hpaH

4-hydroxyphenylacetate 3-monooxygenase oxygenase component

494 amino acids

Utilizes FADH(2) supplied by HpaI, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA).

Belongs to the FADH(2)-utilizing monooxygenase family.

56.269 kDa

MKMPAKTGKEYMERLKQAKSSVYIHGEKVEDVTVHPAFRNVVRSMAALYDRQYEKPEKMLYRSPTTGQPVGMTFIQPTTIDELIARREATQEWARMSAGMMGRSPDYLNAEVMAMGIANDLFAEDDPMFAENAKNYYEYAREHDISLTHTLIHPQMNRAKALHEQNDADVPLHLVERRKDGIIVSGIRLLATQGGITDEILVFPSTVKKATSGEDPYALAFAIPNNTPGVKFICREAFDYGRSAWDHPLASRFEEGDAIVSFENVFVPWERVFVCGNSSICNRTFRETNAVVHMSHQVVAKNIVKTEFLLGVTLCLIEAIGIGEFQHVKDKGAEIMLVLETMKSHLYRAEHNAKRDRWGTMTPDFAALDAARNWYPRIYPRLAEIIRILGASGLMAIPTEADFQHEEIGDIVRRAMQGATVDGYERVQLFRLAWDLTMSAFGARQTHYEYYFFGDPVRMGMAYFDGYEKEPYKQFVREFLRGAKSVFIPADNKH

hpaH

Anthranilate 3-monooxygenase oxygenase component

494 amino acids

Utilizes FADH(2) supplied by NAD(P)H-dependent FAD/FMN reductase (GTNG_3158) to catalyze the hydroxylation of anthranilate producing 3-hydroxyanthranilate. Preferred substrate is anthranilate but it can also utilize 2-hydroxyphenylacetate, 4-hydroxyphenylacetate and salicylate but not kynurenine, phenol, 2-NBA, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene.

Belongs to the FADH(2)-utilizing monooxygenase family.

56.164 kDa

MRMGIRTGAQYISGLKSRKPEIWLSGRRVINVCEEPVFKQPIREIARLYDMQHDPEYQDKITHICTETGERVSNAFLVPKSREDLLARRALFEVWARATFGLMGRTPDFLNVVLTSLYSNASFLEKYNPQWAENIRAYYRYVRDNDLFLTHAIINPQNDRSKPSHEQQDTFTHLGVVRETPEGLIVRGAKMLATLAPITDEVIIYTFPGYKPGDERYAVSFAIPIDTPGLRILCREPMQDGTRPLFDHPLASRFEEMDALLVFNDVLVPWDRVFIYNNVEAANLLYPKTGIAQQPAHQTGVRGLIKLQFATEVAIRLADSIGVDVYLNVQNDLGELLQSVEAIRALLHLAEHELEVLPSGEVMPGWVPLETIRGLLPKLYPRAVEVLQIIGAGGLLMSPTGADFANPELAADMEKYYAGRIGVGGEERVRLFKLAWDLCGEAFGQRLLQYERFYTGDPIRKRAIFYNNIKRERTLVMVDEALRMPNQQEKVVNA