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hsdM

Gene
hsdM
Protein
Type I restriction enzyme EcoKI M protein
Organism
Escherichia coli (strain K12)
Length
529 amino acids
Function
The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. The EcoKI enzyme recognizes 5'-AACN(6)GTGC-3'.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family.
Mass
59.307 kDa
Sequence
MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETGQEAEYLPEGYRWDDLKSRIGQEQLQFYRKMLVHLGEDDKKLVQAVFHNVSTTITEPKQITALVSNMDSLDWYNGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELVPGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTNITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHLHTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTPFTDEHLQPFERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQHLATSRWRKFSREWIRTAKSDSLDISWLKDKDSIDADSLPEPDVLAAEAMGELVQALSELDALMRELGASDEADLQRQLLEEAFGGVKE

Gene
hsdM
Protein
Type I restriction enzyme StySPI M protein
Organism
Salmonella potsdam
Length
529 amino acids
Function
Methylation of specific adenine residues; required for both restriction and modification activities.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family.
Mass
58.906 kDa
Sequence
MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETGQEADYLPEGYRWDDLKSRIGQEQLQFYRNLLVHLGADEKKLVQAVFQNVNTTITQPKQLTELVSSMDSLDWYNGDHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDAQDFQIKKAFVGLELVPGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPQADIVATNPPFGSAAGTNITRTFVHPTSNKQLCFMQHIIETLPPGGRAAAVVPDNVLFEGGKGTDIRRDLMDKCHLHTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTPFTEQHLQPFETVYGEDPHGLSPRTEGEWSFNAEESEVADSEENKNADQHQATSRWRKFSREWIRTAKSDSLDISWLKDKDSIDADSLPEPDVLAAEAMGELVQALGELDALMRELGAGDEADAQRQLLEEAFGGVKA

Gene
hsdM
Protein
Type I restriction enzyme StySJI M protein
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Length
529 amino acids
Function
Methylation of specific adenine residues; required for both restriction and modification activities (By similarity). The StySJI enzyme recognizes 5'-GAGN(6)GTRC-3'.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family.
Mass
59.311 kDa
Sequence
MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETGQEADYLPEGYRWDDLKSRIGQDQMQFYRNLLVQLGSDEKKLVQAVFHNVSTTIEQPKQLTELVSYMDALDWYNGNHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELVPGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTNITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHLHTILRLPTGIFYAQGVKTNVLFFTKGTVTNPHQDKNCTDDVWVYDLRTNMPSFGKRTPFTEQHLQPFETVYGEDPHGLSPREEGEWSFNAEESEVADSEENKNTDQHQATSRWRKFSREWIRSAKSDSLDISWLKDKDSIDADSLPEPDVLAAEAMGELVQALGELDALMRELGAGDEADAQRQLLNEAFGEVKA

Gene
hsdM
Protein
Type I restriction enzyme EcoR124II M protein
Organism
Escherichia coli
Length
520 amino acids
Function
Methylation of specific adenine residues; required for both restriction and modification activities (By similarity). The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3'.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family.
Mass
58.013 kDa
Sequence
MKMTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFISENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDRLNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVEGLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHVNKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKADFAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFGTTIAVNILVLSKHKTDTNVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHLAKSVAFETVVANDYNLSVSSYVEAKDNREIIDIAELNAELKTTVSKIDQLRKDIDAIVAEIEGCEVQK

Gene
hsdM
Protein
Type I restriction enzyme EcoprrI M protein
Organism
Escherichia coli
Length
520 amino acids
Function
The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. The EcoprrI enzyme recognizes 5'-CCAN(7)ATGC-3'.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family.
Mass
58.015 kDa
Sequence
MKMTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFISENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDRLNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVEGLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHVNKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKADFAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFGTTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHLAKSVAFETVVANDYNLSVSSYVEAKDTREIIDIAELNAELKTTVSKIDQLRKDIDAIVAEIEGCEVQK

Gene
hsdM
Protein
Type I restriction enzyme EcoEI M protein
Organism
Escherichia coli
Length
490 amino acids
Function
The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. The EcoEI enzyme recognizes 5'-GAGN(7)ATGC-3'.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family.
Mass
55.62 kDa
Sequence
MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDTQEEELELEQDDYQFPIPQRYLWRSWAANSEGITGDALLEFVNDDLFPTLKNLTAPIDKNPRGFVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFSSSQERHLFGDIYEQILRDLQSAGNAGEFYTPRAVTRFMVNRIDPKLGESIMDPACGTGGFLACAFDHVKDNYVKTTEDHKTLQQQIYGVEKKQLPHLLCTTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQVDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIIEVLADKGRAAVVLPDGTLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNILFFTKGQPTKEVWFYEHPYPDGVKNYSKTKPMKFEEFQAEIDWWGNEADDFASREENNQAWKVGIDDIIARNFNLDIKNPYQGETISHDPDELLAQYQTQQAEIGELRNQLRDILGAALAGNKGAN