Function
Activates the 7th to 12th amino acids (Val, D-Val, Trp, D-Leu, Xaa and D-Leu) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 8th (D-Val), the 10th (D-Leu) and 12th (D-Leu) amino acids. The 11th (Xaa) amino acid is Trp in linear gramicidin A; Phe in linear gramicidin B and Tyr in linear gramicidin C.
Sequence
MNSMGDLTDLYTLTPLQEGMLFHSLYSEGSAYMIQTTAILTGELDIVPFEKAWKKVIQRHSILRTGFIWEETEKPLQAVFESVPFSIRQKDWSSYGSDEQESMLAAFLQNEKAAGFDLSEAPLMRVTIIKLGEAVHRLIWSFHHLLLDGWSSPIVFQEVLDFYEAYRQGKDLRLPQARPFKDYVSWLRRQDKTASEQFWREFLGPMENPTPIPFETHAKRTAGHEGLEKQIGEATRALTGQVTKALAKLARTNKVTVNTIVQGAWAILLSRLSGEENVVYGVTGSGRPSDLPGVEQMVGMFINTLPMKAKIEPEQSLADWFKALQEQQSKVRQYEYTSLVDIQGWTDVPRGTPLFESIFVFENYPLGEEDEKEVGFTISAVQHFQEVDNPLTVVGIPGDPFSIKMMYATDRFEQAAIERTLDQLALILEAIVDNPERSLSALSLLRPEERQHLLVGLNDTATNYPSDKTVHQLFAETAARHPERIAAVAGDQQLTYAELEARANQLANYLQKQGVEAGTLVGLCVDRSLDMLIGLLAILKAGGAYVPIDPAYPEERLAFMLADAKISILLTQKHLGKQWKGRKRRTVYLDRDAKKWAEESPLAPDVDTTKDSLAYVIYTSGSTGTPKGVLAVHRGVVRLGQKTRTTSPISEADVFLQASTVSFDAATFEIWGALLNGAKLVLMPPDLPSLDELGEAIVQHKVTTLWLTAGLFSIMVDHNADYLRGVRQLLVGGDVVSVPHVRKVLALGGVTVINGYGPTENTTFTCCYPVTELSEDITSFPIGRPISNTTVYVLDKHKQPVPYGAAGELYIGGDGLALGYLNNAELTAERFVENPFDPQKGSRLYRTGDLVRYLPNGTIEFIGRIDNQVKIRGFRIELGEVEAALALHPEVSETVVMARENDRGEKHLTAYVTVAKDDAPEVADLQAWLKTKLPEYMVPSAYVFLDAMPLTANGKIDRRRLPEPEWGNRSETKAYTEPRNQAEELIASIWSQVLGVEKVGIHDNFFELGGHSLLATRVISRLREVFGVEQSVRSIFEHPTIDAWSEQTAALQLGGPGTDDSSTQIQPVPRDGALPLSFAQQRLWFFDQLMPDNPMYNIPFALRLQGELDVAAWEKSLQAIIARHESLRTTFTDIDGQAVQVIHPQLDWKLDTVDLRDRSSEEKQQASARLAADDAARPFDLRQGPLMRATMIRTEEQAHVFLINMHHIVSDGWSVGVFLRELFAHYEAYSKGEVPQLAPMPIQYADFAAWQREWLEGEVLEQQVAYWKEKLGGAEPLLALPTDRPRPALQSYEGATYTTSFSHDLLAKLKKLSKEAQHDLVHDIARRFPNVLYRYSGQEDIVVGSPVAGRNRQETEKLIGFFVNTLALRTSLSGDLPFTELLARVRETALAAYAHQDVPFEKLVDELQLERSLSYSQLFQVMFVLQNFPLEDVETAGLHVAPVDTESHLTTSKFDLTLTMREKEDTLVAAFEYSTDLFDRTTIERMAEHLQHLLASIVTQPEASLDQLALLGESEWNRLVVEQNETATDYPRDKTAHQLFAETAARYPERIAAVAGDQQLTYAELDTKANQLANYLQKQGVEAGTLVGLCVDRSLDMLVGLLAILKAGGAYVPLDPAYPEERLAFMLADANVSILLTQKHLGKQWKGRKRRTVYLDRDAKKWTAESPLAPAVDATKDSLAYVIYTSGSTGTPKGVLVAHRGIVRLVKNTNYVTITEEDVFLQASTVSFDAATFEIWGALLNGAKLVLMPPDLPSLDELGEAIVQHKVTTLWLTAGLFSIMVDHNADYLRGVRQLLVGGDVVSVPHVRKVLALDGVTVINGYGPTENTTFTCCYPVTELAEEITSFPIGRPISNTTVYVLDKNRQPVPLGVAGELYIGGDGLASGYLNNPELTAERFVDNPFDPQKASRLYRTGDLVRYLPDGAIEFIGRIDNQVKIRGFRIELGEIETALLRHPAVQEAFLMVREDAPGDKRLAAYLVFAGGQTVEPVEMRSYLKDKLPEYMIPSAFVQMDSFPLTPNGKVDRRALPTPEYARSEAASGYVAPATELEVKLADIWKTVLGVADVGIHDNFFELGGDSILSIQIVARANQLGIRLTPKQLFENQTIAELLRVVADSSQLPHTKWENEQGIVTGNVPLTPIQKWFFAADQPSLHHWNQSLLLTVQQPVDVSVLERAIASLLSHHDALRMSFSFVDGAWTQQMNGLGDHTPFRCVDLSDLSTQEQEQAARLEEIASEVQASLNIAEGNVVQAVYFNLGEQKAGRLLLVVHHLVVDGVSWRILLEDLQHAYEQLANHADVSFPAKTTSFKMWAEKLADYADSDALEQEKAYWLQQSSGGSPLPVDHPYEPNENTEAAAKQVTLSLRADETRALLHETLTAYRLQINDVLLAALAKAMQRWTGQKTLHVHLEGHGREEIIEGADLSRTVGWFTSMYPVQLDFDQSKPWGHVLKAVKEQLRHIPQKGIGYGILQYLSNDDEWKEQLQAYTKPEISFNYLGQFDQVVSAGAKFAMAEESRGANIAADAIRAHLIDVNSAISGEQLHITWMYNANIHNQETIEALARDYMESLREIMEHCRSEEAGGYTPSDFPLARLDQRAIDNYVGRDRSIENVYPLTPLQEGMLFHSLYEHAGGDYVVQFSMTMHHVEVDVFQQAWQKVVDRHSILRTSFIWDGVSTPHQIVRKHVQVVVDEQDWRHVPADQQKAEWDAFLEEDRKRSFAITEPPLMRWTLLRISDTAYRFIWSFHHVLLDGWSVPLVMKDWFAAYMALADGKDIQFGAVHPFSQYVAWIQRQDLQAAERFWRNHLKGIYAPTQVNFGQTVQPVGETKSYDERSIRFSAERTRELQAFARQHQVTLNTLVQSAWAMILGTYSKEADVVFGATGSGRPADLPGVENMVGLFINTLPIRVTLDPGKKVREWLRELQELQVELRQYEYTPLVDIHGWSEMARNAPLFESIFIFENYPIDESVKEVDHSFQIADVDSVEQTNYPLTVVCGPGAEFLVKIKFDQSRFDGGRIERVLEQMTLLLQSMTANPDQLLADVNMISQSEQKQVLIEWNETKVDYPTGLCVQQAFEQQVEKTPDAVALIYKDVELTYADLNQRANQLAHRLLAQEVKPDTLVGICVERSPEMIIGIFGVMKAGAAYVPIDPALPQERIAYMVEDSQASILLTQQSLAELLPKTQARVICLDGDSLANEPVANPASEVTEQNLAYVMYTSGSTGLPKGVMVEHHSVVNLAHALIEAFRIQPSSRVLQFTSFSFDVSVSEIVMALLAGAALVIEDREVLLPGPELISVLQQKRITTVSMVSSVLAALPAADLPDLQTLIVGGEAPSRELVARYADRRQFFNCYGPTEATVCSTMMLCNAGMKSAPIGRPLANATLYVLDANQKPVPVGVPGELYIGGKGLARGYWNRPELTAERFIAHPFGAKGERLYRTGDLVRYLPDGNLEFLGRIDTQVKIRGYRIELGEIESALSQHPAIQEAVVIAWEQRLAAYMVAAGEAQPAAEELARYLKETLPDYMIPAGFVFMDAIPLTVNGKVDRRALPAPDWGILATRQEYVAPRTPTEEMVANIWAQLLSVEKIGVHDDFFERGGHSLLATQAISRLRQAFGVELPLRMLFDHPTTAAISTQIASLLQGETALRSQPIVPVPRDQHVPLSFAQQRLWFLDRLIPNSFLYNIPSAARLHGELDVEAWERSLKLLIQRHESLRTTFSDRDGEAVQIIHPAIEWSLGRVDLREWAEAEREAKALQLAIEDAKRPFDLERGPLLRASLLVMAQQEYVFLLNLHHIVADGWSMNVFMEELVTIYEALSAGETPQLAELPLQYADYAAWQRDWLQGDVLEQQLAYWKAKVGGAEPLLALPTDRPRPAVQSHKGAMHTITLPAERLAALKTLSREEGSTLFMTLLAAFQTLLYRYSGQSDIVVGSPVAGRNRQETESLIGFFINTLAMRTDLSGEPTFRDLLGKVRETALEAYAHQDLPFEKLVDELELERSLSYSPLFQVMFVLQNIPMDAQALSHIRLEPFHIGQEGVSAKFDITLTTVELPAGLMATFEYNTDLFDPATIERMAGHYANLLAAVSVNPLQPITAIPLVSDQERKQVLFQWNDTSVPSERDTCVHEQVARIAQQLPNQLAVVSDEGQITYAELDAKANQVANYLHKQGIISETLVGVCLDRSIDMLVAQLGILKAGGAYVPMDPAYPQERLAFMMQDAEMPVVLTQEHLLAQLPEARATFLCLDRDWSLIAEESDVAPVIATNRDNLAYVIYTSGSTGTPKGVEIEHAALLNLVSWHQRAYEVGAEDRATQIAGTAFDASVWEIWPYLTKGATLYLPSEEIRLVPEQLRDWLVASGITISFLPTPLAERLLTLEWPSDAKLRYMLTGGDKLHDYPPATLPFVLANQYGPTENAVVATAGIVPAAAGQVSAPSIGRPIDNVQVYVLDEKLQPVPIGVAGELYIAGDSLARGYLHRPDLTRERFIANPYGQKAGARMYKTGDLVRYLPDGNIEFIGRADDQVSIRGFRVELGEIETALYSHPAVKETIVLVREDMPGMKRLVAYIVQREGQEGQAVQAGDFRSYLKELLPEYMVPAAFVFMADLPLTPNGKVDRRALPAPDLFNSEADGTYVAPATELEIKLAHIWKNVLGLADVGIHDNFFELGGDSILSIQIVSRANQAGIRLTPKQLLANQTIAELASVATVTDESEATKLPNEQGIVTGDVPLTPIQTWFFASEQPSVHHWNQSLLLTVQQPVDLAVLERTIECLLAHHDALRMRYSRTEQGWTQRIEGLPETIPFRSVDLSAFPTTAEQEMRLEEIASEVQASLDLTEGPVVQAVYFQLGAEQPGRLLIVAHHLVVDGVSWRILLEDLQTAYEQLAKGQAVQLAAKTTSFKTWAEQLRLYATSEALRQEKAFWLEQMDEVKPLPIDRIFEPSENTEATVKQVMLSLNAEETRALLQDTLSPYRLQINDVLLAALTKALHRWTGEQTVAIHLEGHGREELIEGADLSRTVGWFTSLYPVQLSVDPTKPWGDTLKAVKEQLRSIPNKGVGYGILRYLSEDAELQKRLAEKAQAEISFNYLGQFDQAVVPESKFGMAQEARGANVGQQAIRQHLLDVNSVIAGEQLHVTWMYSENIHEEATIQQLAHNYLEALREIIAHGQSEAAGGYTPSDFPLARMDQRALDKYLGQNRSIENVYPLSPLQGGMLFHSLYEQEGGDYVVQLAMTVEGLDVEAFEQAWQKVVDRHSILRTSFIWEGLTEPHQVVRKQVKACVEKIDLRHLTPDQQKAELSEYLAADRRRSFEIAVAPLMRWTLFRLSESAYRFTWSFHHVLLDGWSIPIVLKDWFSAYLSLAEGKEVAHSFVQPFAHYVEWVQRQDLQAAEQFWREQLAGFYEPTPLAMGNSTGGRADLPKGYEEQEIRLAKDATARLQAFVRTHQLTLNTLVQGAWALMLGRYGGTDDVVFGATGSGRPADLPGVESMVGLFINTLPIRVALDANQSVREWLRGMQEQQVELRQYEYTPLVDIQGWSEMTRNTALFESIFIFENYPIGESVKDEQHQLRLSDVETIEQTNYPLTVVCGPGEELIVKIKYEQNRFAPEQIERVLQQMSQLLQDMTAKPEQRLQDVSMISERERQQVLVDWNETSVAYPQQLCVHQAFEQQVEKTPDAVALVYKDVELTYAELNERANQLAHRLLAEGVKPDELVGICVERSPEMIVAFLGVMKAGAAYVPLDPAHPQERIAYMIEDSQASVLLTQASLTDRLPASSRQVICLDSDELANEPVTNAETSVGEHNLAYVIYTSGSTGLPKGVMIEHRSVINLAYDLIRHFQIDATSRVLQFISFSFDVSVSEIVMSLLAGATLVIEDRESLLPGPELIRVLQEQRITTFAMVSSVLAALPEADLPDLRTIIVGGEAPSRELVARYATGRQFINCYGPTETTVTATLKHCQDDGKNPPIGRPIANTTVYVLDAHLQPVPIGVPGELYIGGKGVARGYWNRPELTAERFIADPFGQADERLYRTGDLVRYLENGELEFLGRIDDQVKIRGYRIELGEIENALRQHPAVQNVVVIARQEGAGDKRLAAYLVAATGQQPDEAELVRYLKSTLPEYMVPAGYVWLEKIPLTVNGKVDRRALPAPDYGHAETGKAYVAPRKPIEEIVANIWAQVLSVERVGVYDDFFELGGHSLLATQAVSRLKEAFGVNVPLRTLFEHPDVAGMSEKLAGLLEEQSGVTSIPLVPVPRDKQLPLSFAQQRLWFLDRLMPDSALYNIPSAVRIQGQLNIRAWERSLQTIIERHESLRTTFTDIGGEAVQVIHEMMEWRLVEIDLRDLPDEEREAAVQRLEKAEASQPFNLRTGPLLRATLIQTGEEDFVFLLNMHHIVSDGWSMSIFMGELATIYEALSKGDTPQLAEMPLQYADFAAWQRDWLQGEVLEQQLAYWREKLGAAEPMLALPTDRPRPAVQTHHGALYTTAFPLALTEKLHALSRQEGATLFMTLLAAFQTLLYRYSGQDDIIVGSPVAGRNKQETESMIGFFINTLAMRTDMSGAPTFRELLARVRDTALEAYTHQDLPFEKLIDELELERSLSYSPLFQVMFALQNFQMLTREFEGIEIVPFESKNEAVMSKYDISLTMAETQNGLVATFDYNTDLFDHSTIVRMVNHFHQLLEGIVARPDSSIQALPLLATDEREQLVSAWNNTAAAYTYEQTVHELVAAMAEKMPEQLAVVSAEGSLTYAQLDAKANQLANYLQQQGITPETLVGICVERSSEMIVGQLGILKAGGAFVPMDPAYPQERLAFMMADTGMPFVLTQERLLETLPAGDAAFICLDADWEVIAEESTQAPELAVTTDQLAYVIYTSGSTGTPKGVEIEHRALLNLIYWHQHAYTITPDDRASQIAGTAFDAAVWEIWPYMTAGATLYLPQEEIRLIPEKLRDWLVAEGITISFLPTPLAESMLSVDWPSHAALRYVLTGGDKLHHYPAEHVPFTLVNQYGPTENAVVATAGIVAVQAGQVTPPSIGRPIDNVQVYILDEQRQPVPIGVTGELYIAGSSLARGYYKRPDLTQERFVDNPFTANRGAKMYRTGDLVRYLPDGQIEFIGRSDDQVSIRGFRVELGEIESVLYQHPAVKEAIVLAREDMPGVKRLAAYVVVAEDDAEQADDLRGYLKEKLPEYMVPAAFVTLKALPLTPNGKVDRRALPVPEYTAADGEYVAPETFVEKVLAQIWKEVLGVEEVGIHDNFFELGGDSILSIQIVARAGQSGIRLSPKLLFENQTIAELSHAVGDSVHICAEQGLVTGEVPLLPIQHWFFEQKLADRNHWNQSVLLTVQPIDPEILKQAFYHLLGHHDALRLRFYHQNGAWKQAGAEWTDIIPFYVVELGDLPANQQVEQIEKLSGEAQASLHLADGPLLRAVYFDMGAGQEGRLLIVIHHLAVDGVSWRIITEDLNKACNQLLQGKQVQLPPKTSSYKYWAEKLVEYAASDSLTEEADYWLSRLDTEVAALPKDYEHGQNRELAARTVSVALTQEETKVLLQELPAVYQTQINDVLLTALAEAVFVWTGNQTTLVHLEGHGREDIFDDVDLSRTVGWFTSMYTILLDTRGTSSLTEALQTTKEQLRSIPHKGIGYGILRYLNKETAEKFKSLPKAQISFNYLGQLDQAVRDTDSLFGFASEARGDNFNRNSDRQHLLDFGCSVVGGQLVVSVAFSKEIYRKETIEALADYYITALRQLLAHAKTDKAAAPAQSAASNLSEFGWDDEEIADLLDLIQDK