brr2

Pre-mRNA-splicing factor brr2

2176 amino acids

Involved in pre-mRNA splicing. May be involved in endoplasmic reticulum-associated protein degradation (ERAD) and required for growth at low and high temperatures (By similarity). Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region.

248.809 kDa

MSSAHPKGDSKEPPKHGNSKEKPNYGQSQYSYSAMSNLVTQADRRFVSRRDAEPTGEPESLVNRVSIADMGSRARIEKPSTLPLELTQEVQEVRLPRKDAESLEIGIRQPEREKRSSAILKYFDSFEILKYNPLTDETREVYDYILSFIQQYLGDQSPEILRSAADLIIELLKDSSLDEQGRKKQIEEVLSTELPQDRFSQLVNLGNRLTDYTVEQEEELNEEGVNESGVPVLFNEADEEEEAVEAMEEDEVAEDEDVVLETSISQEEEKKNIENPDTEVTFISADTKKVTEIPTVHPREIDAFWLQREIAKYFADAVVCQEKTNQAFEALSADYDLGELENELMSIFDYEHFYLVQLLTKNRWTIVSCTMLKRAATDEERLGVEEQIRAAGRSWILEALRPGAITIPDDGLNELNNNVVEKAEPAPVSEIPLSKTLTSHKIVPKHQVDLENYVFTEGSRLMSNKAVKLPEGSFRRTGKGYEEIHVPAPNKAVLGADERLVKIKELPEWSHQAFLNTQSLNRIQSHLYPIAFGTDENILLCAPTGAGKTNVAMLCILNELQKHLREDLSFNLQNFKIVYIAPLKALVQEMVNNFSKRLTPYNIRVAELTGDSQLTKQQISETQIIVTTPEKWDIITRKANDLSYVNLVRLVIIDEVHLLHDERGPVLESIVARIFRHQEETLEQVRLVGLSATLPNYTDVASFLHVDPKKGLFYFDSTYRPCPLKQEFIGITEKTPFKRMQTTNEACYEKVMQHAGKNQVLIFVHSRKETAKTARFIRDKALEEETIGHLLRSDAASREILRAEADSTSDENLKDLLPYGFAIHHAGMRREDRQTSEDLFADGTIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGIWTELSPQDVLQMLGRAGRPQFDTYGEGIIITAHSELQYYLSLMNQQLPIESQFMRRLADCLNAEVSLGTVRSIEDGVDWLGYTYLYVRMLRSPALYSVGPEYDDDKYLVQKRADLLHSAAILLEKCKLLVYNRQSGTLTATELGKVAASYYVTHNSMAIYNRLLMQTTSFIELFRVFSFSDEFKHIPVREEEKVELAKLLERVPIPIRERLDEPAAKINALLQSYISRQRLDGFALVADMVYVTQSAGRIMRAIFEISLRRGWSSVATLSLDTCKMIEKRLWPTMSPLRQFPNCPSEVIRRVEKKEFPWQRYFDLDPAELGELVGVPKEGRRVYNMVQSFPRLSVEAHVQPITRSLVRVELVINSQFNWDDHLSGTSEAFWILVEDVDGDRLLHYEQFFLLKKYKDDEHIVNFTVPLLEPLPPCYFIKIVSDRWLHSITKVPLSFQRLIMPEKFPAPTPLLDLQNAPVSSLNNPSFISLYPNFKFFNKIQTQVFNSVYKTNDSVFIGAPNGSGKTVCAELALLHHWSQEDYGTAVYIAPIQEIVDRRYEEWYGKFSDLGDGKVLVKLTGERSQDLKLIQVADLIFCTPSQWDSLSKRWRSMRSIQKVDFYICDELQLLGGFYGPLYEIVISRIRYMAVQLEKNIRVVGLSVSVANARDLGEWLGTSPQCIFNFSPKDRPNPLTIHLQSFSITHFPSLMLAMSKPIYRSLKNFISQRKSTIVFTPDRKVAKQLAFDLVTFSMADEDEYLFSLMENEAFNKVEDAALQQSLKHGIAYISEITSSNDQNIVQYLYRHGLIKVLIASRDVIYSLKAKSNAVIVMGTQYYDGKEHRYIDYPISELLQMLGFTASIGSSELSQVILMTVTTKKEYYKKFLNEPLPMESHLQVWLHDAFVSEISTQTIESKQDAVDWLTWSYMYRRLVANPAYYGLQDITHESVSEFLSDLVETTMNDLSEARLITVDDEDDSCVALNLAMIASHYGITYITMQTFALSLSERTKMKGLLEIVTSAAEYEQLPIRKYEDIVLERIHSRLPVRLSNPNYEDPHTKSFILLAAHFSRFELPPGLVIDQKFILTRVHNLLGACVDTLSSEGHLIACIRPMEMSQMVTQALWDRDSPLKQIPYFDDALIERCNKEGVHDVFDIIDLDDEKRTELLHMDNAHLAKCAEFINKYPDIDIDFEIEDSEDVHANSPSVLIVQLTRELEEDEEVDTTVIAPYFPAQKTEHWWLVISDDKTLLAIKKITLGRSLTTKMEFVPPAMGTLKYKLSCFSDSYMGVDYEKEFECNVLEPLDTEMEDGE

BRR2

Pre-mRNA-splicing helicase BRR2

2163 amino acids

RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.

Belongs to the helicase family. SKI2 subfamily.

246.185 kDa

MTEHETKDKAKKIREIYRYDEMSNKVLKVDKRFMNTSQNPQRDAEISQPKSMSGRISAKDMGQGLCNNINKGLKENDVAVEKTGKSASLKKIQQHNTILNSSSDFRLHYYPKDPSNVETYEQILQWVTEVLGNDIPHDLIIGTADIFIRQLKENEENEDGNIEERKEKIQHELGINIDSLKFNELVKLMKNITDYETHPDNSNKQAVAILADDEKSDEEEVTEMSNNANVLGGEINDNEDDDEEYDYNDVEVNSKKKNKRALPNIENDIIKLSDSKTSNIESVPIYSIDEFFLQRKLRSELGYKDTSVIQDLSEKILNDIETLEHNPVALEQKLVDLLKFENISLAEFILKNRSTIFWGIRLAKSTENEIPNLIEKMVAKGLNDLVEQYKFRETTHSKRELDSGDDQPQSSEAKRTKFSNPAIPPVIDLEKIKFDESSKLMTVTKVSLPEGSFKRVKPQYDEIHIPAPSKPVIDYELKEITSLPDWCQEAFPSSETTSLNPIQSKVFHAAFEGDSNMLICAPTGSGKTNIALLTVLKALSHHYNPKTKKLNLSAFKIVYIAPLKALVQEQVREFQRRLAFLGIKVAELTGDSRLSRKQIDETQVLVSTPEKWDITTRNSNNLAIVELVRLLIIDEIHLLHDDRGPVLESIVARTFWASKYGQEYPRIIGLSATLPNYEDVGRFLRVPKEGLFYFDSSFRPCPLSQQFCGIKERNSLKKLKAMNDACYEKVLESINEGNQIIVFVHSRKETSRTATWLKNKFAEENITHKLTKNDAGSKQILKTEAANVLDPSLRKLIESGIGTHHAGLTRSDRSLSEDLFADGLLQVLVCTATLAWGVNLPAHTVIIKGTDVYSPEKGSWEQLSPQDVLQMLGRAGRPRYDTFGEGIIITDQSNVQYYLSVLNQQLPIESQFVSKLVDNLNAEVVAGNIKCRNDAVNWLAYTYLYVRMLASPMLYKVPDISSDGQLKKFRESLVHSALCILKEQELVLYDAENDVIEATDLGNIASSFYINHASMDVYNRELDEHTTQIDLFRIFSMSEEFKYVSVRYEEKRELKQLLEKAPIPIREDIDDPLAKVNVLLQSYFSQLKFEGFALNSDIVFIHQNAGRLLRAMFEICLKRGWGHPTRMLLNLCKSATTKMWPTNCPLRQFKTCPVEVIKRLEASTVPWGDYLQLETPAEVGRAIRSEKYGKQVYDLLKRFPKMSVTCNAQPITRSVMRFNIEIIADWIWDMNVHGSLEPFLLMLEDTDGDSILYYDVLFITPDIVGHEFTLSFTYELKQHNQNNLPPNFFLTLISENWWHSEFEIPVSFNGFKLPKKFPPPTPLLENISISTSELGNDDFSEVFEFKTFNKIQSQVFESLYNSNDSVFVGSGKGTGKTAMAELALLNHWRQNKGRAVYINPSGEKIDFLLSDWNKRFSHLAGGKIINKLGNDPSLNLKLLAKSHVLLATPVQFELLSRRWRQRKNIQSLELMIYDDAHEISQGVYGAVYETLISRMIFIATQLEKKIRFVCLSNCLANARDFGEWAGMTKSNIYNFSPSERIEPLEINIQSFKDVEHISFNFSMLQMAFEASAAAAGNRNSSSVFLPSRKDCMEVASAFMKFSKAIEWDMLNVEEEQIVPYIEKLTDGHLRAPLKHGVGILYKGMASNDERIVKRLYEYGAVSVLLISKDCSAFACKTDEVIILGTNLYDGAEHKYMPYTINELLEMVGLASGNDSMAGKVLILTSHNMKAYYKKFLIEPLPTESYLQYIIHDTLNNEIANSIIQSKQDCVDWFTYSYFYRRIHVNPSYYGVRDTSPHGISVFLSNLVETCLNDLVESSFIEIDDTEAEVTAEVNGGDDEATEIISTLSNGLIASHYGVSFFTIQSFVSSLSNTSTLKNMLYVLSTAVEFESVPLRKGDRALLVKLSKRLPLRFPEHTSSGSVSFKVFLLLQAYFSRLELPVDFQNDLKDILEKVVPLINVVVDILSANGYLNATTAMDLAQMLIQGVWDVDNPLRQIPHFNNKILEKCKEINVETVYDIMALEDEERDEILTLTDSQLAQVAAFVNNYPNVELTYSLNNSDSLISGVKQKITIQLTRDVEPENLQVTSEKYPFDKLESWWLVLGEVSKKELYAIKKVTLNKETQQYELEFDTPTSGKHNLTIWCVCDSYLDADKELSFEINVK