Protein
D-alanine--D-alanyl carrier protein ligase
Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Function
Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
Similarity
Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.
Sequence
MKLIETIKKYAQTQPDTLAFVNEEEKLTYGELWSQSERLAARIQSEALTDASPIIVYGHMKPVMAVSFLACVKAGHPYIPVDVSIPADRILKIINSSKAELLLNNSGTSVDTGDALISVVEPGVLEGDGVPETDPGRWVHGEDTFYIIYTSGSTGNPKGVQISADNLQSFTDWITNDFPVESGQVFLNQAPFSFDLSVMDLYPCLQSGGTLWTVTKDMINRPKLLFEALKQSNVNVWTSTPSFAQMCLMDPSYSEELLPELSLFMFCGETLPASVARQLKERFPKARVFNTYGPTEATVAVTSIEVTDDVLNKYSSLPVGSEKPETEIVIINEDGKAVQDGEKGEIIITGASVSKGYLGEKALTEKAFFSYNGSPAYRTGDAGYKENGQLFFLGRLDFQIKLHGYRIELEEIEYQINQSRYVQSAVVIPFYREEKIEYLIAMIVPAEHDFEKEYQLTSAIKKDLGSKLPAYMIPRKFMYQKEIPMTANGKIDRKRLKEEVTV