Protein
Diphthine methyl ester synthase
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Function
S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.
Similarity
Belongs to the diphthine synthase family.
Sequence
MLYLVGLGLSDETDITVKGLEVVKKAERVYLEAYTSILLVDQATLESYYGRPIVVADREMVESNSDEILRDADKVDVAFCVVGDPFGATTHTDLVLRARELGIQVRTVPNASIMSGIGAAGLQLYNFGQTVSMVFFLDNWRPASFYDRIKENRSIGLHTLVLLDIKVKEQSLENMARGRKIYEPPRYMTVGTCAQQMLEIEEEKQEGVYGPESLAIGCARVGGKTEKFVSGTLKELCDADDLLGPPLHSLILLGRRTHELEHDFVREFAVNKENWDRIWKAEYCGKQ