Function
A homolog of the beta sliding clamp protein encoded within the biosynthetic cluster for griselimycin synthesis (PubMed:26045430). Upon expression in S.coelicolor A3(2), which is susceptible to this antibiotic, confers resistance to griselimycin (PubMed:26045430). The beta sliding clamp confers DNA tethering and processivity to DNA polymerases and other proteins (By similarity). Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA (By similarity). Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity (By similarity). The beta chain is required for initiation of replication as well as for processivity of DNA replication (By similarity).
Sequence
MRFQVEREVLAEGIGWVARGLAVRPSVPILSGVVVNAEGDTLTLSGFDYEVSTRVELKANVEESGTVLIPGRRLADIAKVLPDVPIEFNVDQTKVYVQCDSNSFVLNALPLDEYPTLPKLPTVCGSVEGDQFARAVSQVAVVASRDDALPVLTGIGVNFDGEIMKLNATDRYRFAIRELAWKPEGTPSSSSVLVPARTLLDFAKSLNKGDLVKIALSDEGNLLGLHAGTRQMTCRLLEGTLPDYEKLFPKEFTSFGAVEVSRLVEALKRVSLVLERNSSVALDFTDGELVLQAGGADDDRATSRMAASLEGESIDIAFNPSFLLDGLTNLDASWAQFSFTSSNGKAVIMGKSSVDAEADTSARYLVMPVRFHR