Function
Serine/threonine-protein kinase essential for spindle-assembly checkpoint signaling. Plays a key role in the recruitment of the checkpoint proteins bub-3, mdf-1 and mdf-2 to unattached kinetochores (PubMed:18936247, PubMed:19109417, PubMed:24567362, PubMed:25987605). mdf-1 recruitment is independent of bub-1 kinase activity (PubMed:24567362). Has a role in the correct kinetochore localization of the spindly-like protein spdl-1 (PubMed:18765790). In addition, during meiotic anaphase I, controls the recruitment of hcp-1/2 and klp-19 to the ring-shaped domain formed between chromosomes (PubMed:20729837). Involved in chromosome alignment, chromosome homolog segregation and spindle assembly (PubMed:20729837, PubMed:25987605). In association with bub-3 at the kinetochore region of chromosomes, promotes the onset on anaphase independently from spindle checkpoint signaling and promotes the formation of stable end-on bipolar attachments of chromosomes (PubMed:25987605). Plays a role in nuclear envelope breakdown (PubMed:19109417). Required maternally during embryogenesis and in the zygote for the postembryonic development of several tissues including ventral cord neurons, gonad, intestine and seam cells (PubMed:19526056).
Sequence
MSHIRVAFAPLDTNPSTCGLETFATQIETLSQMMTAEEMKAELYDCVRRILREQKFDQIRENEAMLRLYKVMGRSSTNLKGRGIYEQLFKKDHFTGSLKFYLQWAEECGKDQMLEEFKDVLKLARDRLSERIEMTAIESGFRDLVDEYFNGESGDMFTRPDETMDLFRFNAGSKKKRRSSVCFLQHNVPINNSGKAAFGPKTKTDLRQACIDRPNYHGISIEEFRFAKWKDTFGEDVDDDYRKRKDSGVVFVKHQVIDTDRQAREEVENRFNANLNPRRRHLSPVSEKTVDDEEEKRSRIYSPLVATKDAHRPALRSKIENPPATVTLSSDTKSASEKDVSDSDDADDDERLKIMTAGRKDGNPPDRSTSISSNYSTASARTSKSGAGLDLMAENKCLEAHAMFSDTVHLASEKTMVLGDDSVFVPERSLATTQIVTDFSVLCDPDPTMTITQERPKKVSNGLNVVYDEAAEPEESQKVEESEVQPEIVLVSPVTQTSPATMFNDIYDDEIEFGFFKPSRGNFVTSTPAQGVHLVNIDEYFGNKEEESTHEQEAPVFVAPTSSTFSKLTRRKSLAANQAVQPSVTESSKPERSDPKDSSIDCLTANLGRRLSIGADEIPNLTENNESEITGCKIRRRSEIIKQGDINPWDETLRKKLMCLVRPPQNMHEFQERAPKIQALRDCEVSGEKLHIQTLIGQGGYAKVYRAVTDDQRTVAVKYEVPSCSWEVYICDQMRNRLKDRGLELMAKCCIMEVMDAYVYSTASLLVNQYHEYGTLLEYANNMKDPNWHITCFLITQMARVVKEVHASKIIHGDIKPDNFMITRKIDDKWGKDALMSNDSFVIKIIDWGRAIDMMPLKNQRFKGRAGTEAFDCPEMVDGRSWTYQADYFGFAATMAVVVAGKYAQLTGASVGDYSLNVDIKRRNILRDACYDVINRFLNIPSCDSLPDWNILIKSFSEIWNEKFEASGWRQAVSKFNEACDLAANQK