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cntL

Gene
cntL
Protein
D-histidine 2-aminobutanoyltransferase
Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Length
272 amino acids
Function
Catalyzes the nucleophilic attack of one alpha-aminobutanoate moiety from SAM onto D-histidine to produce the intermediate (2S)-2-amino-4-{[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate. Functions in the biosynthesis of the metallophore staphylopine, which is involved in the acquisition of nickel, cobalt, zinc, copper, and iron, and thus enables bacterial growth inside the host, where metal access is limited. Therefore, this enzyme probably contributes to staphylococcal virulence (PubMed:27230378, PubMed:29091735). Appears to be specific for D-histidine as substrate (PubMed:29091735).
Similarity
Belongs to the methyltransferase superfamily. CntL family.
Mass
31.126 kDa
Sequence
MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYSEFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYFDNIEHCIDEEFGQCSITSNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLGRRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVVAMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV

Gene
cntL
Protein
L-histidine 2-aminobutanoyltransferase
Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Length
263 amino acids
Function
Catalyzes the nucleophilic attack of one alpha-aminobutanoate moiety from SAM onto L-histidine to produce the intermediate (2S)-2-amino-4-{[(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate. Functions in the biosynthesis of the metallophore pseudopaline, which is involved in the aquisition of nickel and zinc, and thus enables bacterial growth inside the host, where metal access is limited. Therefore, this enzyme probably contributes to Pseudomonas virulence. Cannot use D-histidine in place of L-histidine as substrate.
Similarity
Belongs to the methyltransferase superfamily. CntL family.
Mass
28.932 kDa
Sequence
MQGRTPLLETLRELECEIRLLTVYARECCGCYEILRRKLDRLSGLIGEDCSRAQWQADSDDPALQALGLRLRDAAVQALCELEKHLCQGVLHEPGEMGRYLGSLLESIRGELDSAGIDADARVLFVGSGALPTSALVLAREVGAHLCCLDIDEEALGYAREIARCQGLEARMQFSSLPPAELAFSRDATHFLIASLVQQKSAVLAQIRQVMRADAKVLLRHGSGIKGLFNYPVEPAELEGWQVCAERVSQPLYDTLILEKAGR

Gene
cntL
Protein
L-histidine 2-aminobutanoyltransferase
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Length
263 amino acids
Function
Catalyzes the nucleophilic attack of one alpha-aminobutanoate moiety from SAM onto L-histidine to produce the intermediate (2S)-2-amino-4-{[(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate. Functions in the biosynthesis of the metallophore pseudopaline, which is involved in the aquisition of nickel and zinc, and thus enables bacterial growth inside the host, where metal access is limited. Therefore, this enzyme probably contributes to Pseudomonas virulence. Appears to be specific for L-histidine as substrate.
Similarity
Belongs to the methyltransferase superfamily. CntL family.
Mass
28.886 kDa
Sequence
MQGRTPLLETLRELECEIRLLTVYARECCGCYEILRRKLDRLSGLIGEDCSRAQWQADSDDPALQALGLRLRDAAVQALCELEKHLCQGVLHEPGEMGRYLGSLLESIRGELDSAGIDADARVLFVGSGALPTSALVLAREVGAHLCCLDIDEEALGCAREIARCQGLEARMQFSSLPPAELAFSRDATHFLIASLVQQKSAVLAQIRQVMRADAKVLLRHGSGIKGLFNYPVEPAELDGWRVCAERVSQPLYDTLILEKAGR