Protein
DNA replication licensing factor mcm4-A
Function
Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Similarity
Belongs to the MCM family.
Sequence
MSSPTSTPSRRKSKRGRGSNPPTPRGEEVQSPPSQKRRTEDSTSIGELLPMPTSPSGDIQSPLFSSPAPSRHSAHQSELDLSSPLTYGTPSSRVEGTPRSGIRGTPARQRADLGSARKVKQVDLHSDQPAAEELVTSEQSLGQKLVIWGTDVNVAICKEKFQRFVQRFIDPLAKEEENVGLDLNEPIYMQRLEEINVVGEPFLNIDCDHLRSFDQDLYRQLVCYPQEVIPTFDMAANEIFFERYPDSILEHQIQVRPYNALKTRNMRSLNPEDIDQLITISGMVIRTSQIIPEMQESFFKCQVCAFTTRVEIDRGRIAEPSVCKHCNTTHSMALIHNRSMFSDKQMIKLQESPEDMPAGQTPHTTILYAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVRNVKSVYKTHIDVIHYRKTDSKRLHGIDEDTEQKMFTEERVAVLKELAAKPDIYERLAAALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEVNILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPVESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDETYDRRLAHHLVALYYQSEEQLKEEHLDMAVLKDYIAYARTYVNPRLGEEASQALIEAYVDMRKIGSGRGMVSAYPRQLESLIRLSEAHAKVRFSSKVETIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATARKRKEELAQVLKKLIQSKGKTPAFKYQQLFEDLRGQSDAAITKDMFDEALHALADEDYLTVTGKTVRLL