crmA

Gene

crmA

Protein

Isocyanide synthase-NRPS hybrid crmA

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)

Length

1537 amino acids

Function

Isocyanide synthase-NRPS hybrid; part of the crm gene cluster that mediates the biosynthesis of a yet unidentified copper-responsive metabolite.

Similarity

In the C-terminal section; belongs to the NRP synthase family.

Mass

170.24 kDa

Sequence

MFHKEAGISHLILDIILEHALNKFDPAPDRLQGAAKNFLPIIERFVAAGTRIEACLPAFPFKSANKVYKVLGSLPDKAEELALDRLNTMCARVREVYPPGLQVAIISDGITYNDTWAYGEALRQMAAQKQFMYIVFSRIKDLLDIPLPEQMSEIVYVANCTTFRRLLLNKYERADLDIDHEIASNPDTKLTYLGYRRFLESDLKYIFPRGAHRSAHSYKRDCKYLAKQMLIRGDAFAQAIKTSYPNHLRLSIHESVAGTKLSISLLNTKTGFTTPWHCSVAQLANGEWISAPMGEFRKDDRLELVYADGRPSHFREKPREGDAFGISESTASYLQRPKRLRASEYLGATLPSVPVSPGMSSPSAASTSSSGASMQGSAATTPETHSPPTFTWSGLEIVAEDNSNNASVPYGRRLIPQIMDSLAATEPERIVFSLATFSGDSLEFRPISARTFANAIDKTAWWLHNQVGRPDSIQPVGYIGPHDLRHVLLTYACVKAGYAALFLSPKNSTEGALAVLEATKCDLWINACDVSPVPLVKEVLQKRPMNVLQLPQLDELLDAVSTDPFPYTKKFDEAINDPFCFLHTSGTTGVPKPIPWSHGLIGTMDAVRLLPPGADGDLPPWTTDWKTGDTIYSSFPMSHGAGIIMNILMPALFNLHCVFGPAGVLPNINLVDALAVSTRIDIWSMVPSLVDELGETPAVLSKLKSSKFICASGGPVSPVSAGKVNEVIRVLNLTGTTEGLFMGNLIPPREDWFWFCFHPYSGFEFKQVEPDTYEHWVHRNEHWPLFQGIFHTFPEKQSINFKDLYVRHPTKPNLWAFKGRSDDLVTEAFITTHPAIKGCLVFGTGKPQAGLLIELKDPLQKTDELLDSIWETVQQANSMSRHKNQLLRDFVAFATPDKPFCRTDKGTVKRSATLKLYADYIERFYRSRNDDLGGTFDFDMSSAHSIEDNVRKILAASLPDVQEASADTDLFALGLDSLGVFAAIKTIRAATGLGDQIGPRHIYANPTIARLSAIIALLAAAAESASDTTLCERPVDDVGAQIARMIAQHKARQSFSLNAFDYVNPNHGMGLVLYFPIRDGVSYEQVFANLQAGLNRTFDLIPALSGKMTDCSEQGIGYTKGDLCVTIPPLAKADSARNRLVYKDLSAVLPSFDDLRKGGFAPSAFSDTLANFVSGGCILAVDLNHCCLDGLGAMVALKAWAENCRYLQGDQSATCGWYDPESFNHSLPEILHRQEGWARPLHEIDPGTWGFLPFFPPEDEETNPRCEKATEGSLPARPIFPLHPVWPLPRAERCLKTTMFLVTPEKLELLQQDVIADPATNGITPSISDIVQAFFWRAAIKARYRVATEIRKQKFSPDAVSILELPTDTRPHFSSRLPPTYMGSMLILNRTSMPIETLCSAETSIAKVALLLRQSAARITPSLVHDAFTLLQSLPGHRRFSTANMGLEHMHAMISNMLLFPTSEIGFGDAFFANGGVPETMRPQLERGNGRFRFLAVFPLRKDGGVELVLGTHREELEMLVTDEEFTRYARMVDTCC

Protein

Isocyanide synthase-NRPS hybrid crmA: crmA

Gene

CRMA

Protein

Serine proteinase inhibitor 2

Organism

Cowpox virus (strain Brighton Red)

Length

341 amino acids

Function

Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a cross-class interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.

Similarity

Belongs to the serpin family. Poxviruses subfamily.

Mass

38.077 kDa

Sequence

MDIFREIASSMKGENVFISPPSISSVLTILYYGANGSTAEQLSKYVEKEADKNKDDISFKSMNKVYGRYSAVFKDSFLRKIGDNFQTVDFTDCRTVDAINKCVDIFTEGKINPLLDEPLSPDTCLLAISAVYFKAKWLMPFEKEFTSDYPFYVSPTEMVDVSMMSMYGEAFNHASVKESFGNFSIIELPYVGDTSMVVILPDNIDGLESIEQNLTDTNFKKWCDSMDAMFIDVHIPKFKVTGSYNLVDALVKLGLTEVFGSTGDYSNMCNSDVSVDAMIHKTYIDVNEEYTEAAAATCALVADCASTVTNEFCADHPFIYVIRHVDGKILFVGRYCSPTTN

Protein

Serine proteinase inhibitor 2: CRMA