bzaB

5-hydroxybenzimidazole synthase BzaB

427 amino acids

Together with BzaA, catalyzes the conversion of aminoimidazole ribotide (AIR) to 5-hydroxybenzimidazole (5-HBI) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction. Is thus involved in the anaerobic biosynthesis of dimethylbenzimidazole (DMB), the lower axial ligand of vitamin B12 (cobalamin). Requires BzaA for catalytic activity, as BzaB alone displays no activity.

Belongs to the ThiC family. 5-hydroxybenzimidazole synthase subfamily.

46.04 kDa

MTQMLEARKGHITEEMEKVALIEGVTPEFVREGVAKGHIVIPKNKFRSRDKICGIGGGLDVKVNGLMGTSSDRNDMEMEAKKLRILEECGANAFMDLSTGDDIDAMRKQSLTISNIAAGCVPVYQASVEAIEKHGSMVGMTEDELFDTVEKQCQEGMDFMAIHSALNWSVLNALKKSGRVTDVVSRGGSFLTAWMFHNKKENPLYEHFDRLLEILKATDTVLSIGDAIRPGANADSLDSAQVQGLIVAGELTKRALEAGVQVMIEGPGHVPLNQIATTMQLQKQLCYGVPYYILGFLATDVAPGYDNITGAIGGAFAGMHGADFLCYLTPAEHLGLPNEDDVRMGVRTTKIAADAANVLKRGGNAWNRSLAMSKARVARDEKVQVANALDPEYLESKLKAEPESHGCAACGKSKCPADVAAEFFGIA

bzaB

5-hydroxybenzimidazole synthase BzaB

426 amino acids

Together with BzaA, probably catalyzes the conversion of aminoimidazole ribotide (AIR) to 5-hydroxybenzimidazole (5-HBI) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction. Is thus involved in the anaerobic biosynthesis of the benzimidazole lower axial ligand of the cobamide produced by M.thermoacetica (PubMed:26246619). Requires BzaA for catalytic activity, as BzaB alone displays no activity (By similarity).

Belongs to the ThiC family. 5-hydroxybenzimidazole synthase subfamily.

45.58 kDa

MSQVLDARAGKITPEMEKVAADEKVDVEFVRAGVAEGTIVIPRNTNRKVLKPCGIGRGLRIKVNALIGTSSDRDDRQMEMRKIAAAEAAGCDSFMDLSTGGDIDEMRRLTLAHARVPVGSVPIYQAAIEAIEKRGSIVAMTPDDMFAAVEKQARDGIDFMAIHSALNFEILERLQASGRVTDIVSRGGAFLTGWMLHNQKENPLYEQFDRLLEILLKYDVTLSLGDAIRPGSTADSLDGAQLQGMIVAGELVRRAREAGVQVMVEGPGHVPLNHVETTMKLQKSLCGGAPYFILGTLATDVAPGYDHITAAIGGALAGTVGADFICYVTPAEHLGLPTEQDVKEGVIAARIAAHAADLARGNRQAWERDLQMARARVALDVEKQISLAIDQEKARSLLDGTGEDGVCAACGTNCAALVAARYFGMN