cmlA

Gene

cmlA

Protein

4-amino-L-phenylalanyl-[CmlP-peptidyl-carrier-protein] 3-hydroxylase

Organism

Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)

Length

532 amino acids

Function

Involved in chloramphenicol biosynthesis (PubMed:20713732). Catalyzes the beta-hydroxylation of 4-amino-L-phenylalanine (L-PAPA) covalently bound to CmlP to form L-p-aminophenylserine (PubMed:20713732).

Similarity

Belongs to the metallo-beta-lactamase superfamily.

Mass

60.171 kDa

Sequence

MRYSLRQDIAVEPVIAGWYGWSYLLPPQTLARFVHNRFNRIVESYLDDPQVHAAAVRQRRMHGGPWIHAHEHRDAIEAWYRETAPRRERLDELFEAVRRLEEDILPRHHGECLDPVYQELPAALAGRVEVFYGRDNRTADYRFVEPLMYASEYYDESWQQVRFRPVTEDAREFALTTPMLEYGPEQLLVDVPLNSPLLDAVFRGGLTGTELDDLAARFGLDGERAARFASYFEPTPAASEAPAPASSSEEDVLEYVGHACVFARHRGTTFLVDPVLSYSGYPGGAENRFTFADLPERIDHLLITHNHQDHMLFETLLRIRHRVGRVLVPKSTNASLVDPGLGGILRRLGFTDVVEVDDLETLSCGSAEVVALPFLGEHGDLRIRSKTGWLIRFGERSVLFAADSTNISPTMYTKVAEVIGPVDTVFIGMESIGAAASWIYGPLYGEPLDRRTDQSRRLNGSNFPQAREIVDALEPDEVYVYAMGLEPWMGVVMAVDYDESHPAIVDSDLLVRHVQDKGGTAERLHLRRTLRL

Protein

4-amino-L-phenylalanyl-[CmlP-peptidyl-carrier-protein] 3-hydroxylase: cmlA

Gene

cmlA

Protein

Chloramphenicol resistance protein

Organism

Pseudomonas aeruginosa

Length

419 amino acids

Function

Appears to provoke a reduction of the content of the major porins OmpA and OmpC.

Similarity

Belongs to the major facilitator superfamily. Bcr/CmlA family.

Mass

44.316 kDa

Sequence

MSSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNALGTTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSAEVFLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGALVDMWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLYTLCYAAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKWGSPSVLRMGMGCLIAGAVLLAITEIWALQSVLGFIAPMWLVGIGVATAVSVAPNGALRGFDHVAGTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVSRVKGSRGQGEHDVVALQSAESTSNPNR

Protein

Chloramphenicol resistance protein: cmlA

Gene

cmlA

Protein

Chloramphenicol acetyltransferase 2

Organism

Escherichia coli

Length

213 amino acids

Function

This enzyme is an effector of chloramphenicol resistance in bacteria.

Similarity

Belongs to the chloramphenicol acetyltransferase family.

Mass

24.778 kDa

Sequence

MNFTRIDLNTWNRREHFALYRQQIKCGFSLTTKLDITALRTALAETGYKFYPLMIYLISRAVNQFPEFRMALKDNELIYWDQSDPVFTVFHKETETFSALSCRYFPDLSEFMAGYNAVTAEYQHDTRLFPQGNLPENHLNISSLPWVSFDGFNLNITGNDDYFAPVFTMAKFQQEGDRVLLPVSVQVHHAVCDGFHAARFINTLQLMCDNILK

Protein

Chloramphenicol acetyltransferase 2: cmlA

Gene

cmlA

Protein

Chloramphenicol acetyltransferase 2

Organism

Escherichia coli

Length

213 amino acids

Function

This enzyme is an effector of chloramphenicol resistance in bacteria.

Similarity

Belongs to the chloramphenicol acetyltransferase family.

Mass

24.778 kDa

Sequence

Protein

Chloramphenicol acetyltransferase 2: cmlA