Protein
Modification methylase MjaII
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Function
This methylase recognizes the double-stranded sequence GGNC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the MjaII endonuclease.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.
Sequence
MNLDAWLDIQPAKKLYTIKEASRILTKKFGKEIKEHNISYLVQYGRVNKYKIKNRVYVDIDEVENYYKKLFFEKRKEWEEKLGFKLDWDLAFDLLSEKERTKHVHGIHPYKGKFIPQLVEYFLKRHFNVGDIIIDPFMGSGTTLVQCMEMGINSIGIDISPFNCLIAEVKLQKYDIQKLKKILLDMLNKTKEFSKNLGDDEFVKEMDKLIEKYNKKYFTLEYKRKLSKKEIDEDSYSEKIMEMFYLEYKKLKEKYCKNDDEFDDIFKDKPFLYKWYSPRIRAELNFYLNLIKDCRDETIKKVAMIILSRTARSVRGTTHFDLATLKEPVFDPYYCYKHKKICRPVQTILRHLEEYTNDVISRIEEFSKIRKDAYYLIINGDSRTVDIEEELKKHPNFYELYKNKKIDGIFTSPPYLGQIDYHEQHAYAYELFDIPRLDELEIGPKFKGSSKKAQKEYIEGISDVLINMKRFLNEDAKIFIVVNDKKNLYKEIFEKSGLILVREFKRPVLNRTERDRNPYYESIFELKMEE