Protein
Modification methylase BsoBI
Organism
Geobacillus stearothermophilus
Function
This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsoBI endonuclease.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.
Sequence
METVQMSLDLQPEDTKANIECSSPDYEIKCFSHNQFAPQIERLKIEKKYIHIVEETETFNRKLVSYQANKNQSIHNWIRYKEGFSSELVQNLIEEFGLSKGDTILDPFLGSGTTSLTAKMLGINSIGIDILPISHIAFEPKSFIFEYNLEELDRAYKEIYEISPTKIEQKFNHLSITEGAFPEETENDLLFFTHWDNNSQYSYQTKTLIKLILVSILEEISYTRKDGQYLRWDYRSQKVIETNKKRLEQGKEPIKTILDKGELPTVKESLLNTLLTIKEDIKEIQQKCLPNESVHELIKDSALNALPKINDNTFDAVITSPPYCNRYDYTRTYALELAYLGVDEKKIRELRQAQLSCTVENKSKLKQLKDYYHSLFLESRYAEIERLVTGNEVLNEINYALRKRWENGEVNNKGILSMVDGYFTELTFIFYELFRTCKPGAKVAFVNDNVRYAGEIIPVDFLSTKIAEDIGFKPIKIYTLKQRKGNSSQQMGKYGRVALRKSITIWEKP