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iscU

Gene
Iscu
Protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
Organism
Mus musculus
Length
168 amino acids
Function
Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity).
Similarity
Belongs to the NifU family.
Mass
18.098 kDa
Sequence
MAAATGAGRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQESKKEEPEKQ

Gene
Iscu
Protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
Organism
Mus musculus
Length
168 amino acids
Function
Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity).
Similarity
Belongs to the NifU family.
Mass
18.098 kDa
Sequence
MAAATGAGRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQESKKEEPEKQ

Gene
ISCU
Protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
Organism
Homo sapiens
Length
167 amino acids
Function
Isoform 2: Functions as a cytoplasmic scaffold protein for the de novo synthesis of iron-sulfur clusters in the cytoplasm.
Similarity
Belongs to the NifU family.
Mass
17.999 kDa
Sequence
MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK

Gene
ISCU
Protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
Organism
Homo sapiens
Length
167 amino acids
Function
Isoform 2: Functions as a cytoplasmic scaffold protein for the de novo synthesis of iron-sulfur clusters in the cytoplasm.
Similarity
Belongs to the NifU family.
Mass
17.999 kDa
Sequence
MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Streptococcus pyogenes serotype M1
Length
159 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
17.142 kDa
Sequence
MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVISLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQENPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Streptococcus pyogenes serotype M1
Length
159 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
17.142 kDa
Sequence
MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVISLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQENPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Aquifex aeolicus (strain VF5)
Length
157 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (Probable).
Similarity
Belongs to the NifU family.
Mass
17.531 kDa
Sequence
MSFEYNEKVLDHFLNPRNVGVLEDANGVGQCGNPACGDAMLFTIKVNPENDVIEDVRFKTFGCGSAIAVSSMLTEMVKGKPIQYALNLTYKDIFEELGGLPPQKIHCTNLGLETLHVAIKDYLMKQGRVEEASKIPDCYEEEEEQEESKEFEFLSGT

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Aquifex aeolicus (strain VF5)
Length
157 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (Probable).
Similarity
Belongs to the NifU family.
Mass
17.531 kDa
Sequence
MSFEYNEKVLDHFLNPRNVGVLEDANGVGQCGNPACGDAMLFTIKVNPENDVIEDVRFKTFGCGSAIAVSSMLTEMVKGKPIQYALNLTYKDIFEELGGLPPQKIHCTNLGLETLHVAIKDYLMKQGRVEEASKIPDCYEEEEEQEESKEFEFLSGT

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Length
142 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
16.071 kDa
Sequence
MVFKMMYSEAILDYANSKKFRGKLDDATVIEEGKNISCGDEITLYLKVEDGVVKDAKFEGMGCVISQASASLMLERIIGERVEEIFSLIEEAEKMSRGENFDEGKLKNVTLMSDIKNYPARVKCFILAWKTLKEALKKISRP

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Length
142 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
16.071 kDa
Sequence
MVFKMMYSEAILDYANSKKFRGKLDDATVIEEGKNISCGDEITLYLKVEDGVVKDAKFEGMGCVISQASASLMLERIIGERVEEIFSLIEEAEKMSRGENFDEGKLKNVTLMSDIKNYPARVKCFILAWKTLKEALKKISRP

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Rickettsia prowazekii (strain Madrid E)
Length
131 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
14.223 kDa
Sequence
MAYSKKVIDHYENPRNVGSLDKKKKNVGTGLVGAPACGDVMKLQIEVGDDEIITDAKFKTFGCGSAIASSSLVTEWIKGKSVEDAKEIKNTEIAKELSLPPVKLHCSLLAEDAIKAAIADYKQKRENKKDS

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Rickettsia prowazekii (strain Madrid E)
Length
131 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
14.223 kDa
Sequence
MAYSKKVIDHYENPRNVGSLDKKKKNVGTGLVGAPACGDVMKLQIEVGDDEIITDAKFKTFGCGSAIASSSLVTEWIKGKSVEDAKEIKNTEIAKELSLPPVKLHCSLLAEDAIKAAIADYKQKRENKKDS

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Azotobacter vinelandii
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Cluster assembly on IscU homodimers proceeds sequentially from 1 2Fe-2S per dimer, to 2 2Fe-2S per dimer and finally 1 4Fe-4S per dimer. Subsequently IscU transfers the Fe-S cluster to other acceptor proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters. The clusters are very sensitive to O(2).
Similarity
Belongs to the NifU family.
Mass
13.875 kDa
Sequence
MAYSDKVIDHYENPRNVGKLDAQDPDVGTGMVGAPACGDVMRLQIKVNEQGIIEDAKFKTYGCGSAIASSSLATEWMKGRTLEEAETIKNTQIAEELALPPVKIHCSVLAEDAIKAAVRDYKHKKGLV

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.728 kDa
Sequence
MAYSKKVMDHYENPRNVGSFSNSDSNVGSGLVGAPACGDVMKLQIKVNEQGIIEDACFKTYGCGSAIASSSLVTEWIKGKSITEAEAIKNTSIVEELELPPVKIHCSILAEDAIKAAISDYKSKKNKN

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Escherichia coli O157:H7
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (Probable).
Similarity
Belongs to the NifU family.
Mass
13.849 kDa
Sequence
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.849 kDa
Sequence
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Escherichia coli (strain K12)
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Exists as 2 interconverting forms, a structured (S) and disordered (D) form. The D-state is the preferred substrate for IscS. Converts to the S-state when an Fe-S cluster is assembled, which helps it dissociate from IscS to transfer the Fe-S to an acceptor. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters.
Similarity
Belongs to the NifU family.
Mass
13.849 kDa
Sequence
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Shigella flexneri
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.849 kDa
Sequence
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Azotobacter vinelandii
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Cluster assembly on IscU homodimers proceeds sequentially from 1 2Fe-2S per dimer, to 2 2Fe-2S per dimer and finally 1 4Fe-4S per dimer. Subsequently IscU transfers the Fe-S cluster to other acceptor proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters. The clusters are very sensitive to O(2).
Similarity
Belongs to the NifU family.
Mass
13.875 kDa
Sequence
MAYSDKVIDHYENPRNVGKLDAQDPDVGTGMVGAPACGDVMRLQIKVNEQGIIEDAKFKTYGCGSAIASSSLATEWMKGRTLEEAETIKNTQIAEELALPPVKIHCSVLAEDAIKAAVRDYKHKKGLV

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.728 kDa
Sequence
MAYSKKVMDHYENPRNVGSFSNSDSNVGSGLVGAPACGDVMKLQIKVNEQGIIEDACFKTYGCGSAIASSSLVTEWIKGKSITEAEAIKNTSIVEELELPPVKIHCSILAEDAIKAAISDYKSKKNKN

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Escherichia coli O157:H7
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (Probable).
Similarity
Belongs to the NifU family.
Mass
13.849 kDa
Sequence
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.849 kDa
Sequence
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Escherichia coli (strain K12)
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Exists as 2 interconverting forms, a structured (S) and disordered (D) form. The D-state is the preferred substrate for IscS. Converts to the S-state when an Fe-S cluster is assembled, which helps it dissociate from IscS to transfer the Fe-S to an acceptor. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters.
Similarity
Belongs to the NifU family.
Mass
13.849 kDa
Sequence
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Shigella flexneri
Length
128 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.849 kDa
Sequence
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)
Length
127 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.711 kDa
Sequence
MAYSKKVMDHYENPRNVGSFSSTDLNVGSGLVGAPACGDVMKLQIKVNEKGIIEDACFKTYGCGSAIASSSLVTEWVKGKSIEEAESIKNTTIVEELDLPPVKIHCSILAEDAIKAAISDYKRKKIN

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)
Length
127 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.711 kDa
Sequence
MAYSKKVMDHYENPRNVGSFSSTDLNVGSGLVGAPACGDVMKLQIKVNEKGIIEDACFKTYGCGSAIASSSLVTEWVKGKSIEEAESIKNTTIVEELDLPPVKIHCSILAEDAIKAAISDYKRKKIN

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Length
126 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.399 kDa
Sequence
MAYSEKVIDHYENPRNVGSLDKKDSNVGTGMVGAPACGDVMQLQIKVDDNGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLEEAGAIKNSQIAEELELPPVKVHCSILAEDAIKAAIADYKAKQG

Gene
iscU
Protein
Iron-sulfur cluster assembly scaffold protein IscU
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Length
126 amino acids
Function
A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Similarity
Belongs to the NifU family.
Mass
13.399 kDa
Sequence
MAYSEKVIDHYENPRNVGSLDKKDSNVGTGMVGAPACGDVMQLQIKVDDNGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLEEAGAIKNSQIAEELELPPVKVHCSILAEDAIKAAIADYKAKQG