About Products Protein Database Contact

cntM

Gene
cntM
Protein
Pseudopaline synthase
Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Length
433 amino acids
Function
Catalyzes the NADH-dependent reductive condensation of alpha-ketoglutarate to the intermediate formed by the adjacently encoded enzyme CntL, namely (2S)-2-amino-4-{[(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate, leading to the production of pseudopaline. This is the last step in the biosynthesis of the metallophore pseudopaline, which is involved in the aquisition of nickel and zinc, and thus enables bacterial growth inside the host, where metal access is limited. Therefore, this enzyme probably contributes to Pseudomonas virulence. Can use neither pyruvate nor NADPH in place of alpha-ketoglutarate and NADH, respectively.
Similarity
Belongs to the staphylopine dehydrogenase family.
Mass
47.699 kDa
Sequence
MNAADESLGNVLLVGLGAVAIQVALDLRRHGAGRLGALNHPGRRSQRIAEALARGACLQLEGQGQHRWLSGNAALDVFHQDPAELRDDWQTLVLCVPADSYLDVVRGLPWERLGGVRTLLLVSAFIGANLLVRSALPAGCQATVLSLSSYYAATKVIDETQPLRALTKAVKRRVYLGSSRPDCPARETWRRVLAGSGVEVVPLATPEAAEGRNVTTYVHSPFFLGEFALARILSEQGPPGFMYKLYPEGPITPGAIGAMRRLWCELSELLRRMGAEPLNLLRFLNDDNYPVHETMLPRAAIDGFAEAGAERQEYLLFVRYAALLVDPFSPADEQGRHFDFSAVPFRRVSRDEDGLWRLPRVPLEDYRKLALIVALAAHFDLAMPQARSLLASYENAVSRFIDCQGASQCHPSLYPIDSRPAADAIYRQWCSTC

Gene
cntM
Protein
Pseudopaline synthase
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Length
433 amino acids
Function
Catalyzes the NAD(P)H-dependent reductive condensation of alpha-ketoglutarate to the intermediate formed by the adjacently encoded enzyme PA4836, namely (2S)-2-amino-4-{[(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate, leading to the production of pseudopaline. This is the last step in the biosynthesis of the metallophore pseudopaline, which is involved in the aquisition of nickel and zinc, and thus enables bacterial growth inside the host, where metal access is limited. Therefore, this enzyme probably contributes to Pseudomonas virulence. Can use neither pyruvate nor oxaloacetate in place of alpha-ketoglutarate (PubMed:29091735, PubMed:29618515). Is two-fold more efficient using NADPH than NADH as the electron donor (PubMed:29618515).
Similarity
Belongs to the staphylopine dehydrogenase family.
Mass
47.715 kDa
Sequence
MNAADESLGNVLLVGLGAVAIQVALDLRRHGAGRLGALNHPGRRSQRIAEALARGACLQLEGQGQHRWLSGNAALDVFHQDPAELRDDWQTLVLCVPADSYLDVVRGLPWERLGGVRTLLLVSAFIGANLLVRSALPAGCQATVLSLSSYYAATKVIDETQPLRALTKAVKRRVYLGSSRPDCPARETWRRVLAGSGVEVVPLATPEAAEGRNVTTYVHSPFFLGEFALARILSEQGPPGFMYKLYPEGPITPGAIGAMRRLWCELSELLRRMGAEPLNLLRFLNDDNYPVHETMLPRASIDGFAEAGAERQEYLLFVRYAALLVDPFSPADEQGRHFDFSAVPFRRVSRDEDGLWRLPRVPLEDYRKLALIVALAAHFDLAMPQARSLLASYENAVSRFIDCQGASQCHPSLYPIDSRPAADAIYRQWCSTC

Gene
cntM
Protein
Staphylopine synthase
Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Length
433 amino acids
Function
Catalyzes the NADPH-dependent reductive condensation of pyruvate to the intermediate formed by the adjacently encoded enzyme CntL, namely (2S)-2-amino-4-{[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate, leading to the production of staphylopine. This is the last step in the biosynthesis of the metallophore staphylopine, which is involved in the acquisition of nickel, cobalt, zinc, copper, and iron, and thus enables bacterial growth inside the host, where metal access is limited. Therefore, this enzyme probably contributes to staphylococcal virulence. Can use neither NADH nor alpha-ketoglutarate in place of NADPH and pyruvate, respectively.
Similarity
Belongs to the staphylopine dehydrogenase family.
Mass
50.072 kDa
Sequence
MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLYQAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQQLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRIVDKEAPNHVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPPLFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVNLLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHYFDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEASCQAYKDMHQDQQLSSQFNTNLFEGDKALVTKFLEINRTLS