lhpI

Delta(1)-pyrroline-2-carboxylate reductase

316 amino acids

Catalyzes the reduction of Delta(1)-pyrroline-2-carboxylate (Pyr2C) to L-proline, using preferentially NADPH over NADH as the electron donor. Together with LhpH, is involved in a metabolic pathway that converts trans-3-hydroxy-L-proline (t3LHyp) to L-proline. To a much lesser extent, can also reduce Delta(1)-piperideine-2-carboxylate (Pip2C) to L-pipecolate in vitro; however, this activity has likely no physiological significance in vivo since C.psychrerythraea probably possesses no ability to metabolize D-lysine via the L-pipecolate pathway. Does not show ornithine cyclodeaminase (OCD) activity.

Belongs to the ornithine cyclodeaminase/mu-crystallin family.

34.701 kDa

MKIISAEQVHQNLNFEELIPLLKQSFSRPFSMPQRQVYSLAPEQSENHDAFALLPSWNEEVIGNKAFTYFPDNAKKHDLPGLFSKIMLFKRQTGEPLALVDGTSVTYWRTAAISALASQLLSRKNSQHLMLFGTGNLASYLVKAHLTVRDIKQVTLWGRNAKKVSKLIADFSILYPAVTFKTSVDVNAEVASADIICCATGAKTPLFDGNSVSAGCHIDCLGNHMTDARECDTTTILRARVFVDSLTNTLNEAGELLIPMAEDAFNKDEIVGELADMCKTPSMLRQSSDEITLFKSVGTAISDLVAAHSVVEKLAD

lhpI

Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductase

311 amino acids

Catalyzes the reduction of both Delta(1)-pyrroline-2-carboxylate (Pyr2C) and Delta(1)-piperideine-2-carboxylate (Pip2C) to L-proline and L-pipecolate, respectively, using NADPH or NADH as the electron donor. Can also catalyze the reverse oxidation reactions, albeit at a much lower rate. Together with LhpH, is involved in a trans-3-hydroxy-L-proline (t3LHyp) degradation pathway to L-proline, which allows A.brasilense to grow on t3LHyp as a sole carbon source. Also appears to be involved in D-proline and D-lysine metabolism. Does not show ornithine cyclodeaminase (OCD) activity.

Belongs to the ornithine cyclodeaminase/mu-crystallin family.

31.831 kDa

MTALSPIPVFDAADTAALLAYPALLATLGQAVADYAAGEIVSPERLVVPLQAGGVMLSMPSSARDLATHKLVNVCPGNGARGLPTILGQVTAYDASTGEMRFALDGPTVTGRRTAAVTALGIQALHGAAPRDILLIGTGKQAANHAEALAAIFPEARLHVRGTSADSAAAFCAAHRAQAPRLVPLDGDAIPDAIDVVVTLTTSRTPVYREAAREGRLVVGVGAFTADAAEIDANTVRASRLVVDDPAGARHEAGDLIVAQVDWQHVASLADVLGGTFDRSGPLLFKSVGCAAWDLAACRTARDALAARRAG