTLAHGGCICVPCEEDRLSDVSGCISRFAVNTAMLTPSVGRLLDPEALPTLKALAMIGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMKPTDEPVNLGYAVAGVRLWVTEAENPNRLAPLGAVGELIVEGRLVTRGYLDDPARTRESFLPNLPWLPSQHALYRTGDLVRYAEDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQVVVEMVIPEGKIRAQASLVAFVSGLTAADVESSSARNFDQSMPMSQIALPGSTIQALEEALPRYMIPSVYFALDTIPLSVNGKADRRRLREIGAALLVSSTAHKNTVDGKSEPVKWTASSKLELTLLELWTTTLGLEAETIYGDDSFFELGGDSVSAMKLVATARDRFKLSLSVPQMFRHPTIHQLAAILGEATGQPESSASSTTEEGFTFSTPDDSSTNDGVDDDFLRLATAQLAQLAQEKGKKVDIAALLKQLQGGSSSCKTPSVSSSSSSSSSRKKKSAKVVSPVEAPAPVPVPFSLLDGGADVVEKIRAQAVEQCKILPGDIEDIYPATALQEGMMALMARTPGVYTTTLTCELPERVNLARLHSAWDKAAEAHPILRTRIILTENNTAVQVVQRAKELPWDAYSLQDGDPLPDLTSNMTLGSTLLRLAEIHRQNQPRMLLVAIHHALYDGWSMPLLKQAVEDVYHGQELWPQPFTPFINYLNEGKPAAQGYWTAHLDGFAGSVFPNLPSINHHIQPTERRTRSLAVPTAPPGSQYTMATKIQAAWAVTVSRYAEAEDIVFGTVSTGRSAPVPSIDRMVGPTITTVPVRISLGNQAERLTSLLQRVQEDGWNRMDHEHLGLQHIRRLGESAAAACNLQTLLVIQPREEPRAKSISTLLSGLQDVAELKGVDTYPLMLVCEPDGVSLHLTAVFDPAVLDAVMLDRMLAHWELVLNQIWSEPDMAVMGLDGVSYRDKQTLVRWNAGEKIADGCAHDAVYEWSVRTPHAPAVFAWDGKWTYEELEKCSSLIASQVLVHGVSSGDFVALYHEKSRWAAAAILAVFKAGGILVTLDPAHPKDRIKDILDQARPRLVLTSQSLLDEARELETPVMVVQFAASQPMPGECFPLPTVSPTQAAYAPFTSGSTGRPKGIPLEHRGLAASTASVARACLLRPASRVLHFASFAFDASMMEHLIAWHAGSCLCIPVETVRQTDLARCIRDFEVTWAFLTPSCLRLISPDDVQSLEALGLGGESMTPEDIFIWGPRLRQIVQLYGPAECSIVAALTEVTKPSENRLIGRPNACRCWVVDPHSPDRLAPLGAVGELVIEGITVGRGYIDDPERTTQAFIPPPTWIQTLYPNEQQPSRLYRTGDLVRYAGTDGKLTFIGRRDGQLKLHGQRIELADVEAHLRPLIPGTQKVVVEMVHSVGNHHPLLAAFVEEILTSQDQVEQVVNLLHPSQTQCALNVKAIDSALSQTVPQYMIPSMYLHISRLPLSASGKLNRRHLRRLVAEFPRQRLSEYAAGSGLAVPNRPATAQEREMQAIWARVLSVDPDTIGVNEDFFRIGGDSISGMQVATRCNAAGMHITSADLFQHRTIEQLMRHLSANGKTGSASISLPPEPVDEWVPLAPIQQLFFEIAPQGPDHFNQSLLLRTSRRVSAEKLAGGLDILVGRHSMLRARFCRDDSGQWSQQVRSRGPYPASAFYRLTTHNHIAPELLSSLLAASQMALSIQEGPLLAVDLVNLTDDTQLVYLVAHHLIIDLVSWRILHAELEEYLQTGSFASTTGSVPFLTWSRAQAEYSANHLTPTLPLPGFQEANDGFDASRYWGISCESNTFGQTSTSTFTLDQTVTDQLFGPANNVLDTRPAEILQAALWYSFTQSLTDRPGPSIYVEGHGREPWTGSIDLSGTVGWFTTMSPLVSAPWDSLSQTSMRDFLDALSYIKDQRRRIPANGWAYFTSRYLNDEGKVAYGRMKPVVEILFNYMGQYQEMNREDAILQLAGDGIQSGTGAADVADNVPRFSLIDVSAFISNGCLTFQFILPKSLQQDSRLQGCFQEYERTLVAAANSLSTEGPRKTLADFPLMPALTYDQLSQCLDHTLPSMGLCARDVVDIYPCSPVQQGMLLAQLRDRQAYQQRFRFQVKSRGSTDRLTLEKLKDAWTEVINRHDILRTLLLPVSDYSHLDQVVMAPGSLQHLVRINAMDTNPTQGLPHSINITSDSTGTVICEWNVSHALVDAMSIAVIQQEVNEALEGSLGQHQKTPRYADYIQWLSLQDNTETQAYWKKYLEGVEPCLFPKLASSTDKVNPEGTISAIRATWTRDSRLDKLCHTHGITLTNLFHIVWSLLLSAYLGTDKVCFGYTTLGRDVPVDGVEKMVGPLVNVIATTIQLQEDDSILDALLTHQTHLSNSLQHQHYALADVYASLGLVGSQLFNTIVSLQDISHFDANDERPTRVEMLPANDVSEYDVALNIGVDQSSIQVVCSYRTLSLSVEQADALLRTTSHVLDEILRDPKQPLRDLEVISPQCKEQLVKWNAAMPAPTDEYIHEKIQDQCRLHSSREAACAWDGIFTFAEVDDLSSRLAARLIRMGVTSGHIIPIYSPKSRWTVIAILGVLKTGAAFTLLETSHPTARLRVICNEIKADIIIAPASHAVPAATLAPILVVLDSITSMSPQESDLLPAVGMPPAAEALAYLIFTSGSTGNPKGVMVTHQNLCSNASIMTTSVNMTSDSRVLHFASHAFDACLWEIFGALFAGACLIIPSESETKEDLAGCIERMVVTWAFLTPSVARILKPEALPSLRNLVLGGEPIAASDLDMWRGHVQVVCAYGPTETAILASTTSPSTFPSDGKDIGVPTGSSLWIVDKQNYNKLAPHGATGELLIEGPNVSQGYLGDPEKTNEAFPVAPRWLSQLRKSPTRVYRTGDLVRFNTSTGTIHFVGRKDNQIKFHGQRIELGDIEHHAQQAFSNASMVIVDLITPEQPQQPYIVAFVHQADTRTGTADPIDTILLPPSESFRADAIGAQNHMHKRLPHYMVPTAFLPLHRLPLSGTGKADRKRLRQCALSLSSLELNAYRATASAKRMPFTAAECKMQELVATVLGRDMSEIGMDDSFFYLGGDSIQAMRLVSEGRQQGLTLSLQAIFDAPRLGDLAYRTANLVRVSEPAPPTLPATSSDDCDHKETIVAALPIKKTDVAEVLPTTSFQRTWLDSQLKSYIVVDIPGPIDLARLRTAIQRVVKAHPILRASFVPYETTTMQVILRTAVVITEADLSTTTVEGICRKDANAPMAPGTPYLRVILATQGEVDRKLIMRLSHAQYDGISLSLLMNDLSHAYASESRPLPSSHLPAFNDYITYQQTQGADPTATTFWHRLLKDVPITYLDLQPAETPTSNGSLITRTRDINIAAFPSLPNGITTATAVKAAWSLVLAQKTGSLAVIFGQVVHGRGIALTGVEGIVGPCANITPVVARLGPQTTRMELMQTLQDQHRSAMPYETVSLDDALAYTKDSQAGRKELQTIVQHQNNVMADDMELSLGEARCRVDLRAVDHVPQEVWVYSSIDGGRPGMLEVKIMSSTLVLSEEVAEELMDLLVEMMKRLFSDPEGVCV

mlfA

Malformin synthetase mlfA

5054 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

555.691 kDa

MSRFSCIFPTLTDGYVPNLDQTRAAGRRTYTIDRRGWTAPSSQTESHILAAWGLVLSSYVGTDEVAFYIVPTTGPDTTALAELKVEGDMSRRSLINAAEQLLHPGPVGAGQVSGESANTIITFEKDIESLFVTQAEAANVGTAMAQALAEVGADDHNRLIKNLNLMSPTHLESIWQFNANVPGMWEECFHDVIERRAANRPHSLAVDAWDMKLTYADLVREARLLAAYLQHRGVRPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPTLPAGRLDAILEVIEAPFVVTWSKYEPFWAERLPTLPIDSYPKPSADATVKTLGKPEDLFYVIFTSGSTGRPKGCMLSHSNWLNGALRNAPSWKYGPESRVLQMLSHTFDMSLLEICTSLGSGACVCVPRTEEIETSVSDAINRWQVNHVIMTPSLARSLRRDDVPGLKTMCLGGEAFPREIVTMWSERINLWQFYGPSECSINSSSRPITRPDADPLNIGPPNSAACWVVDTQDYNKLVPVGAIGELLVSGPIVGMGYLKNPIKTAEAFLDEVGFVAKDDPQFGGFRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLEAGVQYAIAMAPQSGRCKNNLIAVLTVKCGGASNQGNADDEIPLLDRHDPIVQQTVKKLRSQLQHALPRYMVPTIWAFVGRMPMSPSGKIDRVQLRNWVQEMSQETFDAITGRSFEAEDHVLGLSQLEQEIQLAWAEALGLSAAEVGLQQPFVALGGDSIKALDAVARCRARQIKISMVHILSCEGVREASSLAEVQETPAHQVAEIAVDYSDLWTRLSTEYDISKLGVTQVEEVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGSLPTVERIQQAWASLVERHPSLRTVFVDDLSSEAIYHSVVLRSVPMELTMREVPRDLNPESALAMFTEELVPFRPNAPLHRMLLLTCRGRVPYLMLEISHVIMDGYALSVFRREFIRACSSTASLPRGPDYRMFANYHRTRQTDESAKYWTNYLADCVPCHIPTDPLSVPTDASPEWPRTLQRRDFGFDNSVAFLQRCKERQVTLACAIRAAWALVLRAYTQSRDVCFGYVSSGRNVPVPEVETIFGLCLSMQVCRAKLSEASTIASLARKIQEDYVASLPFQHYPLAEAQRGLKQTHGQGLFNTAISMEWVPPTAEDEDALLDLEEIREQDDPTEYDIAISVDIHEGHIKLGFLYWPNLSDFQITHLAEALQGAMNCFAFQPDVALNTLTLLQASDLCSTLTNGPTLLPLEAVRGNVISMIDRWVTRQPESPAIDGWDGSLTYKQLHEQSSWVARNLLHQGVKLGDRILVCADRSSRTVVTILGVVRAGCVLVLSNPTDPEKRLQWLAHKCNATLVVADPAYEERFATSGARVFSTTSVCAPAAWDYEFSALDDQDLVSILFTSGSTGTPKGILMDHGALATSVLLGHGRTLRFSRHTRMLHFASLTFDAALAEIFTTLAHGGCICVPCEEDRLSDVSGCISRFAVNTAMLTPSVGRLLDPEALPTLKALAMIGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMKPTDEPVNLGYAVAGVRLWVTEAENPNRLAPLGAVGELIVEGRLVTRGYLDDPARTRESFLPNLPWLPSQHALYRTGDLVRYAEDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQVVVEMVIPEGKIRAQASLVAFVSGLTAADVESSSARNFDQSMPMSQIALPGSTIQALEEALPRYMIPSVYFALDTIPLSVNGKADRRRLREIGAALLVSSTAHKNTVDGKSEPVKWTASSKLELTLLELWTTTLGLEAETIYGDDSFFELGGDSVSAMKLVATARDRFKLSLSVPQMFRHPTIHQLAAILGEATGQPESSASSTTEEGFTFSTPDDSSTNDGVDDDFLRLATAQLAQLAQEKGKKVDIAALLKQLQGGSSSCKTPSVSSSSSSSSSRKKKSAKVVSPVEAPAPVPVPFSLLDGGADVVEKIRAQAVEQCKILPGDIEDIYPATALQEGMMALMARTPGVYTTTLTCELPERVNLARLHSAWDKAAEAHPILRTRIILTENNTAVQVVQRAKELPWDAYSLQDGDPLPDLTSNMTLGSTLLRLAEIHRQNQPRMLLVAIHHALYDGWSMPLLKQAVEDVYHGQELWPQPFTPFINYLNEGKPAAQGYWTAHLDGFAGSVFPNLPSINHHIQPTERRTRSLAVPTAPPGSQYTMATKIQAAWAVTVSRYAEAEDIVFGTVSTGRSAPVPSIDRMVGPTITTVPVRISLGNQAERLTSLLQRVQEDGWNRMDHEHLGLQHIRRLGESAAAACNLQTLLVIQPREEPRAKSISTLLSGLQDVAELKGVDTYPLMLVCEPDGVSLHLTAVFDPAVLDAVMLDRMLAHWELVLNQIWSEPDMAVMGLDGVSYRDKQTLVRWNAGEKIADGCAHDAVYEWSVRTPHAPAVFAWDGKWTYEELEKCSSLIASQVLVHGVSSGDFVALYHEKSRWAAAAILAVFKAGGILVTLDPAHPKDRIKDILDQARPRLVLTSQSLLDEARELETPVMVVQFAASQPMPGECFPLPTVSPTQAAYAPFTSGSTGRPKGIPLEHRGLAASTASVARACLLRPASRVLHFASFAFDASMMEHLIAWHAGSCLCIPVETVRQTDLARCIRDFEVTWAFLTPSCLRLISPDDVQSLEALGLGGESMTPEDIFIWGPRLRQIVQLYGPAECSIVAALTEVTKPSENRLIGRPNACRCWVVDPHSPDRLAPLGAVGELVIEGITVGRGYIDDPERTTQAFIPPPTWIQTLYPNEQQPSRLYRTGDLVRYAGTDGKLTFIGRRDGQLKLHGQRIELADVEAHLRPLIPGTQKVVVEMVHSVGNHHPLLAAFVEEILTSQDQVEQVVNLLHPSQTQCALNVKAIDSALSQTVPQYMIPSMYLHISRLPLSASGKLNRRHLRRLVAEFPRQRLSEYAAGSGLAVPNRPATAQEREMQAIWARVLSVDPDTIGVNEDFFRIGGDSISGMQVATRCNAAGMHITSADLFQHRTIEQLMRHLSANGKTGSASISLPPEPVDEWVPLAPIQQLFFEIAPQGPDHFNQSLLLRTSRRVSAEKLAGGLDILVGRHSMLRARFCRDDSGQWSQQVRSRGPYPASAFYRLTTHNHIAPELLSSLLAASQMALSIQEGPLLAVDLVNLTDDTQLVYLVAHHLIIDLVSWRILHAELEEYLQTGSFASTTGSVPFLTWSRAQAEYSANHLTPTLPLPGFQEANDGFDASRYWGISCESNTFGQTSTSTFTLDQTVTDQLFGPANNVLDTRPAEILQAALWYSFTQSLTDRPGPSIYVEGHGREPWTGSIDLSGTVGWFTTMSPLVSAPWDSLSQTSMRDFLDALSYIKDQRRRIPANGWAYFTSRYLNDEGKVAYGRMKPVVEILFNYMGQYQEMNREDAILQLAGDGIQSGTGAADVADNVPRFSLIDVSAFISNGCLTFQFILPKSLQQDSRLQGCFQEYERTLVAAANSLSTEGPRKTLADFPLMPALTYDQLSQCLDHTLPSMGLCARDVVDIYPCSPVQQGMLLAQLRDRQAYQQRFRFQVKSRGSTDRLTLEKLKDAWTEVINRHDILRTLLLPVSDYSHLDQVVMAPGSLQHLVRINAMDTNPTQGLPHSINITSDSTGTVICEWNVSHALVDAMSIAVIQQEVNEALEGSLGQHQKTPRYADYIQWLSLQDNTETQAYWKKYLEGVEPCLFPKLASSTDKVNPEGTISAIRATWTRDSRLDKLCHTHGITLTNLFHIVWSLLLSAYLGTDKVCFGYTTLGRDVPVDGVEKMVGPLVNVIATTIQLQEDDSILDALLTHQTHLSNSLQHQHYALADVYASLGLVGSQLFNTIVSLQDISHFDANDERPTRVEMLPANDVSEYDVALNIGVDQSSIQVVCSYRTLSLSVEQADALLRTTSHVLDEILRDPKQPLRDLEVISPQCKEQLVKWNAAMPAPTDEYIHEKIQDQCRLHSSREAACAWDGIFTFAEVDDLSSRLAARLIRMGVTSGHIIPIYSPKSRWTVIAILGVLKTGAAFTLLETSHPTARLRVICNEIKADIIIAPASHAVPAATLAPILVVLDSITSMSPQESDLLPAVGMPPAAEALAYLIFTSGSTGNPKGVMVTHQNLCSNASIMTTSVNMTSDSRVLHFASHAFDACLWEIFGALFAGACLIIPSESETKEDLAGCIERMVVTWAFLTPSVARILKPEALPSLRNLVLGGEPIAASDLDMWRGHVQVVCAYGPTETAILASTTSPSTFPSDGKDIGVPTGSSLWIVDKQNYNKLAPHGATGELLIEGPNVSQGYLGDPEKTNEAFPVAPRWLSQLRKSPTRVYRTGDLVRFNTSTGTIHFVGRKDNQIKFHGQRIELGDIEHHAQQAFSNASMVIVDLITPEQPQQPYIVAFVHQADTRTGTADPIDTILLPPSESFRADAIGAQNHMHKRLPHYMVPTAFLPLHRLPLSGTGKADRKRLRQCALSLSSLELNAYRATASAKRMPFTAAECKMQELVATVLGRDMSEIGMDDSFFYLGGDSIQAMRLVSEGRQQGLTLSLQAIFDAPRLGDLAYRTANLVRVSEPAPPTLPATSSDDCDHKETIVAALPIKKTDVAEVLPTTSFQRTWLDSQLKSYIVVDIPGPIDLARLRTAIQRVVKAHPILRASFVPYETTTMQVILRTAVVITKADLSTTTVEGICRKDANAPMAPGTPYLRVILATQGEVDRKLIMRLSHAQYDGISLSLLMNDLSHAYASESRPLPSSHLPAFNDYITYQQTQGADPTATTFWHRLLKDVPITYLDLQPAETPTSNGSLITRTRDINIAAFPSLPNGITTATAVKAAWSLVLAQKTGSLAVIFGQVVHGRGIALTGVEGIVGPCANITPVVARLGPQTTRMELMQTLQDQHRSAMPYETAGRKELQTIVQHQNNVMADDMELSLGEARCRVDLRAVDHVPQEVWVYSSIDGGRPGMLEVKIMSSTLVLSEEVAEELMDLLVEMMKRLFSDPEGVCV

mlfA

Malformin synthetase mlfA

5044 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

555.887 kDa

MSRFSCIFPTLTDGYLGTDQVAFYVVPTTGPDTTALAELKVEGDMSRQSLNYAANHLLHPGLVGAGQVSGETANTIVTFADDIESLFVTQTEESFLSLHVYRDEQGHISLSLTYYLSLLTDAQATNVGTALVQALAEVGTCDNDRLIKDLNLMSPAHLEHIWRFNADVPGIWEECFHDVIERHAANRPHSLAVDAWDTKLTYTDLVREARLLAAYLQQRGVGPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPNLPAGRLDAILEVIEAPFVVTWTKYESFWAERLPTLPIDNYPKPAADATVEALGKPEDLFYVIFTSGSTGRPKGCMLSHTNWLNGALRSAPNWKYGPESRVLQMLSHTFDMSLLEICTSLGSGSCVCVPRAEEIETSISDAINRWQVNHVIMTPSLARALRPDDVPGLKTMCLGGEAFPKEIVTMWSERINLWQFYGPSECSINSSSRPITRPDADPLNIGPPNSAACWVVDVHDYNKLVPVGAIGELLVSGPIVGMGYLKNPVKTAEAFLDEVGFVAKDDPQFGGLRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLEAGVQYAIAMAPQAGLCKNNLIAILTVKGTSTGNQDTAADEIPLLDRRDPVVQETVKKLRSQLQHALPRYMVPTIWAFVGRMPMSASGKIDRVQLRDWVQKMSQETFDAITGRSLEAEDHVLGLSRLERDIQLAWAEALGLSAAEVGLQQPFVALGGDSIKALDAVARCRARQIKISMVHILSCEGVREAASLAEVQITPPQQVAEMAVDYSNLWTRLSYDYDLDKLGVTQVEEVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGCLPTVERIQQAWASLVERHPSLRTVFVDDLSPEAIYHSIVLRSVPMELRMREVPRDLSAEAALAMFTEELVPFPANAPLHRMLLLTCRGRVLYFMLEISHVIMDGYALSVFRREFIRACSSSTPLPRGPDYRMFVNYNRTRQTDDSARYWTNYLADCVPCHIPTHAVSAPSDDPPEWPRTLQRRDFGFDNSAAFLQRCKERQVTLACAIRAAWALVLRAYTQSEDVCFGYVSSGRNVPVPEVETIFGLCLSMQVCRARLSEASTIASLARKIQEDYVASLPFQHYPLAEAQRGLKQAHGQGLFNTAISMEWVPPSVEDEDALLDLEEIREQDDPTEYDIAISVDVHEGHIKLGFLYWPNLTDFDIAHLAEALRGAMNRFAFQPDEALNTLSLLQASDVCSALAGGPTLLPLEAVRGNVVSMIDRWVTRQPEGAAIDGWDGSLSYKGLHEQSSWVARNLLHQGVQLGDRVLVCADRSSRTVATVLGIVRAGCVLVLSNPTDPEKRLQWLANKCNAALIVADPTYEERFATAGARVLSTTSVCAPAAWDYEFPSLDEHDLVSILFTSGSTGTPKGILMEHGALATSVLLGHGRTLRFSRHTRMLHFASLTFDAALAEMFTTLAHGGCICVPYEEDRLSDVPGCISRFAVNTAMLTPSVGRLLDPGALPTLKTLIMVGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMKPTDEPVNLGYAVAGVRLWVTEAENPNRLAPLGAVGELIVEGRLVTRGYLDDPARTQKAFLPSLPWLPSQHALYRTGDLVRYADDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQIVVEMVVPAGKMRAQASLVAFVSGLTAADVESSSACNLEGTIPISQIVLPKSAFQALEEVLPRHMIPSVYYALDTIPLSVNGKVDRRRLREMGTLLLASSAAHKNIIEGMSKSVKWTPASELERTLLELWAATPGLEADTIHGDDSFLELGGDSVSAMKLVATARDKYKLSLSVPQMFRYPTICQLAAEVGGPAGQSASSASSTTEEGFTFSTPDDSSTNDGVDDDFLQLAAAQLAQLAQDKGKKVDIAALLKQLQGGSSSNKTLSVSFSSSSSSSSKRKKKAALAEAAAPIPVQFSLLDGGADVLDKVRAQAVEHCKIPHEDIEDIYPATALQEGMMALTARTPGVYTTTLTCDLSEQVNLARLHYAWGKAAEAHPILRTRIILTDNNMAVQVVQRAKGLPWDTYSLREGDVLPDLTSNMTSGSPLLRLAVVHRQSQPRMFLVAIHHALYDGWSMPLLKQAVEDAYHGRDLRPQLFTLFIKHLIAGKPAAQDFWTTHLDSFAGGVFPNLPSVDHQVQPKERRARSLTLPTATPRSQYSMATKIQAAWALTVSRYAEANDIVFGTVSTGRSAPVPAIDRMVGPTITTVPVRISLGDQTERVISILQRVQENSWNIMDHEHLGLQHIRRLGESAAAACSFQTLLVIQPREQTDTKYRYTLLSGLQDVAEFEGVDTYPLMLVCEPDGARLHLTAVFDPAVLDGATLDRMLAHWELVLTQLWNESDMAVIELDTVSCTDKETLMRWNTGQTIPNACAHDAVYEWSVHTPHAPAVCAWDGEWTYEEVERCSSLIAGHILAHGVSCGDSVALYHEKSRWGAAGILAVFKAGAILIALDPTHPTDRIKDILDQARPRLILTSQFLLDEARNLETSVLSVQFAASQPLPEGCSPLPKINSTQAAYIPFTSGSTGRPKGIPLDHRGLAASTASIARSCLLSPASRVLHFASFAFDASMMEHLIAWHAGGCLCIPDETARQTDLAKCIRDFNVTWAFLTPSCLRLITPDDVPSLQALGLGGESMTSEDIIIWGPRLRQIIQLYGPAECCFVAALNEVTKPSENRLIGRPNACRCWVVDPRSPDRLAPIGAVGELIIEGITVGRGYINSPDRTTRAFIRPPNWLQTLYPDDHEPKRLYRTGDLVRYAGVDGKLAFIGRRDSQLKLHGQRIELADVEAHLRPLIHGTQKMVVEMVHSADNQNPILAAFLEEMSTSQNSAEREVGLLHPSQSQCALDVMAIHSALSRTVPHYMIPSMYLHISRLPLSASGKLNRRHLREMVAELPRQRLNEYAAGSSLSVPDRPKTSQEQEMQAIWARVLSLDPNTIGVNEDFFRIGGDSISGMQVATKCNAAGIHITSADIFRYRTIEQLICHLNFIRITDCASVLLPAEPVDEWVALAPIQQLFFEVAPEGPNHFNQSLLLRTSRRVSVGELAGGLDILVGRHSMLRARFCRKDSGQWFQQVRSLDSEPASAFYRLAAHNQITRESLPTLFTAAQMALSIEDGPLLTVDLVELEDGNQLVYLAAHHLIIDFVSWRILHGDLEEYFQTGSLSSATGSVPFLTWTQLQAEYSAEHLPPLNDDFDVMRYWGISSESNTFGQTSISRFTLDRTVTDILFGSANNVIDTRPVEILQAALWYSCNQALTDHPGPSIYVESHGREPWTNSINVSGTVGWFTTMSPLVSTPWDHLSRKSMRDFVDVLSYIKDQRRRIPANGWAYFTSRYLNDEGRVAYGRTKPVVEVLFNYMGQYQEMKRDDAMLQLAGDDIQSGTGASDIADNVPRFSLIDVTAFTANGCLTFEFTFPQLMQQDARLEQCIKECERTLVAAASSLPAEGPRKTLTDFPLMSALTYDQLSQCLNYTLPSMGLRAQDVWNIYPCCPVQQGMLLAQLRDRQAYQQRFRFQVMSRGPTDQLPLEKVKDAWTEVINRHEILRTLLRPVSDHSHFDQVVMVPGSLQHLVRINAMNDNPTEGLPHTINITSDSTGTTICEWNVSHALVDAMSIAVIQREVNQVFEGALGQHRDVPQYVDYIQWLSLQDNTEAQAYWQNYLNGVEPCLFPKLTSSPDKVDSEGTISAIRATWSRDARMDELCLRHAITLTNLFHIVWAIVLGAYVGTDEVCFGYTALGRDVPVNRVETMVGPLVNVLATTVRHQEDETILNALLVHQAHLTNSLQHQHYALADVYASLGLVGSQLFNTIVSLQDTSHFDAPDEQRARLEMLPANDVSEYDVALNIGVDKSSIQLVCSYQSVSLSAEQADALLRTAFHVLGEILRDPTQRFCELEVISPKCKEQLVRWNAGMLAPTDEYIHEKIQGQCRIHNSRQAVCAWDGMFTYAEVDDLSSRLAARLIRIGVTFEDIIPIYSPKSRWTVIAILGVLKAGAAFTLLETSHPMARLHVICDQIKAPMIIAPASHAVPAANLIPILVVLDNITSLTQEKSDLLPAVGIPPAGEALAYLIFTSGSTGNPKGVMVTHQNLCSNASIITTSVNMTSDSRVLQFASHAFDGCILELLGALIAGSCLIIPSESENKEDLAGCIERMDVTWALLTPSVARILEPETLPRLRNLVLGGEPIAASDLDMWRGHVQVVCAYGPTETTIIASTTSPSTFPTDGKDIGLPNGSSIWVVSRQNYQKLAPLGATGELLIEGPNVSLGYLGDPEKTKEAFPDSPRWLSQLRKSPPRVYRTGDLVRFDTTTGTIRFVGRKDNQIKFHGQRIELGEIEHHAQLAFPSASMVIVDLITPEQPQQPYIVAFVHQSDATNETTDTTDSLLLPPSEAFRADALAAQNKMHERLPHYMVPAVFLPLHRLPLSVTGKADRKHLRQCALALSSPELNTYRATASTKHTPSTAEERKMQELVATVLGRDPTEIGMDDSFFYLGGDSVQAMRLVAEGRQQGLALSLRAIFDSPRLRDLADQARSPNADNQRVSTASSAGPRDDCDQIDRIVATNSLNKADVADVLPTTSFQRHWLEAQLKSYIVVDISGPVDPARLLRAMHRVVEAHPILRVSFVPYETTTLQVILRTAVAITNADLSTTTVEEFCRRDVDAQMAPGVPYLRVILATQDKADHKLIMRLSHAQYDAVSLSLLMNDLSHAYANEIHPLPSTHFPRFNDYITYQQAQRADPTATTFWRHLLQDVSLTYLNLQPAESSASNGTPIILSRDINIAAFPSLPSDITIATTIKAAWSLVLAQKTDFLAVIFGQVVHGRAIALPGVEGIVGPCANVTPVVARLGLQTTGMELMQTLQDQHRSAMPYETVDLDDALAYAKDSQAGRKGLQTIVQHQNNVMVDDVELSLGEVKCGVDARAVDHLPKEVWVYSSVDENRPGMLEVKIMSSTLVMGEEVAKELMGLLVEKIVGLLRHPEIVCV

mlfA

Malformin synthetase mlfA

4960 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

545.55 kDa

MSRFSCIFPTLTDGYVSNPDHTRAAGRRTYTIDLSGWKAPGSETEAHILAAWGLVLSSYVGTDEVAFYVVPTTGPDTTALADLKVEGDMSRQSLTYAAEQLLHPGLVGAGQVSGETANTIITFAKDIESLFVTQTEAANVGTAMAQALAEVGTCDNDRLIKDLNLMSPAHLEHIWRFNANVPGIWEESFHDVIERHATNRPHSPAVDAWDTKLTYADLVREARLLAAYLQQRGVGPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPNLPAGRLDAILDVIEAPFVVTWTRYESFWAERLPTLPIDNYPKPAADTTVEALGKPEDLFYVIFTSGSTGRPKGCMLSHSNWLNGALRNAPSWKYGPESRVLQMLSHTFDMSLLEICTSLGSGACVNHVIMTPSLARALRPDDVPELKTMCLGGEAFPKEIVTMWSERINLWQFYGPSECSINSSSRPITRPDADPLNIGPPNSAACWVADVHNYNKLVPVGAIGELLVSGPIVGMGYMKNPVKTAEAFLEEVGFVAKDDPQFGGFRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLESGVQYAIAMAPQAGLCKNNLIAILTVKGTSTGNQDTAADEIPLLDRRDPIVQETVKKLRSQLQHALPRYMVPTIWAFVGRMPMSASGKIDRVQLRDWVQKMNQETFDAITGRSLEAEDHVLGLSRLEQEVQLAWAEALSLSAAEVGLQQPFVALGGDSIKALDAVARCRARQIKISMVHTLSCEGVREAASLAEVQETPAQQVAEMAVDYSNLWTRLSDDYDLDKLGVTQLEEVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGCLPTVERIQQAWASLVERHPSLRTVFVDDLSPEAIYHSIVLRSVPMELRMREVPRDLRAEAALAMFTEELVPFRANAPLHRMLLLTCRGRVPYFMLEISHVIMDGYALSVFRREFIRACSSSAPLPRGPDYRMFANYHRTRQTDDSARYWTNYLADCVPCHIPTHAVSAPSDAPPEWPRTLQRRDFGFENSAAFLQRCKERQVTLACAIRAAWALVLRAYTQSEDVCFGYVSSGRNVPVPEVETIFGLCLSMQVCRARLSEASTIASLARKIQEDYAASLPFQHYPLAEAQRGLKQTHGQGLFNTAISMEWVPPSVEDEDALLDLEEICEQDDPTEYDIAISVDVHEGHIKLGFLYWPNLTDFEITHLAEALRGAMNRFAFQPDEALNTLSLLQASDVCSALADGPTLLPLEAVRGNVVSMIDRWVTRQPEGAAIDGWDGSLSYKELHEQSSWVARNLLHQGVQLGDRVLVSCEPDACWCSRTRRTQRSASNGWQKKCNAALIVADPTYEERFATAGARVLSTTTVCAPAAWDYEFPSLDEHDLVSILFTSGSTGTPKGILMEHGALATSVLLGHGRTLRFSRYTRMLHFASLTFDAALAEMFTTLAHGGCICVPCEDDRLSDVSGCISRFAVNTAMLTPSVGRLLDPGALLTLKTLIMVGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMEPTDEPVNLGYAVAGVRLWVTEAENPNRLAPLGAVGELIVEGRLVTRGYLDGPARTQEAFLPSLPWLPSQHALYRTGDLVRYADDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQIVVEMVVPAGKMRAQASLVAFVSGLTAEDVESSSACNLEGTILISQIVLPKSAFQALEEVLPRHMIPSVYNALDTIPLSVNGKADRRRLLPPIRTPLKESKSVKWTPASELERTLLELWAATLGLEAETIHGDDSFFELGGDFVSAMKLVATARDKFKLSLSVPQMFRYPTICHLAAEVGEPAGQSASSASSTTEEGFTFSTPDDSSTNDGVDDDFLELVTAQLAQLAQEKGKKVDIAALLKQLQGGSSSNKTPSVSSSSSSSSSSKRKKNAAKAESLAEAAAPIPVQFSLLDGGADALDKVRAQAVEHCKITHEDIEDIYPATALQEGMMALTARTPGVYTTSLTGNLSELVDLAWLQYAWGKAAEAHPILRTRIILTDNNTAVQVVQRAKGLPWDTYSLREDDVLPDLTSNMTSGSPLLRLALVHRQNQPRMLLVAIHHALYDGWSMPLLKQAVEDAYHGRDLRSQPFTPFIKHLIAGKPAAQDFWTTHLDSFVGGIFPKLPSIYHQIQPTKRRTRPMTLPTAAPKAQYTMATKIQAAWAVTVPRYAEVNDIVFGTVSTGRSAPVPAIDRMVGPTITTVPVRISLGDQADRVLSLLQRVQEDSWNKLDHEHLGLQHIRHLGESAAAACSFQTLLVILPREQPDTKYRSTLLSGLQDVAELEGVDTYPLMLVCEPDSARLHLTAVFDRAVLDGATLDRMLAHWELVLTQMWNEPDMAVIEIDAVSCSDKETLMRWNTGETIPDGCAHDAVCEWSRRTPHAPAVCAWDGDWTYEELERCSSLVASQIFAHGLSSGDFVALYHEKSRWTAAGILALFKAGAILITLDPAHPTDRIKDILDQARPRLILTSQSLLDVARNLETPVLSVQLAASQPLPEGWSSLPTISPTLAAYAPFTSGSTGRPKGIPLDHRGLAASTASIARSCLLRPASRVLHFASFAFDASMMEQLIAWHAGGCLCIPDETARQTDLAKCIRDFNVTWAFLAPSCLRLITPDDVPSLQALGLGGESMTSEDITIWSPRLRQIIQLYGPAECCIVAALTEVTKPSENRLIGRPNACRCWVVDPQNPDRLAPIGAVGELLIEGITVGRGYINDPDRTTPAFIRPPKWLQTLYPDDQEPKRLYRTGDLVRYAGVDGKLAFIGRRDGQLKLHGQRTELADVEAHLRSLIPGMQKMVVEMVHSADNQSPFLAAFLEEISTSQKPKEREIGLLHLSQSQCALDVKAIDSALSRTVPQYMIPSMYLHISRLPLSASGKLDRRHLREMVAELPHQRINEYAAGSGLSVPDRPVTSQEREMQAIWARVLSLDPNTIGVNDDFFRIGGDSISGMQVSTKCNAAGIHITSADLFRHRTIEQLICHINTIRTTDCASVSLPTEPVDEWVALAPIQQLFFEVAPEGPNHFNQSLLLRTSRRVGVEELAGGLDILIGRYSMLRARFCRKDSGQWFQQVKSLDSEPVSAFYRLAAHNQITRESLPTLFTAAQMALSIEDGPLLTVDLVELKDGSQLVYLAAHHLIIDLVSWRILHGDLEEYLQTGSLSSATGSVPFLTWTQLQAEYSAEHLTPARALPGFQEANDDFDVMRYWGISSESNTFGQTSTSRFTLDRTVTDILFGSANKVMDTRPVEILQAALWYSCNQALTDHPGPRIYVEGHGREPWTDSIDVSGTVGWFTIMSPLVSTPWHHLSRKSMRDFVDVLSYIKDQRRRIPANGWAYFTSHYLNDEGRVAYGRTKPVMEVLFNYMGQYQEMKREDAMLQLAGDDVQSGTGASDIADNVPRFSLIDVTAFTANGCLTFEFTFPQLIQQDARLEHCIKESFDGPTCPRRLEYLPLLSRATGHVVGAAARSAGISATIQVSGYVSRANRPTFIGKVSDHSHFDQVVMVPGSLQHLVRGDAMDANPTEGLPHTINITSDSTGAIICEWNVSHALVDAMSIAVIQREVNQALEGSLGQHQNLPQYVEYIEWLTLQDNTEAQAYWQKYLDGVEPCLFPKLTSSPDKVNPEATIAAIRATWSRDARMDELCHKHAITLTNLFHIVWAIVLGAYVGTDEVCFGYTALGRDVPVHRVETMVGPLVNVLATTVRHEENETILNALLTHQAHLTNSLQHQHYALADVYAALGLVGSQLFNAIVSLQDTSHFDAPDEQRTRLEMLPANDVSEYDVALNIGVDKSSIQLVRSYQTVSLSAEQADALLRTAFHVLDEILRDPTQRFCELEVISPKCKEQLVKWNAGMLAPTDEYIHEKIQGQCRIHNSRQAVCAWDGIFTYAEVDDLSSRLAARLIRMGVTSEDIIPIYSPKSRWTVIAILGVLKAGAAFTLLETSHPMARLHVICNQIKAPMIIAPASHAIPAANLAPILVVLDKIMSLAQERPVPLPAVGIPPAREALAYLIFTSGSTGNPKVVMVTHQNLCSNASIITTSVNMTSDSRVLQFASHAFDACILGLLGALIAGACLIIPSESENKEDLAGCMERMDVTWALLTPSVARILKPETLPRLLNLVLGGEPIAASDLDMWRGHVQVVCAYGPTETTIVASTTSPSTFPMDGKNIGVPSGSSLWVVSRQNYQKLAPLGATGELLIEGPNVSLGYLGDPGKANKAFPDSPIWLSQLRKSPTRVYRTGDLVRFDTTTGTIRFVGRKDNQIKFHGQRIELGEIEHHAQLAFSSASMVIVDLITPEQPQQPYIVAFVHQSDAANETTDTNDTLLLPPSEAFRADALAAQNKMYERLPHYMVPAVFLPLHRLPLSVTGKADRKRLRQCALALSSPELSAYRATASRKRMPSTAAERKMQGLVATVLGRDPTEIGMDDSFFYLGGDSVQAMRLVAEGRQQGLTLSLRAIFDSPCLGDLSDQAKSLIEDNQRASTASRGNLRYDCDQIDKIVATKSLNKTDVVDVLPTTSFQHHWLDAQLKSYIVADISGPIDPARLLRAMHRVVEAHPILRVSFVPYENTTMQVILNKAVAIKSADPSNTTVEEICRQDADTPTVPGMPYLRVILATQVEADHTLILRLSHAQYDAVSLSLLMNDLGHAYANETHPLPSSHFPRFNDYTTYQQAQRADPTAITFWRHLLQDVSLTYLNLQPAESSASNGTPITLSRDIDIAIFPSLPSDITIATTVKAAWSLVLAQKTNSPAVIFGQVVHGRAIALPGVEGIIGPCANITPVVARLGLQTTGLELMQTLQDQHRSAMPYETVDLDDALAYAKDSQAGRKGLQTIVQHQNNVMVDDMELSLGEVKCGVDVRAVDHLPKEVWVYSSVDEKRPGMLEVKIMSSTLVLGEEVAEELMGLLVEKIVGLLRHPESVCF

mlfA

Malformin synthetase mlfA

4870 amino acids

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties (PubMed:30560908). The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additionnal tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).

Belongs to the NRP synthase family.

535.293 kDa

MSRRSLINAAEQLLHPGPVGAGQVSGESANTIITFEKDIESLFVTQAEAANVGTAMAQALAEVGADDHNRLIKNLNLMSPTHLESIWQFNANVPGMWEECFHDVIERRAANRPHSLAVDAWDMKLTYADLVREARLLAAYLQHRGVRPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPTLPAGRLDAILEVIEAPFVVTWSKYEPFWAERLPTLPIDSYPKPSADATVKTLGKPEDLFYVIFTSGSTGRPKGCMLSHSNWLNGALRNAPSWKYGPESRVLQMLSHTFDMSLLEICTSLGSGACVCVPRTEEIETSVSDAINRWQVNHVIMTPSLARSLRRDDVPGLKTMCLGGEAFPREIVTMWSERINLWQFYGPSECSINSSSRPITRPDADPLNIGPPNSAACWVVDTQDYNKLVPVGAIGELLVSGPIVGMGYLKNPIKTAEAFLDEVGFVAKDDPQFGGFRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLEAGVQYAIAMAPQSGRCKNNLIAVLTVKCGGASNQGNADDEIPLLDRHDPIVQQTVKKLRSQLQHALPRYMVPTIWAFVGRMPMSPSGKIDRVQLRNWVQEMSQETFDAITGRSFEAEDHVLGLSQLEQEIQLAWAEALGLSAAEVGLQQPFVALGGDSIKALDAVARCRARQIKISMVHILSCEGVREASSLAEVQETPAHQVAEIAVDYSDLWTRLSTEYDISKLGVTQVEEVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGSLPTVERIQQAWASLVERHPSLRTVFVDDLSSEAIYHSVVLRSVPMELTMREVPRDLNPESALAMFTEELVPFRPNAPLHRMLLLTCRGRVPYLMLEISHVIMDGYALSVFRREFIRACSSTASLPRGPDYRMFANYHRTRQTDESAKYWTNYLADCVPCHIPTDPLSVPTDASPEWPRTLQRRDFGFDNSVAFLQRCKERQVTLACAIRAAWALVLRAYTQSRDVCFGYVSSGRNVPVPEVETIFGLCLSMQGLFNTAISMEWVPPTAEDEDALLDLEEIREQDDPTEYDIAISVDIHEGHIKLGFLYWPNLSDFQITHLAEALQGAMNCFAFQPDVALNTLTLLQASDLCSTLTNGPTLLPLEAVRGNVISMIDRWVTRQPESPAIDGWDGSLTYKQLHEQSSWVARNLLHQGVKLGDRILVCADRSSRTVVTILGVVRAGCVLVLSNPTDPEKRLQWLAHKCNATLVVVDPAYEERFATSGARVFSTTSVCAPAAWDYEFSALDDQDLVSILFTSGSTGTPKGILMDHGALATSVLLGHGRTLRFSRHTRMLHFASLTFDAALAEIFTTLAHGGCICVPCEEDRLSDVSGCISRFAVNTAMLTPSVGRLLDPEALPTLKALAMIGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMKPTDEPVNLGYAVAGVRLWVTEAENPNRLAPLGAVGELIVEGRLVTRGYLDDPARTRESFLPNLPWLPSQHALYRTGDLVRYAEDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQVVVEMVIPEGKIRAQASLVAFVSGLTAADVESSSARNFDQSMPMSQIALPGSTIQALEEALPRYMIPSVYFALDTIPLSVNGKADRRRLREIGAALLVSSTAHKNTVDGKSEPVKWTASSKLELTLLELWTTTLGLEAETIYGDDSFFELGGDSVSAMKLVATARDRFKLSLSVPQMFRHPTIHQLAAILGEATGQPESSASSTTEEGFTFSTPDDSSTNDGVDDDFLRLATAQLAQLAQEKGKKVDIAALLKQLQGGSSSCKTPSVSSSSSSSSSRKKKSAKVVSPVEAPAPVPVPFSLLDGGADVVEKIRAQAVEQCKILPGDIEDIYPATALQEGMMALMARTPGVYTTTLTCELPERVNLARLHSAWDKAAEAHPILRTRIILTENNTAVQVVQRAKELPWDAYSLQDGDPLPDLTSNMTLGSTLLRLAEIHRQNQPRMLLVAIHHALYDGWSMPLLKQAVEDVYHGQELWPQPFTPFINYLNEGKPAAQGYWTAHLDGFAGSVFPNLPSINHHIQPTERRTRSLAVPTAPPGSQYTMATKIQAAWAVTVSRYAEAEDIVFGTVSTGRSAPVPSIDRMVGPTITTVPVRISLGNQAERLTSLLQRVQEDGWNRMDHEHLGLQHIRRLGESAAAACNLQTLLVIQPREEPRAKSISTLLSGLQDVAELKGVDTYPLMLVCEPDGVSLHLTAVFDPAVLDAVMLDRMLAHWELVLNQIWSEPDMAVMGLDGVSYRDKQTLVRWNAGEKIADGCAHDAVYEWSVRTPHAPAVFAWDGKWTYEELEKCSSLIASQVLVHGVSSGDFVALYHEKSRWAAAAILAVFKAGGILVTLDPAHPKDRIKDILDQARPRLVLTSQSLLDEARELETPVMVVQFAASQPMPGECFPLPTVSPTQAAYAPFTSGSTGRPKGIPLEHRGLAASTASVARACLLRPASRVLHFASFAFDASMMEHLIAWHAGSCLCIPVETVRQTDLARCIRDFEVTWAFLTPSCLRLISPDDVQSLEALGLGGESMTPEDIFIWGPRLRQIVQLYGPAECSIVAALTEVTKPSENRLIGRPNACRCWVVDPHSPDRLAPLGAVGELVIEGITVGRGYIDDPERTTQAFIPPPTWIQTLYPNEQQPSRLYRTGDLVRYAGTDGKLTFIGRRDGQLKLHGQRIELADVEAHLRPLIPGTQKVVVEMVHSVGNHHPLLAAFVEEILTSQDQVEQVVNLLHPSQTQCALNVKAIDSALSQTVPQYMIPSMYLHISRLPLSASGKLNRRHLRRLVAEFPRQRLSEYAAGSGLAVPNRPATAQEREMQAIWARVLSVDPDTIGVNEDFFRIGGDSISGMQVATRCNAAGMHITSADLFQHRTIEQLMRHLSANGKTGSASISLPPEPVDEWVPLAPIQQLFFEIAPQGPDHFNQSLLLRTSRRVSAEKLAGGLDILVGRHSMLRARFCRDDSGQWSQQVRSRGPYPASAFYRLTTHNHIAPELLSSLLAASQMALSIQEGPLLAVDLVNLTDDTQLVYLVAHHLIIDLVSWRILHAELEEYLQTGSFASTTGSVPFLTWSRAQAEYSANHLTPTLPLPGFQEANDGFDASRYWGISCESNTFGQTSTSTFTLDQTVTDQLFGPANNVLDTRPAEILQAALWYSFTQSLTDRPGPSIYVEGHGREPWTGSIDLSGTVGWFTTMSPLVSAPWDSLSQTSMRDFLDALSYIKDQRRRIPANGWAYFTSRYLNDEGKVAYGRMKPVVEILFNYMGQYQEMNREDAILQLAGDGIQSGTGAADVADNVPRFSLIDVSAFISNGCLTFQFILPKSLQQDSRLQGCFQEYERTLVAAANSLSTEGPRKTLADFPLMPALTYDQLSQCLDHTLPSMGLCARDVVDIYPCSPVQQGMLLAQLRDRQAYQQRFRFQVKSRGSTDRLTLEKLKDAWTEVINRHDILRTLLLPVSDYSHLDQVVMAPGSLQHLVRINAMDTNPTQGLPHSINITSDSTGTVICEWNVSHALVDAMSIAVIQQEVNEALEGSLGQHQKTPRYADYIQWLSLQDNTETQAYWKKYLEGVEPCLFPKLASSTDKVNPEGTISAIRATWTRDSRLDKLCHTHGITLTNLFHIVWSLLLSAYLGTDKVCFGYTTLGRDVPVDGVEKMVGPLVNVIATTIQLQEDDSILDALLTHQTHLSNSLQHQHYALADVYASLGLVGSQLFNTIVSLQDISHFDANDERPTRVEMLPANDVSEYDVALNIGVDQSSIQVVCSYRTLSLSVEQADALLRTTSHVLDEILRDPKQPLRDLEVISPQCKEQLVKWNAAMPAPTDEYIHEKIQDQCRLHSSREAACAWDGIFTFAEVDDLSSRLAARLIRMGVTSGHIIPIYSPKSRWTVIAILGVLKTGAAFTLLETSHPTARLRVICNEIKADIIIAPASHAVPAATLAPILVVLDSITSMSPQESDLLPAVGMPPAAEALAYLIFTSGSTGNPKGVMVTHQNLCSNASIMTTSVNMTSDSRVLHFASHAFDACLWEIFGALFAGACLIIPSESETKEDLAGCIERMVVTWAFLTPSVARILKPEALPSLRNLVLGGEPIAASDLDMWRGHVQVVCAYGPTETAILASTTSPSTFPSDGKDIGVPTGSSLWIVDKQNYNKLAPHGATGELLIEGPNVSQGYLGDPEKTNEAFPVAPRWLSQLRKSPTRVYRTGDLVRFNTSTGTIHFVGRKDNQIKFHGQRIELGDIEHHAQQAFSNASMVIVDLITPEQPQQPYIVAFVHQADTRTGTADPIDTILLPPSESFRADAIGAQNHMHKRLPHYMVPTAFLPLHRLPLSGTGKADRKRLRQCALSLSSLELNAYRATASAKRMPFTAAECKMQELVATVLGRDMSEIGMDDSFFYLGGDSIQAMRLVSEGRQQGLTLSLQAIFDAPRLGDLAYRTANLVRVSEPAPPTLPATSSDDCDHKETIVAALPIKKTDVAEVLPTTSFQRTWLDSQLKSYIVVDIPGPIDLARLRTAIQRVVKAHPILRASFVPYETTTMQVILRTAVVITEADLSTTTVEGICRKDANAPMAPGTPYLRVILATQGEVDRKLIMRLSHAQYDGISLSLLMNDLSHAYASESRPLPSSHLPAFNDYITYQQTQGADPTATTFWHRLLKDVPITYLDLQPAETPTSNGSLITRTRDINIAAFPSLPNGITTATAVKAAWSLVLAQKTGSLAVIFGQVVHGRGIALTGVEGIVGPCANITPVVARLGPQTTRMELMQTLQDQHRSAMPYETAGRKELQTIVQHQNNVMADDMELSLGEARWGKTGYVGGEDHVVDVGVE