Protein
E3 ubiquitin-protein ligase hecd-1
Organism
Caenorhabditis elegans
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Involved in the ubiquitination and proteasomal-mediated degradation of cytoplasmic and mitochondrial proteins (PubMed:24703696, PubMed:21673654). Positively regulates lin-12 activity in the anchor cell (AC)/vulval precursor (VU) cell fate decision (PubMed:25552605). Negatively regulates glp-1 activity in germline proliferation (PubMed:25552605). May play a role in the formation of fibrous organelles, a hemidesmosome-like structure attaching muscles to the epidermis (PubMed:20153198). Regulates germline DNA double-strand-break repair and apoptosis in response to DNA damage by recruiting E4 ubiquitin-protein ligase ufd-2 to DNA repair foci (PubMed:27669035).
Similarity
Belongs to the UPL family. K-HECT subfamily.
Sequence
MDGIDPETLLEWLQTGIGDERDLQLMALEQLCMLLLMADNIDRCFESCPPRTFIPALCKIFIDETAPDNVLEVTARAITYYLDVSNECTRRITQVDGAVKAICTRLAAADISDRSSKDLAEQCVKLLEHVCQRETMAVYDAGGINAMLTLVRVHGTQVHKDTMHSAMSVVTRLCGKMEPTDPELGKCAESLGALLEHEDPKVSESALRCFAALTDRFVRKMMDPAELAMHSNLVEHLISIMVASNDENSPTTASANILSIVLSLIGNLCRGSSLITEKVLTSPNMITGLKATLTNKEERVVTDGLRFCDLLLVLLCEGRSALPLTSVVSGDYAAGSGAERVHRQLIDAIRQKDLTALVDAIESGQVDVNFADDVGQSLTNWASAFGSIEMVQYLCDKGSDVNKGHKSSSLHYAACFGRPDVVKLLLQRGANPDLRDEDGKTALDKARERSDDDHNQVANILESPSAFMRNKEDPKVKASTSKQPGTSTKPELPNPNLVRKVLHQLLPIFCEIFQKSLNGSVRRTSLSLMRKIVENIGDLRQSAVGDGNAPAAQSARKMSTDVSAGAESLVAVVVSVMDQEDDHEGHEQVLLILESLLEKDAELWVIELVRLGVFERVEAMAKEPPKGLEEVLNAIHLEGRSRVTPMEIDFENQPSSSTAVPTANDIMDTTVPSSSGGADAESNSNPSTIEMADPESSTPSSSTQQSISKPKATASSTASSAILQVVSKLSSVASLDKSAAAVDKKPTKTVLSQGTPYRWKEWRIVRGTTSLFIWSDVLLIELPFQSNGWFRYLADNDSHVQFVTGTASVDQQMTEEEKDNFQKTERREMVSRWNAVKGVFDDDWSSVPIAVLGIPSNAKKVSQKLEVPAWELWSSKSSELQIKSISSSAPTGQANTMLTTIKDDAGGFLFETGTGRKTNVMPEHALPSDFHTGWSSHGVSTRKMKFRQDIQKRKVQELAWKLWNDHLKEAHAKPREALVRLENAARTIESTIRHVKAQSNFKHRNVKQPRIERVQEYCAAISTLHESIVDDRRLSTFEFSVSGIVPALFGLLSMMEKFPDSFPSRIFKEQFSKGEALSYLALKIVAVLEANEKFPQHLYDSPGGSSFGLQLLSRRVRTKLEMLPRADGKENNDENLVNKTGKIVKCEPLASVGAIRAYLHRMVTRQWHDRERANYRYVKEIQELKTKGKSIELRHVSDFDENGVIYWIGTNGRAAPLWTNPATVKAVKITCSDTRQPFGKPEDLLSRDQNPINCHTSDDKNAHFTIDLGLFVVPTSYSLRHSRGYGRSALRNWMLQGSVDAKRWENVIVHTDDKGLGEPGSTATWHVGEKGTTAFRFFRIAQNGKNSSGQTHYLSCSGFEIYGDIVDVVTEAICEDPPKKDSPAGTSSTPGSSSSAALPPLTKEQVLEMLPARENNNRLKSGLSLETVTSMLQRSRHRSRGSYKISESKSKVVRGKDWRWEDQDGGEGKFGRITSPPESGWVDVTWDNGNANSYRFGANGNFDIERVTSTGHRYSTPSLASHVPTSVMEAVRRNRAFYTPKTTGGPPSSSVFGTSSSAGSSRGAASALSRFASVKNTTPAGTPSSGGSSGGAIGKKSMSTTNLVDERQKTSGPSVASTGQAASAESLQHQTPSLENLLARAMPHAFGRIAENQEPEDEPMGGEESDSAASMRSAASSNSQMSMGSSSQQQQQQDSDMTPRDSAGTPSTPRDDKNQTLSVSAPDLAAARQRQASAETDGDADADETNSEDKTVGADDAMEEDDEEEETMEDEEDDDDDDDDESSNENQEKLVELLGGERGLFDKLKEVITGESLSDASSSAKDATTNEAQKKGGKKPKKWFKKMSSYTDVLKGLMQNRYPVALLDPAAAGIEMDEMMDDDDYYDFSEDGPDDGDSVEDEVAAHLGMPVDSFASMVAARTPITWRQFSELMSGSNRERAAMARAVASSRGSPWEDDATVKCTFEALIPAFDPRPGRSNVNQTLEVELPTVVKDFGSTKASSSKIDKDDQMRFFLRGPNMSGVDNVTIEMNDDSASLFRYMQIINNSVNWATKSDRSRRIWEPTYSICYCSADQTNVEVSKIPDEESSTPCQVNQCLETIGLLSRIQQAMPEAEITPNVFISDKLTLKVTQVLSDALVVAARSLPEWCSRLVYKYPCLFTVETRNMYMQATAFGVSRTIVWLQQRRDAAVERARGSAQAGNSSAARQHDRYHEYRVGRLRHERVKVTRAEETLLDQAIRLMKFHADRKAVLEIEYTNEEGTGLGPTLEFYALVAAELQRKSLALWVCDDDDTHASKSGEEREVDLGEGKKPIGYYVRRVGGLFPAPLPPGTDETKRAADMFRVLGVFLAKVLLDGRLVDLPLSRPFLKLLVHPQIGDDARGPNLHKILSLDDFEEVNPVKGSFLKELRALAQRKRLIENDTSIDSNSKRRKIAELKLHIKGSTCRVEDLALNFTVNPPSKVFQYAEMELVDGGSDIDVTIDNVEQYVEKCEEFYLNTGIAYQMRAFRDGFDRVFPLRTLRAYSPEEVQRLLSGEQCPEWSRDDILNYTEPKLGYTRESPGFLRFVDVMEALTAQERKNFLQFATGCSSLPPGGLANLHPRLTIVRKVESGDGSYPSVNTCVHYLKLPEYSSSAILRERLLTAINEKGFHLN