hpaB

4-hydroxyphenylacetate 3-monooxygenase oxygenase component

520 amino acids

Utilizes FADH(2) supplied by HpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives.

Belongs to the FADH(2)-utilizing monooxygenase family.

58.848 kDa

MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPAFRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRHERDAIAEWSRLSYGWMGRTPDYKAAFGCALGGTPGFYGQFEQNARNWYTRIQETGLYFNHAIVNPPIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLLYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRGVQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNIIERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHNNDDINMLDKLLK

hpaB

4-hydroxyphenylacetate 3-monooxygenase oxygenase component

520 amino acids

Utilizes FADH(2) supplied by HpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA.

Belongs to the FADH(2)-utilizing monooxygenase family.

58.651 kDa

MKPENFRADTKRPLTGEEYLKSLQDGREIYIYGERVKDVTTHPAFRNAAASVAQLYDALHNPELQNTLCWGTDTGSGGYTHKFFRVAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGGGLGANPGFYGQFEQNARDWYTRIQETGLYFNHAIVNPPIDRHKPADEVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDAEGDKLISRASYELVAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKRSLECTGTLEFRGVQAELGEVVAWRNMFWALSDSMCAEATPWVNGAYLPDHAALQTYRVMAPMPYAKIKNIIERSVTSGLIYLPSSARDLNNPQINDTLAKYVRGSNGMDHVERIKILKLMWDAIGSEFGGCHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHNNTDINMLDKLLK

hpaB

4-hydroxyphenylacetate 3-monooxygenase oxygenase component

520 amino acids

Oxygenase component of the 4-hydroxyphenylacetate (HPA) 3-hydroxylase. Catalyzes the hydroxylation of 4-hydroxyphenylacetate to form 3,4-dihydroxyphenylacetate, using FADH(-) provided by the reductase component HpaC to activate oxygen. To a lesser extent, can also use reduced FMN (PubMed:20000468). In vitro, has hydroxylation activity toward tyrosol and various cinnamic acid derivatives, catalyzing the hydroxylation of p-coumaric acid, caffeic acid, ferulic acid, and coniferaldehyde (PubMed:23666444).

Belongs to the FADH(2)-utilizing monooxygenase family.

58.464 kDa

MKPEDFRASATRPFTGEEYLASLRDDREIYIYGDRVKDVTSHPAFRNAAASMARLYDALHDPQSKEKLCWETDTGNGGYTHKFFRYARSADELRQQRDAIAEWSRLTYGWMGRTPDYKAAFGSALGANPGFYGRFEDNAKTWYKRIQEACLYLNHAIVNPPIDRDKPVDQVKDVFISVDEEVDGGIVVSGAKVVATNSALTHYNFVGQGSAQLLGDNTDFALMFIAPMNTPGMKLICRPSYELVAGIAGSPFDYPLSSRFDENDAILVMDKVFIPWENVLIYRDFERCKQWFPQGGFGRLFPMQGCTRLAVKLDFITGALYKALQCTGSLEFRGVQAQVGEVVAWRNLFWSLTDAMYGNASEWHGGAFLPSAEALQAYRVLAPQAYPEIKKTIEQVVASGLIYLPSGVRDLHNPQLDKYLSTYCRGSGGMGHRERIKILKLLWDAIGSEFGGRHELYEINYAGSQDEIRMQALRQAIGSGAMKGMLGMVEQCMGDYDENGWTVPHLHNPDDINVLDRIRQ