Protein
Gluconate 5-dehydrogenase
Organism
Gluconobacter oxydans (strain 621H)
Function
Catalyzes the reversible NADP-dependent oxidation of gluconate to 5-ketogluconate. Is involved in the non-phosphorylative, ketogenic oxidation of glucose. Is almost inactive with NAD as cosubstrate. Displays high substrate specificity since D-Glucose, D-sorbitol, and D-mannitol are not oxidized by the enzyme, and 2-ketogluconate and L-sorbose are not reduced. Can accept D-fructose as a substrate, with a rate that is only 10% of the rate of 5-ketogluconate reduction.
Similarity
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Sequence
MSHPDLFSLSGARALVTGASRGIGLTLAKGLARYGAEVVLNGRNAESLDSAQSGFEAEGLKASTAVFDVTDQDAVIDGVAAIERDMGPIDILINNAGIQRRAPLEEFSRKDWDDLMSTNVNAVFFVGQAVARHMIPRGRGKIVNICSVQSELARPGIAPYTATKGAVKNLTKGMATDWGRHGLQINGLAPGYFATEMTERLVADEEFTDWLCKRTPAGRWGQVEELVGAAVFLSSRASSFVNGQVLMVDGGITVSL