ddrA

Diol dehydratase-reactivating factor alpha subunit

610 amino acids

Alpha subunit of the diol dehydratase-reactivating factor (DDR) or DD reactivase. Contains the adenosine nucleotide binding site. DDR acts as a chaperone, reactivates the inactivated diol dehydratase holoenzyme in the presence of ATP, magnesium and free adenosylcobalamin (AdoCbl) by mediating the exchange of the tightly bound damaged cofactor AdoCbl for a free intact one. DDR has weak ATPase activity.

64.267 kDa

MRYIAGIDIGNSSTEVALATLDEAGALTITHSALAETTGIKGTLRNVFGIQEALALVARGAGIAVSDISLIRINEATPVIGDVAMETITETIITESTMIGHNPKTPGGAGLGTGITITPQELLTRPADAPYILVVSSAFDFADIASVINASLRAGYQITGVILQRDDGVLVSNRLEKPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIATVFNLSPEETKNIVPMARALIGNRSAVVVKTPSGDVKARAIPAGNLELLAQGRSVRVDVAAGAEAIMKAVDGCGRLDNVTGESGTNIGGMLEHVRQTMAELTNKPSSEIFIQDLLAVDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIAREIEQKLNIDVQIGGAEAEAAILGALTTPGTTRPLAILDLGAGSTDASIINPKGDIIATHLAGAGDMVTMIIARELGLEDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFSTPLPPAVFARVCVVKADELVPLPGDLALEKVRAIRRSAKERVFVTNALRALRQVSPTGNIRDIPFVVLVGGSSLDFEVPQLVTDALAHYRLVAGRGNIRGSEGPRNAVATGLILSWHKEFAHER

ddrA

Single-stranded DNA-binding protein DdrA

208 amino acids

ssDNA-binding protein that contributes to the ionizing radiation resistance of D.radiodurans. Plays a role in DNA repair and genome reconstitution, in a RecA-independent process, since DdrA is essential for recovery from severe genomic fragmentation as a result of exposure to severe levels of ionizing radiation in an environment lacking nutrients. In vitro, binds to the 3'-ends of single-stranded DNA, protecting them from nuclease degradation. Thus, DdrA is part of a DNA end-protection system that helps to preserve genome integrity following irradiation or desiccation. Does not display DNA strand annealing activity, unlike eukaryotic Rad52 protein homologs.

Belongs to the RAD52 family.

23.002 kDa

MKLSDVQKRLQAPFPAHTVSWKPAAFNAERTRALLLAHVDARAVQDRLDAVCPDDWSFEMEVVSGAEVPTVKGRLTVLGVTREDIGEAPEGSMAAYKAAASDAMKRCAVQFGIGRYLYDLPKQWADWDDARRGPKHLPELPEWARPDHERTPGGAHLVQAMEQLRYELPEDLDLQREVYKHLKAALGSIHPVPTGPVPTNPVQGGRAA

ddrA

Single-stranded DNA-binding protein DdrA

201 amino acids

ssDNA-binding protein that contributes to the ionizing radiation resistance of D.deserti. Plays a role in DNA repair and genome reconstitution, in a RecA-independent process, since DdrA is essential for recovery from severe genomic fragmentation as a result of exposure to severe levels of ionizing radiation in an environment lacking nutrients (Probable). In vitro, binds to the 3'-ends of single-stranded DNA, and probably protects them from nuclease degradation. Thus, DdrA is part of a DNA end-protection system that helps to preserve genome integrity following irradiation or desiccation.

Belongs to the RAD52 family.

22.224 kDa

MKLSDVQKRLQAPFPAHAVAWKPGVITKDRSRALMLAHIDARNVQDRLDAVCPDAWSFEVEVVPGTRLPTVKGRLTVLGVSREDIGEAPEGDLGTLKAAASDALKRCAVQFGIGRYLYDLPKQWVAWNDAKREPVSPPELPEWARPDHERSPGGAHLVQAMDQLRYEMPEDLELQREVYKHLKAALGSLHPISGGNQGRAA