btrH

Gene

btrH

Protein

Ribostamycin:4-(gamma-L-glutamylamino)-(S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] 4-(gamma-L-glutamylamino)-(S)-2-hydroxybutanoate transferase

Organism

Bacillus circulans

Length

302 amino acids

Function

Aminoglycoside acyltransferase that attaches the (S)-4-amino-2-hydroxybutyrate (AHBA) side chain from the acyl carrier protein BtrI to the aminoglycoside ribostamycin in the biosynthetic pathway of butirosin. The AHBA side chain protects the antibiotic from several common resistance mechanisms.

Mass

35.885 kDa

Sequence

MCLTRYDEKFFDCRKSQIIAYLDSQQVPVIPLFYNSYQSTAEIYRQIFIENKSKWKYSEPSFSDDDLLRKGIRPVRASFPDFSQASDCLKDLLARHKLVFVWGDEYCLPYRKEAFQAIHSTHSLVVTGYDGENKAYYVEDWDGLYGYLPAVHLEAAFDSLSRQMRTLLVLELNDEEMRENKQEDTDLFRKWLQAFEDDYIFYDRVLLDMRDYEENRLISMDHGLRLIAASRHVFSKFLHYIDDAPEEVGLLIRNHQLANHIAAIVRRYIIAKQIDWDGAACKIRQLREQEDDFMRKLKSRYG

Protein

Ribostamycin:4-(gamma-L-glutamylamino)-(S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] 4-(gamma-L-glutamylamino)-(S)-2-hydroxybutanoate transferase: btrH