Protein
Processive diacylglycerol alpha-glucosyltransferase
Organism
Acholeplasma laidlawii
Function
Processive glucosyltransferase involved in the biosynthesis of both the non-bilayer-prone alpha-monoglucosyldiacylglycerol and the bilayer-forming membrane lipid alpha-diglucosyldiacylglycerol. These are major components for maintaining the anionic lipid surface charge density, for balancing the bilayer to non-bilayer phase equilibria and for keeping a constant lipid bilayer spontaneous curvature (curvature packing stress). Catalyzes the transfer of a glucosyl residue from UDP-Glc to diacylglycerol (DAG) acceptor to form the corresponding alpha-glucosyl-DAG (1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol), which then acts as acceptor to give alpha-diglucosyl-DAG product (3-O-(alpha-D-glucopyranosyl-alpha-(1->2)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). It can only use UDP-Glc as sugar donor.
Similarity
Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
Sequence
MKVLLYSQKQSMLKKSGIGRAFYHQKRALEAVGIEYTTDPKDTYDLVHVNIAHSNKIKKFRKKYPVIVHGHSTVQDFRRSFAFWRVIAPFFYKHLQNIYGIADLIITPTRYSKFLIESMHVVKSPVVALSNGIDLDAYEYKQENVDAFRKHFDLEPNQKVVIGVGLLFERKGIHDFIEVARTMPNVTFIWFGNLSKLATTHFIRKRIKNKPKNMIMPGYVDGAVIKGAFSGADCVFFPSYEETEGIVVLEGLASKTPVVLRDIPVYYDWLFHKEHVLKGHNNFEFSKLIEKVLHEDQTEMIENGYKIVQDRSIEKIGEGLKQAYQEVIKIKR