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ganA

Gene
ganA
Protein
Beta-galactosidase GanA
Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Length
673 amino acids
Function
Hydrolyzes oligosaccharides released by the endo-1,4-beta-galactosidase GalA from arabinogalactan type I, a pectic plant polysaccharide. It is unable to use lactose as a sole carbon source (By similarity). Catalyzes the hydrolysis of lactose to its constituent monosaccharides glucose and galactose. Possesses a low level of transgalactosylation activity for the production of galacto-oligosaccharides (GOS) from lactose.
Similarity
Belongs to the glycosyl hydrolase 42 family.
Mass
77.497 kDa
Sequence
MLHGGDYNPDQWLDRPDILADDIKLMKLAHTNTFSVGIFSWSALEPEEGVYTFEWLDDIFESIHRNGGRIILATPSGARPAWLSQKYPEVLRVNAERVKQLHGGRHNHCFTSYVYREKTKEINRMLAERYGSQHALLMWHVSNEYGGECHCDQCQHAFRDWLKKKYNHDIKSLNDAWWTPFWSHTFNDWSQIESPSPIGENAVHGLNLDWRRFVTDQTISFFQNEIVPLKEITPNIPITTNFMADTHDLIPFQGLDYSKFAKHLDVISWDAYPAWHNDWESTADLAMKVGFINDLYRSLKQQPFLLMESTPSAVNWHDFNKAKRPGMHLLSSVQMIAHGSDSILYFQWRKSRGSSEKFHGAVVGHDNCSENRVFKEVAKVGQTLEALSEVTGTIRPADVAILYDWENHWALQDAQGFGMKTKRYPQTLHEHYRAFWERDIPVDVITKEQDFSSYRLLIVPMLYLASEETIARLKAFAANGGTLVMTYISGIVNESDLTYLGGWPKDLQEMFGMEPVETDTLYPGDKNAVRYQNRSYELKDYATVLKLSTADPEGFYEDDFYADTTAVTSHPYKQGKTYYIGARLSSQFHRDFYGTLIKELAIQPALDVKHQPGVSVQVRQDEENDYIFIMNFTEKRQPVVLASAVKDMLTGETLAGEVTLEKYEARIAVKAKE

Gene
ganA
Protein
Beta-galactosidase GanA
Organism
Bacillus subtilis (strain 168)
Length
672 amino acids
Function
Hydrolyzes oligosaccharides released by the endo-1,4-beta-galactosidase GalA from arabinogalactan type I, a pectic plant polysaccharide. It is unable to use lactose as a sole carbon source. Maximal activity with o-nitrophenyl-beta-D-galactopyranoside (ONPG) and p-nitrophenyl-beta-D-galactopyranoside (PNPG) as substrates, trace activity with p-nitrophenyl-alpha-L-arabinopyranoside and o-nitrophenyl-beta-D-fucopyranoside as substrates, but no activity with p-nitrophenyl-alpha-D-galactopyranoside, p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-mannopyranoside or p-nitrophenyl-alpha-L-arabinofuranoside as substrates.
Similarity
Belongs to the glycosyl hydrolase 42 family.
Mass
77.499 kDa
Sequence
MLHGGDYNPDQWLDRPDILADDIKLMKLSHTNTFSVGIFAWSALEPEEGVYQFEWLDDIFERIHSIGGRVILATPSGARPAWLSQTYPEVLRVNASRVKQLHGGRHNHCLTSKVYREKTRHINRLLAERYGHHPALLMWHISNEYGGDCHCDLCQHAFREWLKSKYDNSLKTLNHAWWTPFWSHTFNDWSQIESPSPIGENGLHGLNLDWRRFVTDQTISFYENEIIPLKELTPDIPITTNFMADTPDLIPYQGLDYSKFAKHVDAISWDAYPVWHNDWESTADLAMKVGFINDLYRSLKQQPFLLMECTPSAVNWHNVNKAKRPGMNLLSSMQMIAHGSDSVLYFQYRKSRGSSEKLHGAVVDHDNSPKNRVFQEVAKVGETLERLSEVVGTKRPAQTAILYDWENHWALEDAQGFAKATKRYPQTLQQHYRTFWEHDIPVDVITKEQDFSPYKLLIVPMLYLISEDTVSRLKAFTADGGTLVMTYISGVVNEHDLTYTGGWHPDLQAIFGVEPLETDTLYPKDRNAVSYRSQIYEMKDYATVIDVKTASVEAVYQEDFYARTPAVTSHEYQQGKAYFIGARLEDQFQRDFYEGLITDLSLSPVFPVRHGKGVSVQARQDQDNDYIFVMNFTEEKQLVTFDQSVKDIMTGDILSGDLTMEKYEVRIVVNTH