larB

Pyridinium-3,5-biscarboxylic acid mononucleotide synthase

246 amino acids

Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Carboxylates the pyridinium ring of nicotinic acid adenine dinucleotide (NaAD) and cleaves the phosphoanhydride bond to release AMP and generate pyridinium-3,5-biscarboxylic acid mononucleotide (P2CMN) (PubMed:27114550). LarB can hydrolyze NaAD directly or it first forms an adduct with NaAD that releases AMP and reacts with bicarbonate/CO2 to generate P2CMN (PubMed:27114550). Is required for the activation of the lactate racemase LarA (PubMed:24710389). May also be involved in the activation of other nickel-pincer cofactor-dependent enzymes (PubMed:27114550).

25.293 kDa

MATTAEILQQVAAGQLSPTAAAQQLEAGKTAALGFANVDLDRQRRNGFPEVIYGAGKTATQIVGIVQALSQQTLPILTTRLSAEKFAALQPALPTAVYHATAQCMTVGEQPAPKTPGYIAVVTAGTSDQPVAEEAAVTAETFGNRVERVYDVGVAGIHRLFAKLDVIRGARVVIVIAGMEGALASVVGGLVDKPVIAVPTSVGYGTSFQGMTALLTMLNSCASGITVVNIDNGFGAAYSASMVNQM