Function
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
Sequence
MADSGNPNENTPSVATGENGEVTGSYNASAITVLEGLDAVRKRPGMYIGSTGERGLHHLVTEVVDNSVDEALAGHADTIDVTILADGGVRVVDNGRGIPVGIVPSEGKPAVEVVLTVLHAGGKFGGGGYSVSGGLHGVGVSVVNALSTKVAVEVKTDGYRWTQDYKLGVPTRRCAQNEATDETGTTVTFWADPDVFETTEYSFETLSRRFQEMAFLNKGLTLKLTDERESAKAVVGADVAGTDSAETPGEEPVRSVTYYYEGGIVDFVKYLNSRKGDLIHPTVIDIDAEDKERMLSVEIAMQWNSQYSEGVYSFANTIHTHEGGTHEEGFRAAMTGLVNRYAREKKFLREKDDNLAGEDIREGLTAIISVKLGEPQFEGQTKTKLGNTEAKTFVQKIVHEHLTDWFDRHPNEAADIIRKSIQAATARVAARKARDLTRRKGLLESASLPGKLSDCQSNDPSKCEIFIVEGDSAGGSAKSGRNPQYQAILPIRGKILNVEKARIDKILQNTEVQALISAFGTGVHEDFDIEKLRYHKIILMADADVDGQHINTLLLTFLFRFMRPLVEAGHVYLSRPPLYKIKWGRDDFEYAYSDRERDALVELGKQNGKRIKEDSIQRFKGLGEMNAEELRITTMDVDHRVLGQVTLDDAAQADDLFSVLMGEDVEARRSFIQRNAKDVRFLDI