gtfA

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit

512 amino acids

Required for polymorphic O-glycosylation of the serine-rich repeat protein (SRRP) in this bacteria. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in the substrate protein. Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins encoded in the same operon. The GtfA-GtfB complex adds GlcNAc from UDP-GlcNAc to SRRP (experimentally characterized with a truncated SSR1 construct); the alpha linkage was shown for this enzyme but not the residues glycosylated on SRRP.

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

59.376 kDa

MTVYNINLGIGWASSGVEYAQSYRAQAFRNLNISAKFVFSDLILGNNIADLTANLGFNADQIIWLYNFFTDIKIAPSTFLLDTFVEQNHLEQRNFSLLPNNGTKELQYKSEEEKLTIVPRYNNREKQTIDQVTYIFNNRLIKRDFYSYTKYATEYYSGEEKDNQVIFREFYNENGTIAYTQHLDGDGHELFEFPDQNYYSKTDLYREMLRKFNFKADDIIILDRMDEDKQLVNGQLIFEHHLPAKLVIPVHADHYDKHYTNDHQVLWNNFYEYQFMHYQDVAAYVVATDRQRDLLASQQKHFNHAKPQINTIPVGSLEHLVKPKGTRKKHSLITASRLANEKHIDWVIEATVAAHKIVSDLTLDIYGEGGERSRLQNLITKNNADSYIKLMGQHDLKDVYQKYETYIAGSTSEGFGLSLMEAVGSGLSMIGFDVPYGNQTFIVDQQNGYLLPYTEDWSNSRKEQLLADAIVKNFTEADLTSFHEKSYSLAESYLTKNVAKQWQQLIGELQHA

gtfA

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit

506 amino acids

Required for polymorphic O-glycosylation of the serine-rich repeat protein Srr2. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues of Srr2. Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat proteins, probably Srr2 in this organism. The GtfA-GtfB (Gtf1-Gtf2 in this bacteria) complex adds GlcNAc from UDP-GlcNAc to Srr2 substrate. This subunit has low glycosyltransferase activity; GtfB enhances glycosyltransferase activity in vitro. Upon expression in S.parasanguis GtfA/GtfB restores expression of serine-rich repeat protein Fap1 and complements a biofilm formation defect.

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

58.196 kDa

MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQNENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKIISFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPCLYQRTFYNEDGSVAYEENVDGENSIFKFKETILYSKEELVGYMLEKLQLTNSDLILLDRSTGIGQAVLRNKGNAKVAVVVHAEHYNVSATDETTILWNNYYDYQFSNADSIDAFITSTETQTKTLIDQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKHIDWLINAVVEANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYKDYQVYLSGSTSEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNPQQISTAFAQYIVALFNSKDICKKHEYSYRIASRFLNDKIIENWSFFLRRLLNDYTI

gtfA

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit

506 amino acids

Required for polymorphic O-glycosylation of GspB, a serine-rich repeat cell wall protein encoded upstream in the same operon. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in GspB. Part of the accessory SecA2/SecY2 system specifically required to export GspB. Upon coexpression in E.coli with GtfB glycosylates GspB constructs. Glycosylation probably occurs intracellularly (PubMed:15489421). Requires GtfB for glycosylation activity, it has no activity alone. Does not use UDP-glucose as substrate. Has a fast, probably processive glycosylation phase followed by a slower, non-processive phase. The enzyme probably modifies its tertiary conformation by opening and closing its intersubunit interfaces to accomodate the increasingly glycosylated substrate; protein substrate recognition is provided by GtfB (PubMed:26884191).

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

58.313 kDa

MTVYNINLGIGWASSGVEYAQAYRAQILRRIQQPAKFIFMDMILADNIQHLTENIGFLDEEIIWLYNYFTDIKIAPTTVTLDQVLAQVAGQPERSEKEGKIVRYFYPQDDQFITCYLRQEDQDSVEHVEYVSRGRLIRKDYFSYVRYASEYFAPHNDAATLYQRRFYHEDGSVAYDMLIEDGQEKLYRFPDRIFYSKAELVRYFLQCLQLQADDVVILDRETGIGQVVFEESQKAKLGVVVHAEHFSENASSDDYILWNNFYDYQFTNADKVDFFIVATEAQKRILEQQFQHYSDKQPQIATIPVGSLDQLTYPKEPRKPYSMITASRLATEKHIDWLVAATVQAHAQLPELTLDIYGKGSEEDKLRRRIEEAGAQDYIRLKGHADLSQIYAGYELYLTASTSEGFGLTLMEAVGSGLPLIGFDVRYGNQTFIDDGKNGYLLPVSSNHVEDQIIAAFVEKIIALFSQGRQQEMSQHSYQVAENYLTSRVEAAWTQLLKEVRDDSAL

gtfA

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit

504 amino acids

Required for polymorphic O-glycosylation of serine-rich repeat protein Fap1. Catalyzes the first step in glycosylation by transfering N-acetylglucosamine from UDP-GlcNAc to serine residues in Fap1. Part of the accessory SecA2/SecY2 system specifically required to export Fap1, a serine-rich fimbrial adhesin encoded upstream in the same operon. The GtfA-GtfB (Gtf1-Gtf2 in this bacteria) complex adds GlcNAc from UDP-GlcNAc to Fap1, attaching the first sugar residue. Cannot use not UDP-Glc as substrate. This subunit has very low glycosyltransferase activity; the GtfB stabilizing protein enhances membrane association, protease resistance and glycosyltransferase activity.

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

58.239 kDa

MTIYNINLGIGWASSGVEYAQAYRAQILRSLGMPAKFIFTNMFQSENLEHFTKNIGFEDNEIIWLYGYFTDVKISGTTYKKDDLEATFSQCPTKKEASSDRKLIRYYFENQELYINASLYGENQEYVQRVEYVVKGKLIRKDYYSYTKVFSEFYSPGENGVQLCNRSFYNEDGSIAYEEILSNEKSTFVFSNKICYGLEELLEFMLEDLSLTKSDLILLDRATGIGQVVFENIGAAKLAVVIHAEHFNEKNTDEHNILWNNYYEYQFTNADKVNAFITSTERQKILLEEQFTQYTSLHPKIVAIPVGSLDQLKFPEQSRKSFSMMTGSRLAIEKHIDWLIEGVALAQKRLPELTFDIYGEGGERRKLTELLTKLHAGEFIELKGHKQLDEIYQNYELYLTASTSEGFGLTLMEAVGSGLPIIGFDVPYGNQTFVCSGENGLLIERPKGDDRSRIVQAFADSIYEYFTKFKMADAQQYSYNIAENYKHEKLVERWKDFIEEMLND

gtfA

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit

503 amino acids

Required for the polymorphic O-glycosylation of serine-rich repeat protein PsrP. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in PsrP (PubMed:24936067, PubMed:28246170). Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins encoded upstream in the same operon (Probable). The GtfA-GtfB complex adds GlcNAc from UDP-GlcNAc to PsrP (experimentally characterized with truncated PsrP-SSR1 constructs); this subunit alone has weak N-acetylglucosaminyl transferase activity that is 10-fold stimulated by GtfB. The complex requires at least a 25 residue-long peptide for activity; the in vitro assay has only been seen to glycosylate Ser residues (PubMed:24936067). The alpha linkage was shown in L.reuteri.

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

57.782 kDa

MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMILADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVLRVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAVLYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVILDRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFIVSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKHIDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYSQYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVEDQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD

gtfA

Sucrose phosphorylase

481 amino acids

Intracellular catabolism of sucrose. Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Belongs to the glycosyl hydrolase 13 family. Sucrose phosphorylase subfamily.

55.687 kDa

MPIINKTMLITYADSLGKNLKELNENIENYFGDAVGGVHLLPFFPSTGDRGFAPIDYHEVDSAFGDWDDVKCLGEKYYLMFDFMINHISRQSKYYKDYQEKHEASAYKDLFLNWDKFWPKNRPTQEDVDLIYKRKDRAPKQEIQFADGSVEHLWNTFGEEQIDLDVTKEVTMDFIRSTIENLAANGCDLIRLDAFAYAVKKLDTNDFFVEPEIWTLLDKVRDIAAVSGAEILPEIHEHYTIQFKIADHDYYVYDFALPMVTLYSLYSSKVDRLAKWLKMSPMKQFTTLDTHDGIGVVDVKDILTDEEITYTSNELYKVGANVNRKYSTAEYNNLDIYQINSTYYSALGDDDQKYFLARLIQAFAPGIPQVYYVGFLAGKNDLELLESTKEGRNINRHYYSSEEIAKEVKRPVVKALLNLFTYRNQSAAFDLDGRIEVETPNEATIVIERQNKDGSHIAKAEINLQDMTYRVTENDQTISFE

gtfA

dTDP-epi-vancosaminyltransferase

396 amino acids

Catalyzes the attachment of 4-epi-vancosamine from a TDP donor to the beta-OH-Tyr-6 of the aglycone cosubstrate in the biosynthesis of glycopeptide antibiotic chloroeremomycin, a member of the vancomycin group of antibiotics. Strongly prefers devancoaminyl-vancomycin (DVV) as substrate rather than the heptapeptide vancomycin aglycone (AGV). Acts downstream of GtfB.

Belongs to the glycosyltransferase 28 family.

41.662 kDa

MRVLITGCGSRGDTEPLVALAARLRELGADARMCLPPDYVERCAEVGVPMVPVGRAVRAGAREPGELPPGAAEVVTEVVAEWFDKVPAAIEGCDAVVTTGLLPAAVAVRSMAEKLGIPYRYTVLSPDHLPSEQSQAERDMYNQGADRLFGDAVNSHRASIGLPPVEHLYDYGYTDQPWLAADPVLSPLRPTDLGTVQTGAWILPDERPLSAELEAFLAAGSTPVYVGFGSSSRPATADAAKMAIKAVRASGRRIVLSRGWADLVLPDDGADCFVVGEVNLQELFGRVAAAIHHDSAGTTLLAMRAGIPQIVVRRVVDNVVEQAYHADRVAELGVGVAVDGPVPTIDSLSAALDTALAPEIRARATTVADTIRADGTTVAAQLLFDAVSLEKPTVPA