Protein
Rhodomycin D methylesterase DauP
Organism
Streptomyces sp. (strain C5)
Function
Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. Catalyzes the removal of methyl group from the carbomethoxy group of rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 4-O-methylrhodomycin D to yield 10-carboxy-13-deoxycarminomycin and 10-carboxy-13-deoxydaunorubicin, respectively. Could be also involved in the decarboxylation of 10-carboxy-13-deoxycarminomycin and 10-carboxy-13-deoxydaunorubicin to yield 13-deoxycarminomycin and 13-deoxydaunorubicin, respectively. It seems that DauK may influence the ability of DauP to carry out the decarboxylation.
Similarity
Belongs to the methyl esterase DnrP family.
Sequence
MPTRMITKDEVTLWSEGIGDPADAPLLLIAGGNLSARSWPDEFVERLAAAGHFVIRYDHRDTGRSSRYDFALHPYGFDELATDALAVLDAWQVRAAHVVGMSLGNTIGQLLALDAPERLLTLTVMLGGALDVDFDADLEAALKGEPSVSGLPVPSRRFLDMMMLLQQPAGTDEELLERRVEKWRLLNGEGVPFDSDEFRRRELLAAGHAGTFDEPIVHHMIPQPPVSRGAELARITTPVLAIQAMCDPAAPPPHARHLADRIPGARVVEIENMGHALPLAVHEPLAAAICAHTRAATV