gedh

Geraniol dehydrogenase

378 amino acids

Catalyzes the NAD(+)-dependent oxidation of geraniol to geranial, playing an important role in the biosynthesis of neral, an alarm pheromone. Cannot use NADP(+). Also acts as a farnesol dehydrogenase by catalyzing the oxidation of (2E,6E)-farnesol to (2E,6E)-farnesal, with lower activity compared to geraniol dehydrogenase activity.

Belongs to the zinc-containing alcohol dehydrogenase family.

40.63 kDa

VQNPGASAIQCRAAVLRKEGQPMKIEQVLIQAPGPNQVRVKMVSSGLCATDAHLVWGEQKISDLGGIGCPAIAGHEGAGIVESVGENVTEFVPGDSVLTSFQPQCGQCESCLRPSTNICKKYDLIKSTTDVSTARTLDGQPITSLFGLGVYSEYITTTEHHVFKVNKAANLEHASIISCSVGTGFYSATNLAAVYEGSTCAVWGLGGIGINTLFGCKYNKAKHIIGIDVNEDKREIAAEFGCTEFINPKTLGQPVEQYLMDKFGGVDFAFDCVGYKPILDQAAVSLAIDGTMVIIGAAAKEVKFEMPAFNFLFNRKVVGGLLGSKKTKVAYQELCDMYVDGTYDVDRLVSNKFSLDQINEAFQTLKDGNCIRSIVVFK

gedH

Anthrone oxygenase gedH

150 amino acids

Anthrone oxygenase; part of the gene cluster that mediates the biosynthesis of geodin, an intermediate in the biosynthesis of other natural products (PubMed:7665560, PubMed:19549600, PubMed:24009710). The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) gedC (PubMed:12536215, PubMed:19549600). The atrochrysone carboxyl ACP thioesterase gedB then breaks the thioester bond and releases the atrochrysone carboxylic acid from gedC (PubMed:19549600). The atrochrysone carboxylic acid is then converted to atrochrysone which is further transformed into emodin anthrone (PubMed:24009710). The next step is performed by the emodin anthrone oxygenase gedH that catalyzes the oxidation of emodinanthrone to emodin (PubMed:1810248). Emodin O-methyltransferase encoded probably by gedA then catalyzes methylation of the 8-hydroxy group of emodin to form questin (PubMed:1444712). Ring cleavage of questin by questin oxidase gedK leads to desmethylsulochrin via several intermediates including questin epoxide (PubMed:3182756). Another methylation step probably catalyzed by methyltransferase gedG leads to the formation of sulochrin which is further converted to dihydrogeodin by the sulochrin halogenase gedL (PubMed:24009710). Finally, the dihydrogeodin oxidase gedJ catalyzes the stereospecific phenol oxidative coupling reaction converting dihydrogeodin to geodin (PubMed:7665560).

Belongs to the anthrone oxygenase family.

16.271 kDa

MANPAVGAMMGLSLVAVPVFLDTNTQSEQLLAQFASLYDYGHKLMPTIAVATCALHGWVAARKRAAHQPWGRPVLAAVTTITMVPFTWVCMVSTNNALFQLHKGADANMEMVRALIARWQWLHVARSLFPLAGAIVACQTLLKELVGGSR