Protein
Glycosyltransferase GlyD
Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Function
Also catalyzes the fourth step in glycosylation of PsrP in this bacteria. Can transfer the sugar from both UDP-glucose and UDP-galactose to the terminal sugar moiety of PsrP-GlcNAc-Glc-Glc and PsrP-GlcNAc-Glc-Gal; the C-terminal GT-D domain is also sufficient for this reaction in vitro (using truncated substrates with glycosylated PsrP SSR1). The N-terminal GT-D domain can transfer galactose from UDP-galactose to PsrP-GlcNAc-Glc-Gal or PsrP-GlcNAc-Glc-Glc in the fourth step.
Similarity
In the C-terminal section; belongs to the GT-D family.
Sequence
MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDWFRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLDSDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQSIVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAFNDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFSCLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLDKLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMIDTIKDFKKNGHLTCFRQLPSLTCLTFTASQYIEQLDYLAGQLPNVVFQIAAWTAMGPKLYDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFYKSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSSLVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTSWAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENRDLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHELEQEIEKYAGGRLILCMLGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEFIDDETYNSQIVARILN