Protein
Modification methylase BstVI
Organism
Geobacillus stearothermophilus
Function
This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BstVI endonuclease.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family.
Sequence
MVTQHSYRMHGAVYTKPIIVDLILDLTGYTSDKNLENFKLLDPSFGDGVFLEAAVHRLMDSLIRRGYRPNELIDHLGNCIRGIELRLEAYQAGRHRLQKVLEGYGFSKPEINWLINQWIIQADFLLWQEDTTEAIKFDFVVGNPPYVRQELIQDELIKKYRKRYTTIYDRADLYVPFIQHSLELLSEQGTLGIICSDRFTKNRYGKKLRKFITDNYKVRYIVDLHKTSPFENEVTAYPAIYVIKTKNYDKSVVRAVYTEVITSKVCQDAKDFLLSNQKPDQSSKEMKTYVFSEWFAGDEPWIIQSQECREILRRLENRFPLIEDDVHSCKIRIGVATGADKVYIVDPQQVDIEPEVLLPLVTTADISSGRIIWSGKHVINPFNSDGGLINLDDFPRLKTYFQQHEEIIKNRNVAKKNPSQWFRTIDRIYPEIVHQPKLLIPDMKNTNHIVKDDGAFYPHHNLYYILPGNWNIDILRAILLSSVVKFFIWSYATKMRGDTLRYQAQYLRKIRLPDPKSLTNDQKERLMDERVIQSQEYLDSIVAEIYQLSKTEIEIIKDALE