cas3

CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST

1098 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Might be involved in the integration of spacer DNA into the CRISPR cassette.

In the central section; belongs to the CRISPR-associated helicase Cas3 family.

124.892 kDa

MNILLISECNKRALVETRRILDQFAERKGERSWQTAITLEGLNTLRKLLRKTARRNTAVACHWIRSTNHTELLWVVGNLRRFNAQGSVPTNTTSRDVLRTKDENPWHSAEVFSLLAAIAGLFHDVGKANALFQAGLSGTGPRSQPYRHEWVSLRLFQAFVGEQDDKAWLTTLSMITSEAEVALLATLQQDKPTFSDSPFRTLPPLAQTIAWLIVSHHRLPVFNKSTELAPNSSEPQLSFAETWLTDHLSPQWNALNHCQIGWTPHEREQNWQFPNGTPLYSAVWREKARKFAGRALKLPSFMHFSQLDQRLTVHLARLALMLADHHYSAGAATVGWQDITYPVWANTDRKTGEYKQRLDEHCVGVGQNALLLGRSLPHLRDTLPAITRHKGFRQRSTHPRFRWQDRAFDLACSIRETSKQHGFFGINMASTGRGKTFANARIMYGLSDESTGCRFSVALGLRTLTLQTGDALRQRLKLDEDDLAVLIGSQAVQDLHEMRKENQQRQQNTPQTGSESADPLFSEHQYVRYDGSLDDGRLKAWLERSPTLHQLLSAPVLITTIDHLMPATEALRGGHQIAPMLRLLTADLVLDEPDDFGLEDLPALCRLVNWAGMLGSRVLLSSATLPPALIRALFDAYLDGRTAWQQAYGTPNTPLNVCCGWFDEFDCQHEQYGDVKDFMVRHDAFVHQRLKNLTKDELPLRFATIVPVSSPSKNADDVYLAVAQAIHPRMFDLHSQHHQQHENGKTVSLGLVRMANIDPLVAVARQLLAIPSPPDTCIHYCIYHSQHPLAMRSHIEQRLDAALMRNDADALWQVEEIRQAIEKSSQRHHVFVALATSVAEVGRDHDYDWAIVEPSSMRSLIQPAGRILRHRQDKQYVPKTPNIYLLSHNIRALRGKDIAYCKPGFESQDDSLDTHDLHQLLQEKEYRHLSAAPRIVQPMSFAKPLSLVALEHAVLGKTLLGLKNKKLDDLKRPPAAFWWRAHPHWNGELQRRTPFRQSAKDEAYTLWIADDDEEPVFMVQDDGPSGWKQSDIARPVTLNMAEGVSAWIEPDYHALYQQLAEEKQWELSWVSARFGEIRLREEEDWYWHPLLGVFGALS

cas3

CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST

1076 amino acids

In this bacteria Y.pestis-subtype CRISPRs do not confer resistance to phage DSM3 or MP22, but instead are required for DMS3-dependent inhibition of biofilm formation and possibly motility.

In the central section; belongs to the CRISPR-associated helicase Cas3 family.

121.091 kDa

MNILLVSQCEKRALSETRRILDQFAERRGERTWQTPITQAGLDTLRRLLKKSARRNTAVACHWIRGRDHSELLWIVGDASRFNAQGAVPTNRTCRDILRKEDENDWHSAEDIRLLTVMAALFHDIGKASQAFQAKLRNRGKPMADAYRHEWVSLRLFEAFVGPGSSDEDWLRRLADKRETGDAWLSQLARDDRQSAPPGPFQKSRLPPLAQAVGWLIVSHHRLPNGDHRGSASLARLPAPIQSQWCGARDADAKEKAACWQFPHGLPFASAHWRARTALCAQSMLERPGLLARGPALLHDSYVMHVSRLILMLADHHYSSLPADSRLGDPNFPLHANTDRDSGKLKQRLDEHLLGVALHSRKLAGTLPRLERQLPRLARHKGFTRRVEQPRFRWQDKAYDCAMACREQAMEHGFFGLNLASTGCGKTLANGRILYALADPQRGARFSIALGLRSLTLQTGQAYRERLGLGDDDLAILVGGSAARELFEKQQERLERSGSESAQELLAENSHVHFAGTLEDGPLREWLGRNSAGNRLLQAPILACTIDHLMPASESLRGGHQIAPLLRLMTSDLVLDEVDDFDIDDLPALSRLVHWAGLFGSRVLLSSATLPPALVQGLFEAYRSGREIFQRHRGAPGRATEIRCAWFDEFSSQSSAHGAVTSFSEAHATFVAQRLAKLEQLPPRRQAQLCTVHAAGEARPALCRELAGQMNTWMADLHRCHHTEHQGRRISFGLLRLANIEPLIELAQAILAQGAPEGLHVHLCVYHSRHPLLVRSAIERQLDELLKRSDDDAAALFARPTLAKALQASTERDHLFVVLASPVAEVGRDHDYDWAIVEPSSMRSIIQLAGRIRRHRSGFSGEANLYLLSRNIRSLEGQNPAFQRPGFETPDFPLDSHDLHDLLDPALLARIDASPRIVEPFPLFPRSRLVDLEHRRLRALMLADDPPSSLLGVPLWWQTPASLSGALQTSQPFRAGAKERCYALLPDEDDEERLHFSRYEEGTWSNQDNLLRNLDLTYGPRIQTWGTVNYREELVAMAGREDLDLRQCAMRYGEVRLRENTQGWSYHPYLGFKKYN

cas3

CRISPR-associated nuclease/helicase Cas3

937 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity (By similarity). Plasmid targeted by CRISPR locus P1 transform wild-type cells very poorly (PubMed:22767603).

In the central section; belongs to the CRISPR-associated helicase Cas3 family.

103.679 kDa

MTYPLISHPEANDGERTYPDEQLTDDGSLRLTAHNTVVSEYATRLFRGPETQRRFLCVAASLHDFGKATPQFQAYVRDEYEGPEKEKNHARLGALATWFVLDQRDAPARDKLAATLAVARHHQALPDAAQYTAESLADAFETSNSVLTAQLEAISERWPQKATELLQRSGETDSTWDEFRTWVQSGTVVGELHEVSARRELTGPKATSDKLPRKLYDRTLHYWAAITLADKSHAMAVPESHVFDVETLDRETLEEYISGLRAEPPDDELERALNDERERARRQAIDGVHEWLGGDAQTPPIATLTLPTGLGKTFTGLSAAFEARGILESDGGPTRPIVYALPYTSIIEQTRSIFERPELWGADPTKSALTVHHYLSETVVYRNERESEDVASTDQEEHASFLGEAWRDGTVLTTFVQLFESLVGPSNRQGLKLSALDDGLVILDEPQALPKEWWDGITRLIELLTTEYQTRVIAMTATQPTLLRDVETTSLLDVGREHDKTGCLRCEVGPDYPVRLEPARKETYFENAERVRYRIDESALSFHLSADERYLSHETAAARVVAATAAGEGGSTLAICNTINSSATLTQELCGHDGVTHLGEAIDSVLGANDVDATKQENNVSAIVDAILRRSGLRGVDGELSVPAGTDIVVATLNSRYRPFDRRLLIEIADTLSGSEIPFVLISTQAIEAGVDLSFKRVFRDIAPLDSIVQAGGRCNRSYEWGRNGGQVVVWTLADPDEENPGDPTKSPPAHWVYERGSSDAGIQTHLQLISSILAQFEDEEVPDAEISHHAVNEYFDALREKSLSSTKIRTLIDDAKAGKLARESLIGGYQTVDVLVATTQAERKRLNELTELFTSDDPTDRSTGYKKLEQAAGIRVSLPLNSIEQLPDVSRVDGKERNEDGVQVFRYTGTESLEYDLQTGGLRGKEDTVAGRFTTF

cas3

CRISPR-associated nuclease/helicase Cas3

926 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Functions as a ssDNA-dependent ATPase; dsDNA, ssRNA do not stimulate ATPase activity, while other nucleotides (aside from dATP) are not hydrolyzed. Functions as a ssDNA nuclease; activity does not require ATP. Functions as an ATP-dependent helicase; helicase activity requires hydrolysable ATP. Unwinds both DNA/DNA hybrids and RNA/DNA hybrids, moving mostly in a 3' to 5' direction.

In the central section; belongs to the CRISPR-associated helicase Cas3 family.

105.76 kDa

MKHINDYFWAKKTEENSRLLWLPLTQHLEDTKNIAGLLWEHWLSEGQKVLIENSINVKSNIENQGKRLAQFLGAVHDIGKATPAFQTQKGYANSVDLDIQLLEKLERAGFSGISSLQLASPKKSHHSIAGQYLLSHYGVDEDIATIIGGHHGRPVDDLDGLNSQKSYPSNYYQDEKKDSLVYQKWKSNQEAFLNWALTETGFNSVSQLPKIKQPAQVILSGLLIMSDWIASNEHFFPLLSLDETDVKNKSQRIETGFKKWKKSNLWQPETFVDLVTLYQERFGFSPRNFQLILSQTIEKTTNPGIVILEAPMGIGKTEAALAVSEQLSSKKGCSGLFFGLPTQATSNGIFKRIEQWTENIKGNNSDHFSIQLVHGKAALNTDFIELLKGNTINMDDSENGSIFVNEWFSGRKTSALDDFVVGTVDQFLMVALKQKHLALRHLGFSKKVIVIDEVHAYDAYMSQYLLEAIRWMGAYGVPVIILSATLPAQQREKLIKSYMAGMGVKWRDIENIDQIKIDAYPLITYNDGPDIHQVKMFEKQEQKNIYIHRLPEEQLFDIVKEGLDNGGVVGIIVNTVRKSQELARNFSDIFGDDMVDLLHSNFIATERIRKEKDLLQEIGKKAIRPPKKIIIGTQVLEQSLDIDFDVLISDLAPMDLLIQRIGRLHRHKIKRPQKHEVARFYVLGTFEEFDFDEGTRLVYGDYLLARTQYFLPDKIRLPDDISPLVQKVYNSDLTITFPKPELHKKYLDAKIEHDDKIKNKETKAKSYRIANPVLKKSRVRTNSLIGWLKNLHPNDSEEKAYAQVRDIEDTVEVIALKKISDGYGLFIENKDISQNITDPIIAKKVAQNTLRLPMSLSKAYNIDQTINELERYNNSHLSQWQNSSWLKGSLGIIFDKNNEFILNGFKLLYDEKYGVTIERLDKNESV

cas3

CRISPR-associated endonuclease/helicase Cas3

920 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. The N-terminal domain (residues 6-260) acts as a ssDNA endonuclease, has no activity on dsDNA.

In the central section; belongs to the CRISPR-associated helicase Cas3 family.

102.127 kDa

MSVEEAALALWAKSGNPFHPLLAHMLDTAAVALAVLRMEPPRTRALYAEDWGLPEEGALAWAAALVGLHDLGKASPVFQAGWEEGKERVQRAGLPFGELLDWVAHGVFTELFLRRLLKEKGLPERAANDLAAALGAHHGFPANAEEKSRARRHLRTEDPLWKEARRWLLEEVFRRLGAPLPPSQGNGEARPEAVLRVMALASFADWVASDPSLFPYGRDPRRGDYLKEALRLAQEALNRLGWPAFAKAQRREFGELFPYIPKPNALQESVPALLEGACTPVLLLVEAPMGMGKTEAALYAHHLLQAGLGHRGLYVALPTQATANGLFPRVRGFLERLGEGSRLELQLQHGTALLNPHYAGLLERAAPRQVGEEEEGGAVASAWFSARKRAMLAPYGVGTLDQALLGVLRVKHHFVRLWGLMNRVVVLDEVHAYDVYTSGLLQALLRWLRALGSSAVVMTATLPPSRRRALLEAWAGEEVEGQDLGPYPRVVLVGEGVKARSLPPAREVEVALEVLREVDVEPLAQRLKGALPGAVGAIVNTVDRAQDLYRALGEGTPLTLEELARRLGGISGGQAWEEVRQALPERGGEVVGKVLTDGTLVFLLHARFPAEERALRGSVVLALFGKGGPRPPRAILVATQVAEQSLDLDFDLLYTDLAPIDLLFQRSGRLHRHERPRPEEHARPRLLLGVPEDLDFGKPLYWDKVYEDYVLLATWRALSGRDRLRVPGDLEALLEEIYEGENPESFPEGLRERAKKSLKALQERRDREANTARRLSLSELDRLLAYWDEGALVAQERLEDDEEKAETQRLLTRLGDPSVAVVPLFRVGEGLFLDREGRRRAPLKGEVSREEAEALFRRAVRLSRFPLPQELLKEEPPPAWRKSGLLRGLRPLEVGRVFRSGERAFQVELDPELGVVYLPV

cas3

CRISPR-associated nuclease/helicase Cas3

830 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Anneals and unwinds R-loops (in which crRNA binds the target DNA, displacing the noncomplementary strand). Unwinding requires ATP, annealing does not.

In the central section; belongs to the CRISPR-associated helicase Cas3 family.

96.329 kDa

MAMMELIDIIRAKSNDQRNYLLKDHLKETVARIDDFHNFYQTNREKFSYKIGEKTFRALAMASIIHDLGKIDYNFQKKLMGDDSEAWEVLEEFLSPLKPLKRSPRHEILSIIWSTFLLGNDDLDAMMRTAILLHHYNEYFLNDKDLMEIIFTYRDAFEIYLNFIIEKKETLRIFIEDILNYIQNSLESDLVISAADEIRSNMDFEKPELLLEKIKEYDDDISEFAAFYEPEERSADILILSGILRRADYSASAGVDIELFSEEVFRDIDEKITSKIGGAPWQIRLMGELGGPKKMVLVAPTGSGKTEFSILWAAKHGRKFIYTLPLRVALNDIFMRLRDSDGYFSEDEIDILHSTAFIEYLKEERLGRGTDLDSMMTSARLMASPALLTTPDQVLITSLNYFGSDKVISVYPFASMILDEIQTYNEEMAAVIIKTLELVNEVDGNILVMTATLPPYFRSFLDAMNFEVMDVAAIPGAHDIKNLNLKRHVPQLIEEPLFNDELEVSDKLGKILDENSEKNVLIVVNNVQKAIELYREYQDDPDVYLLHSRLLEKVKSQRIGEVKKRSQDERGLTVISTQIIEASVDIDFDLMITEISTIDSQIQRWGRIHRNRDADYDSGDPNIIIFTDSDRRTSLIYDKKVLDATRAILERYDGQILDYNLERSMIEEVFQEEIDGSTLKEIYENQIRETISDLDYFTVEKRTQAQRLFRNMAGYKVFIPDAVLRYSESEIERTFAELIKGDYRLWKDILGEIERITGEKTTMWDLKKVLYEYSVNVPVFYEEKSDFWSRTTGEFKGFYVWGSMEDDDVELLEELGLDSIFGETESSLIV

cas3

Putative CRISPR-associated nuclease/helicase Cas3

746 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity.

In the central section; belongs to the CRISPR-associated helicase Cas3 family.

82.292 kDa

MKRLLAKSTATPDRPEGEATLLGHTALVLSAARRLLEHRGRASLLAAGLDPALEPRLRRIVLLAAALHDLGKCSEHFQSMLRRQREAPQLVRHEALSLWLCWPGQPLSAWLQRDVSELDLCLALVCVAAHHRKFQTEAFAPDGTGAGLSLELRVQHEDFARTLGRIAEELALSAPPLFTAPIVLRATRKEHPRDQLQSWQDDFERTVPAGSVDARLLAVCKALVLAADVAGSALPRSGEKQDWVDRQLTAPHPAEALRAVVERRLAGHTPRPFQEEVARSAAPLTLVRAGCGSGKTAAAYLWAARQHPGRPLWLTYPTMGTATEGFRDYLHGADVEARLQHSRAEVDFDIFGLRDGAAPGTSSRDQDRLDALRSWGADAMSCTADTVLGLVQSQRQGLYAWPGLCAACVVFDEVHAYDDRLFGCLLRFLEALPGIPALLMTASLPATRLDVLRGMCERVHGRSLAEVEGPEDLETLPRYQRLDVAEPWALVAECLRDHGKVLWVSNTVDRCMRTAEAGTSHGARALLYHSRFRYEDRVRRHGDVIEAFATEGRAAFASTTQVAEMSLDLSADLLVTDLAPIPALIQRLGRLNRRSTLERPAPVRPFVVLPFDGPPYAAPDLRDARAWMERLGTGPLSQRDLVDAWGPPGMMDAPRRQSSTWLDGRFDTWPAPCRDGSPSLTVLLEEDARAVLDGAVSAHRVTLPMNLPPESFKWRAWPRAGRLPYPIPPANALDYDGLRGARWRKP

cas3

Putative CRISPR-associated nuclease/helicase Cas3

728 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity (By similarity).

In the central section; belongs to the CRISPR-associated helicase Cas3 family.

86.467 kDa

MHDIGKITEGFQNNIKKGKRSNKHPHPLYALPIIKNIEFDYLFDIPIEVFAILSHHTQLYNNLYADYQQYKKGTFLIEEIKEFIKNSKEAYESLGFSKFFEFEDLKIEDIPKDAKPLELHRLRRKYWIEANNYIKSLSFDDKIKIKSIFSFMFSILQLCDDFASLNFSEYAKDREGIFDDVLENPEIYVPTLNIDNPISFILKDYEPYKFQKELYNSKNKFVMLFAPCGRGKTEGALLWALNALKNFKRNKIILAMPTQVTSNAMYDRLVKIFGEENVGLFHGKSFIKLRDSKEIEDEDDLEEIRDENFKGNVFFKPITITTIDHVIYSFVHGFSQADFALGNIQNSVIIFDEVHYYEKYTLEHLLTLFDILRRMDIPHLLMSGTLPNFLMNNLEGYELVVDEEGLNYKPFKLKCSENHLIWKEDDEWKVNENIINEIIENYKKGLSQAIILNTVERAREFYKAVRDKVPAILYHSQFAYKDRVKKEDEIFTLEEMRKSQNKPYVIVATQVIEISLDMSVDVMYSELSPPDALGQRAGRLHRKGKDWRENGKEYKLKIFLPYKHLPYSKELIEKTINHIKFYEKPLDYRDIKDFVDNVYKDYNLSIPSDLKLFFDEAILFGRHWTDISTIDEEGRFFKVRDDKFMKIEVVPQVYFDELGENSLRAEYMAKIPAYLILNEMKNEEGLIHFYPYEKRVGRKTRRYLICSFKYTYEIGFDYKEEEEFEDIL

cas3

CRISPR-associated helicase Cas3

614 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Probably unwinds dsDNA templates, thus providing substrates for CRISPR-associated endonuclease Cas3-HD. Unwinding is strongly increased in the presence of ATP, implying the helicase uses ATP to unwind substrate.

Belongs to the CRISPR-associated helicase Cas3 family.

71.568 kDa

MDIINFFKQITDHEPYDYQIRAWEKINKIMELGGRVVIEIPTAGGKTEAAIIPYLYQFISNDWKVPRLIYVLPTRSLVEKQVERIRNYIKKILEIKGYSKDKVEELAKKIVQVEYGLEETHAFLGFVVLTTWDTFLYGLAAHRTVGDRFTFPCGAIAQSLVVFDEIQMYQDESLYMPRLIGLVVKRLVEANVPLVFMTATLPTELKKILGIHDEEPITVNPEDTKKPERGEVVVEFKEKLSDEELSNEIKKAINEGKKVLIIKNTVNSAIEVYEKVKQLGNSLLLHSRFTVEDRAEKEKDIDKAEIIVATQVVEAGLDLTNVGLVITDLAPLDALIQRIGRCARRKGERGKVIVVLPNFNEKINEDHREKVILGFKNIPFENAYVTLVNNKDYGRVIELTIETIEEKKGKSKIVPKKFYIGDLGNKTIRKLIENNKILKKKDLYFIPYSTQPYDPLIMIKSFDEVESVSEYLYDIIKAREALDRVYKEYYENNIVPKDYYSAYIYFRELKLFSTPPEYELKARPEMFAILYPLENAEEKVKNKEIIEFNPKYVIRVSYNWLKNKWEKFDKKFELIKEYDEKGWICSLKKAGKLQPYKIYIIDDKYYSKETGIII

cas3

CRISPR-associated helicase Cas3

566 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Probably unwinds dsDNA templates, thus providing substrates for CRISPR-associated endonuclease Cas3-HD. Unwinding is strongly increased in the presence of ATP, implying the helicase uses ATP to unwind substrate (By similarity).

Belongs to the CRISPR-associated helicase Cas3 family.

63.347 kDa

MVGRRLDTLVRELSLAWCRSKGEPSCAVREDLLAEQAKAAEVIERSDGVILLKAPTGFGKTEIWTAPFFAQWLRGEWFAPRMYVVEPMHALLRQMKRRMEVYAQAVQGLGLPRLNVAEDHGEVAKPLFLYGGHIVLTTVDSLAYGYLARRVQRWREEGVERGRYSMPAGLLASAYIVLDEAHLIQDEAYLGPRVLGKIVCDLASAGAKVVISTATVPETFLKHIPCLGGRLTLGSGTVRRNVKVERRKGVLKAEEIECGKPTIVIVNTIERARRIYKQVRCGKKAVVHSLMRREDKERQLSKVLADGKVAEDAVLIGTQALEVGLDFSNLRALYTETAPVDALIQRIGRVGRDGDKAEAYIYEAEGDAPYPQTLMRATREALEKELLGGAALTSWEDAQRAVDKVYNEKAVEELMTRGLAWYGQALGYLQELSLFSYPPRGEVRIRPSNYITLVIADVKQDGDKGRYITEDDVERGAMKMSYTSRDDPRINALLQKVSTAYTVRGVATAKDETRYYLSELRRSWDGVEVVVVDRRDVEELYDEAGLDVAQLSGGGQKRKGRGRRRR

cas3

CRISPR-associated endonuclease Cas3-HD

244 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Acts as a ssDNA and ssRNA nuclease, with both endo- and 3' to 5' exonuclease activities, acting on substrates with free single-stranded 3' ends. Double-stranded nucleic acids are not substrates. Activity is higher for DNA than RNA. Templates include R-loops (a bubble-like structure formed when ssRNA replaces one strand in a dsDNA, such as crRNA is thought to form with CRISPR target DNA), circular ssDNA, 2',3'-cAMP and 2',3'-cGMP. Probably generates 3'-phosphate and 5'-hydroxyl ends. In the presence of the Cas3 helicase and ATP dsDNA templates are also degraded.

Belongs to the CRISPR-associated nuclease Cas3-HD family.

28.406 kDa

MILGEIMEVLAFKNQSLIDHVNDMVKYWERIKYRYLKTIKRALEALNIKLDIEKVDEFMKILIKLHDIGKASKIYQRAIINDQEKLMGFRHELVSAYYTYHILLKKFGDKNLAFIGALTVMLHHEPIIMGQIRNLKKKELTAEVVLDKLKKFDGMIEDFEDLIKKLIGYSIGDIIKNDSNKDDIIRFVIEMSVRARHTPNSEKLRFIVGTLLLPLVMCDYKGAESREGKAPKFAEVLEVESYVI

cas3

CRISPR-associated endonuclease Cas3-HD

239 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Acts as a dsDNA and dsRNA endonuclease (in a fusion with Alicyclus caldarius esterase to increase solubility).

Belongs to the CRISPR-associated nuclease Cas3-HD family.

27.515 kDa

MIKPCAYEKQGLIDHAIGSYRVLDGKISESYYKIISRRLERYGIVLDLNGVKEMVKDVVVLHDMGKAGEYYQNQFDDNCNPLKSNFSFIYHELGSALFFYYDYEPIDVEKAEEVKSLLTLAVLNHLNAIRVISDYLVNKFPDNFDERMIKLNKYGSIMLQNLRGVISKSLKVRDYTFDDYHDMLYAFSKKSDKYLKLYNLFLAPIMLGDNLDSSLVRNNGSKTGFVRILEGELNGGSTL

cas3

CRISPR-associated endonuclease Cas3-HD

237 amino acids

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Acts as a ssDNA and ssRNA nuclease, probably with both exo- and endonuclease activities. Activity is higher for DNA than RNA. Templates include 2',3'-cAMP and 2',3'-cGMP.

Belongs to the CRISPR-associated nuclease Cas3-HD family.

27.077 kDa

MSCKAFQGQTLREHIEAMLAAWEIVKNKYIPSIIRVMKTVGVKFTEEDADKFMKTLIILHDVGKCSEVYQKHLSNNEPLRGFRHELVSAYYAYNILKDMFKDETIAFIGALVVMMHHEPILMGQIRSLDKEELTPEVVLDKLRTFNGVMEGTESFIKSMIKEKLGVIPKVPSPTQEDVLREVIRLSVLARHRPDSGKLRMVVGALLIPLVLCDYKGAKEREGESPKFAEVLRVEMMK