Protein
FACT complex subunit spt16
Organism
Drosophila melanogaster
Function
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is required for expression of Hox genes.
Similarity
Belongs to the peptidase M24 family. SPT16 subfamily.
Sequence
MSSFVLDKEAFVRRVKRLYTEWRAPSIGHDDALRNLDCIMSIVGVEEDVMYSKSMALQLWLLGYELTDTISVFCSDAVYFLTSKKKIEFLKQTQNITEEGFPEINLLVRDRTDKDQGNFEKLIKALQNSKKGKRLGVFAKDAYPGEFSEAWKKSLTASKFEHVDISTIIAYLMCPKDESEINNIRKASLVSMDIFNKYLKDEIMDIIDSDRKVKHNKLSDGCEAAIGEKKYTSGLDPRLLDMAYPPIIQSGGAYSLKFSAVADKNPLHFGVIVCSLGARYKSYCSNISRTFLVNPTEAMQENYTFLVSVQEEILKLLVPGTKLCDVYEKTLDFVKKEKPSMVDNLPKSFGFAMGLEFRENSIVIGPKCQALLKKNMVFNLHVGISNLTNPEATDKEGKNYALFIGDTVLVGEQSPASVMTPSKKKIKNVGIFIKDDSDEEDVDDKKTAKEDQGTEILGRSKRNAVLESKLRNEINTEEKRKEHQRELAQQLNERAKDRLARQGNSKEVEKVRKNTVSYKSISQMPREPEVKELKLYVDKKYETVIMPVFGIQVPFHISTIKNISQSVEGEYTYLRINFFHPGATMGRNEGGLYPQPEATFVKEVTYRSSNVKEHGEVGAPSANLNNAFRLIKEVQKRFKTREAEEREKEDLVKQDTLILSQNKGNPKLKDLYIRPNIVTKRMTGSLEAHSNGFRYISVRGDKVDILYNNIKSAFFQPCDGEMIILLHFHLKYAIMFGKKKHVDVQFYTEVGEITTDLGKHQHMHDRDDLAAEQAERELRHKLKTAFKSFCEKVETMTKSVVEFDTPFRELGFPGAPFRSTVTLQPTSGSLVNLTEWPPFVITLDDVELVHFERVQFHLRNFDMIFVFKEYNKKVAMVNAIPMNMLDHVKEWLNSCDIRYSEGVQSLNWQKIMKTITDDPEGFFEQGGWTFLDPESGSEGENETAESEEDEAYNPTDAESDEESDEDSEYSEASEDSEESDEDLGSDEESGKDWSDLEREAAEEDRNHDYAADDKPRNGKFDSKKHGKSSKHSPSKSSKDKYNSRDKHHSSSSSGNKSSSKDKDRKRSRDDSRDNGHKSKKSRH