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gumI

Gene
gumI
Protein
GDP-mannose:glycolipid 4-beta-D-mannosyltransferase
Organism
Xanthomonas campestris pv. campestris
Length
349 amino acids
Function
Nonprocessive beta-mannosyltransferase that catalyzes the transfer of a mannose residue from GDP-mannose to glucuronic acid-beta-1,2-mannose-alpha-1,3-glucose-beta-1,4-glucose-PP-polyisoprenyl to form the lipid-linked pentasaccharide repeating unit of xanthan, Man-GlcA-Man-Glc(2)-PP-Pol. Is involved in the biosynthesis of the exopolysaccharide xanthan. To a lesser extent, can also use ADP-Man and even GDP-Glc as sugar donor substrates in vitro. Is unable to transfer a Man residue to the free-tetrasaccharide GlcA-Man-Glc(2) used as an acceptor, which indicates that the diphosphate group and the lipid moiety in the acceptor substrate are of major importance for acceptor binding and catalysis.
Similarity
Belongs to the glycosyltransferase 94 family.
Mass
39.089 kDa
Sequence
MSASASLPVTRAAAAPRITVLFSTEKPNANTNPYLTQLYDALPDAVQPRFFSMREALLSRYDVLHLHWPEYLLRHPSKMGTLAKQACAALLLMKLQLTGTPVVRTLHNLAPHEDRGWRERALLRWIDQLTRRWIRINATTPVRPPFTDTILHGHYRDWFATMEQSTTLPGRLLHFGLIRPYKGVEVLLDVMRDVQDPRLSLRIVGNPATPXMRTLVETACAQDARISALLAYVEEPVLAREVSACELVVLPYKQMHNSGTLLLALSLARPVLAPWSESNAAIADEVGPGWVFLYEGEFDAALLSGMLDQVRAAPRGPAPDLSQRDWPRIGQLHYRTYLEALGKDGDAAL

Gene
gumI
Protein
GDP-mannose:glycolipid 4-beta-D-mannosyltransferase
Organism
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Length
349 amino acids
Function
Nonprocessive beta-mannosyltransferase that catalyzes the transfer of a mannose residue from GDP-mannose to glucuronic acid-beta-1,2-mannose-alpha-1,3-glucose-beta-1,4-glucose-PP-polyisoprenyl to form the lipid-linked pentasaccharide repeating unit of xanthan, Man-GlcA-Man-Glc(2)-PP-Pol. Is involved in the biosynthesis of the exopolysaccharide xanthan (By similarity).
Similarity
Belongs to the glycosyltransferase 94 family.
Mass
39.076 kDa
Sequence
MSASASLPVTRAAAAPRITVLFSTEKPNANTNPYLTQLYDALPDAVQPRFFSMREALLSRYDVLHLHWPEYLLRHPSKMGTLAKQACAALLLMKLQLTGTPVVRTLHNLAPHEDRGWRERALLRWIDQLTRRWIRINATTPVRPPFTDTILHGHYRDWFATMEQGTTVPGRLLHFGLIRPYKGVEVLLDVMRDVQDPRLSLRIVGNPATPQMRTLVETACAQDARISALLAYVEEPVLAREVSACELVVLPYKQMHNSGTLLLALSLARPVLAPWSESNAAIAEEVGPGWVFLYEGEFDAALLSGMLDQVRAAPRGPAPDLSQRDWPRIGQLHYRTYLEALGKDGDAAL