kshA

Probable 3-ketosteroid-9-alpha-monooxygenase, oxygenase component

399 amino acids

Could catalyze the introduction of a 9alpha-hydroxyl moiety into the ring B of 3-ketosteroid substrates.

44.753 kDa

MGSTDTEDQVRTIDVGTPPERYARGWHCLGLVRDFADGKPHQVDAFGTSLVVFAGEDGKLNVLDAYCRHMGGNLAQGSVKGNTIACPFHDWRWRGDGKCAEIPYARRVPPLARTRTWPVAEVSGQLFVWHDPQGSKPPAELAVPEVPTYGDPGWTDWVWNSIEVTGSHCREIVDNVVDMAHFFYVHYGMPTYFRNVFEGHTATQVMRSLPRADAVGVSQATNYSAESRSDATYYGPSYMIDKLWSAGRDPESTPNIYLINCHYPISPTSFRLQYGVMVERPEGVPPEQAEQIAQAVAQGVAIGFEQDVEIWKNKSRIDNPLLCEEDGPVYQLRRWYEQFYVDVEDIRPEMVNRFEYEIDTTRALTSWQAEVDENVAAGRSAFAPNLTRAREAASAESGS

kshA

3-ketosteroid-9-alpha-monooxygenase, oxygenase component

394 amino acids

May be involved in the degradation of cholic acid, a steroid acid found predominantly in the bile (PubMed:21642460). In vitro, catalyzes the introduction of a 9alpha-hydroxyl moiety into the ring B of 3-ketosteroid substrates such as 1,4-androstadiene-3,17-dione (ADD), 4-androstene-3,17-dione (AD), 4-androstene-17beta-ol-3-one (testosterone), 4-pregnene-3,20-dione (progesterone), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 23,24-bisnorcholesta-4-ene-22-oate, 3-oxo-23,24-bisnorcholaesta-1,4-dien-22-oate (1,4-BNC), 23,24-bisnorcholesta-1,4-diene-22-oate and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester (1,4-BNC-CoA) (PubMed:21642460, PubMed:25049233). KshA1 has the highest specificity for steroids possessing an isopropionyl side chain at C17 (PubMed:25049233).

45.02 kDa

MSLGTSEQSEIREIVAGSAPARFARGWHCLGLAKDFKDGKPHSVHAFGTKLVVWADSNDEIRILDAYCRHMGGDLSQGTVKGDEIACPFHDWRWGGNGRCKNIPYARRVPPIAKTRAWHTLDQDGLLFVWHDPQGNPPPADVTIPRIAGATSDEWTDWVWYTTEVDTNCREIIDNIVDMAHFFYVHYSFPVYFKNVFEGHVASQFMRGQAREDTRPHANGQPKMIGSRSDASYFGPSFMIDDLVYEYEGYDVESVLINCHYPVSQDKFVLMYGMIVKKSDRLEGEKALQTAQQFGNFIAKGFEQDIEIWRNKTRIDNPLLCEEDGPVYQLRRWYEQFYVDVEDVAPEMTDRFEFEMDTTRPVAAWMKEVEANIARKAALDTETRSAPEQSTTAG

kshA

3-ketosteroid-9-alpha-monooxygenase, oxygenase component

390 amino acids

Probably involved in the degradation of cholesterol (PubMed:21642460). In vitro, catalyzes the introduction of a 9alpha-hydroxyl moiety into the ring B of 3-ketosteroid substrates such as 1,4-androstadiene-3,17-dione (ADD), 4-androstene-3,17-dione (AD), 4-androstene-17beta-ol-3-one (testosterone), 4-pregnene-3,20-dione (progesterone), 19-nor-4-androstene-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, 5alpha-androstane-17beta-ol-3-one (stanolon), 11beta-hydrocortisone, 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 23,24-bisnorcholesta-4-ene-22-oate, 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4-BNC) and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester (1,4-BNC-CoA) (PubMed:21642460, PubMed:25049233). KshA5 has the broadest substrate range without a clear substrate preference and is active with Delta-4, Delta-1,4, 5alpha-H and 5beta-H steroids, as well as with steroids having bulky aliphatic side chains and an isopropionyl side chain at C17.

44.249 kDa

MSIDTARSGSDDDVEIREIQAAAAPTRFARGWHCLGLLRDFQDGKPHSIEAFGTKLVVFADSKGQLNVLDAYCRHMGGDLSRGEVKGDSIACPFHDWRWNGKGKCTDIPYARRVPPIAKTRAWTTLERNGQLYVWNDPQGNPPPEDVTIPEIAGYGTDEWTDWSWKSLRIKGSHCREIVDNVVDMAHFFYIHYSFPRYFKNVFEGHTATQYMHSTGREDVISGTNYDDPNAELRSEATYFGPSYMIDWLESDANGQTIETILINCHYPVSNNEFVLQYGAIVKKLPGVSDEIAAGMAEQFAEGVQLGFEQDVEIWKNKAPIDNPLLSEEDGPVYQLRRWYQQFYVDVEDITEDMTKRFEFEIDTTRAVASWQKEVAENLAKQAEGSTATP

kshA

3-ketosteroid-9-alpha-monooxygenase, oxygenase component

386 amino acids

Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. KSH is also able to use 4-androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4-BNC), 3-oxo-23,24-bisnorcholesta-4-en-22-oyl-coenzyme A thioester (4-BNC-CoA) and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester (1,4-BNC-CoA) as substrates.

44.268 kDa

MSTDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDLKVLDGYCRHMGGDLSEGTVKGDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTDVRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWNRILIEGSNCRDIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMINWLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKGFLQDVEIWKHKTRIDNPLLVEEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRANEFWNAEVEKNLKSREVSDDVPAEQH

kshA

3-ketosteroid-9-alpha-monooxygenase, oxygenase component

383 amino acids

Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A.

43.668 kDa

MATETVGIREIDTGALPDRYARGWHCLGPVKNFSDGKPHSVNIFGTKLVVFADSKGELNVLDAYCRHMGGDLSKGTVKGDEVACPFHDWRWGGDGKCKLVPYAKRTPRLARTRSWHTDVRGGLLFVWHDHEGNPPQPEVRIPEIPEWHSGEWTDWKWNSMLIEGSNCREIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVNDLGTAYGEAKLDSEASYFGPSFMINWLHNTYGEFKAESILINCHYPVTQDSFVLQWGVIVEKPKGLDDATTEKLADAFTEGVSKGFLQDVEIWKHKTRIDNPLLVEEDGAVYQMRRWYQQFYVDVADITPDMTDRFEMEVDTTAAVEKWNIEVQENLKAQAEAEKAEQSS

kshA

3-ketosteroid-9-alpha-monooxygenase, oxygenase component

382 amino acids

In vitro, catalyzes the introduction of a 9alpha-hydroxyl moiety into the ring B of 3-ketosteroid substrates such as 1,4-androstadiene-3,17-dione (ADD), 4-androstene-3,17-dione (AD), 4-androstene-17beta-ol-3-one (testosterone), 4-pregnene-3,20-dione (progesterone), 23,24-bisnorcholesta-4-ene-22-oate and 23,24-bisnorcholesta-1,4-diene-22-oate.

43.954 kDa

MAQIREIDVGEVRTRFARGWHCLGLSRTFKDGKPHAVEAFGTKLVVWADSNGEPKVLDAYCRHMGGDLSQGEIKGDSVACPFHDWRWGGNGKCTDIPYARRVPPLARTRSWITMEKHGQLFVWNDPEGNTPPPEVTIPEIEQYGSDEWTDWTWNQIRIEGSNCREIIDNVVDMAHFFYIHYAFPTFFKNVFEGHIAEQYLNTRGRPDKGMATQYGLESTLESYAAYYGPSYMINPLKNNYGGYQTESVLINCHYPITHDSFMLQYGIIVKKPQGMSPEQSDVLAAKLTEGVGEGFLQDVEIWKNKTKIENPLLCEEDGPVYQLRRWYEQFYVDVADVTEKMTGRFEFEVDTAKANEAWEKEVAENLERKKREEEQGKQEAEV

kshA

3-ketosteroid-9-alpha-monooxygenase, oxygenase component

378 amino acids

In vitro, catalyzes the introduction of a 9alpha-hydroxyl moiety into the ring B of 3-ketosteroid substrates such as 1,4-androstadiene-3,17-dione (ADD), 4-androstene-3,17-dione (AD), 4-androstene-17beta-ol-3-one (testosterone), 4-pregnene-3,20-dione (progesterone), 19-nor-4-androstene-3,17-dione (nordion), 1-(5alpha)-androstene-3,17-dione, 5alpha-androstane-3,17-dione and 5beta-androstane-3,17-dione (PubMed:19561185, PubMed:21642460). KSH has the highest activity with 3-keto-delta4 steroid substrates (PubMed:19561185).

43.507 kDa

MTVPQERIEIRNIDPGTNPTRFARGWHCIGLAKDFRDGKPHQVKVFGTDLVVFADTAGKLHVLDAFCRHMGGNLARGEIKGDTIACPFHDWRWNGQGRCEAVPYARRTPKLGRTKAWTTMERNGVLFVWHCPQGSEPTPELAIPEIEGYEDGQWSDWTWTTIHVEGSHCREIVDNVVDMAHFFYVHFQMPEYFKNVFDGHIAGQHMRSYGRDDIKTGVQMDLPEAQTISDAFYYGPSFMLDTIYTVSEGTTIESKLINCHYPVTNNSFVLQFGTIVKKIEGMSEEQAAEMATMFTDGLEEQFAQDIEIWKHKSRIENPLLTEEDGPVYQLRRWYNQFYVDLEDVTPDMTQRFEFEVDTSRALESWHKEVEENLAGTAE