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arfA

Gene
arfA
Protein
Peptidoglycan-binding protein ArfA
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Length
326 amino acids
Function
May play a role in ammonia secretion that neutralizes the medium at pH 5.5, although it does not play a direct role in ammonia transport. Binds peptidoglycan. Expression depends on arfB and arfC (By similarity).
Similarity
Belongs to the outer membrane OOP (TC 1.B.6) superfamily. ArfA family.
Mass
33.574 kDa
Sequence
MASKAGLGQTPATTDARRTQKFYRGSPGRPWLIGAVVIPLLIAAIGYGAFERPQSVTGPTGVLPTLTPTSTRGASALSLSLLSISRSGNTVTLIGDFPDEAAKAALMTALNGLLAPGVNVIDQIHVDPVVRSLDFSSAEPVFTASVPIPDFGLKVERDTVTLTGTAPSSEHKDAVKRAATSTWPDMKIVNNIEVTGQAPPGPPASGPCADLQSAINAVTGGPIAFGNDGASLIPADYEILNRVADKLKACPDARVTINGYTDNTGSEGINIPLSAQRAKIVADYLVARGVAGDHIATVGLGSVNPIASNATPEGRAKNRRVEIVVN

Gene
arfA
Protein
Peptidoglycan-binding protein ArfA
Organism
Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Length
326 amino acids
Function
May play a role in ammonia secretion that neutralizes the medium at pH 5.5, although it does not play a direct role in ammonia transport. Binds peptidoglycan (By similarity). Expression depends on arfB and arfC.
Similarity
Belongs to the outer membrane OOP (TC 1.B.6) superfamily. ArfA family.
Mass
33.574 kDa
Sequence
MASKAGLGQTPATTDARRTQKFYRGSPGRPWLIGAVVIPLLIAAIGYGAFERPQSVTGPTGVLPTLTPTSTRGASALSLSLLSISRSGNTVTLIGDFPDEAAKAALMTALNGLLAPGVNVIDQIHVDPVVRSLDFSSAEPVFTASVPIPDFGLKVERDTVTLTGTAPSSEHKDAVKRAATSTWPDMKIVNNIEVTGQAPPGPPASGPCADLQSAINAVTGGPIAFGNDGASLIPADYEILNRVADKLKACPDARVTINGYTDNTGSEGINIPLSAQRAKIVADYLVARGVAGDHIATVGLGSVNPIASNATPEGRAKNRRVEIVVN

Gene
arfA
Protein
Peptidoglycan-binding protein ArfA
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Length
326 amino acids
Function
Probably plays a role in ammonia secretion that neutralizes the medium at pH 5.5, although it does not play a direct role in ammonia transport.
Similarity
Belongs to the outer membrane OOP (TC 1.B.6) superfamily. ArfA family.
Mass
33.574 kDa
Sequence
MASKAGLGQTPATTDARRTQKFYRGSPGRPWLIGAVVIPLLIAAIGYGAFERPQSVTGPTGVLPTLTPTSTRGASALSLSLLSISRSGNTVTLIGDFPDEAAKAALMTALNGLLAPGVNVIDQIHVDPVVRSLDFSSAEPVFTASVPIPDFGLKVERDTVTLTGTAPSSEHKDAVKRAATSTWPDMKIVNNIEVTGQAPPGPPASGPCADLQSAINAVTGGPIAFGNDGASLIPADYEILNRVADKLKACPDARVTINGYTDNTGSEGINIPLSAQRAKIVADYLVARGVAGDHIATVGLGSVNPIASNATPEGRAKNRRVEIVVN

Gene
arfA
Protein
Peptidoglycan-binding protein ArfA
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Length
326 amino acids
Function
Probably plays a role in ammonia secretion that neutralizes the medium at pH 5.5, although it does not play a direct role in ammonia transport. The OmpA-like domain (196-326) binds M.tuberculosis peptidoglycan. Overexpression in M.bovis or M.smegmatis gives channels with average conductance value of 1,600 +/- 100 pS, but this may not be physiologically relevant.
Similarity
Belongs to the outer membrane OOP (TC 1.B.6) superfamily. ArfA family.
Mass
33.574 kDa
Sequence
MASKAGLGQTPATTDARRTQKFYRGSPGRPWLIGAVVIPLLIAAIGYGAFERPQSVTGPTGVLPTLTPTSTRGASALSLSLLSISRSGNTVTLIGDFPDEAAKAALMTALNGLLAPGVNVIDQIHVDPVVRSLDFSSAEPVFTASVPIPDFGLKVERDTVTLTGTAPSSEHKDAVKRAATSTWPDMKIVNNIEVTGQAPPGPPASGPCADLQSAINAVTGGPIAFGNDGASLIPADYEILNRVADKLKACPDARVTINGYTDNTGSEGINIPLSAQRAKIVADYLVARGVAGDHIATVGLGSVNPIASNATPEGRAKNRRVEIVVN

Gene
arfA
Protein
ADP-ribosylation factor 1
Organism
Dictyostelium discoideum
Length
182 amino acids
Function
GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
Similarity
Belongs to the small GTPase superfamily. Arf family.
Mass
20.682 kDa
Sequence
MGLAFGKLFSRFFGKKDMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEFKNINFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEACDELTKMLNEDELRDAVLLVFCNKQDLPNAMSVAEVTDKLNLHSLRSRKWYIQSTCATSGDGLYEGLDWLSNTLTSSSK

Gene
arfA
Protein
Alternative ribosome-rescue factor A
Organism
Escherichia coli (strain K12)
Length
72 amino acids
Function
Rescues ribosomes stalled at the 3' end of non-stop mRNAs (PubMed:21062370, PubMed:21435036). This activity is crucial when the stalled ribosome cannot be rescued by the SsrA(tmRNA)-SmpB quality control system (PubMed:21062370, PubMed:21435036). Binds the 30S subunit, contacting 16S rRNA with the N-terminus near the decoding center and its C-terminus in the mRNA entry channel; contacts change in the presence of release factor 2 (RF2, also named PrfB) (PubMed:25355516, PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Requires RF2/PrfB to hydrolyze stalled peptidyl-tRNA on the ribosome; recruits and probably helps position RF2/PrfB correctly in the ribosomal A site so RF2's GGQ motif can hydrolyze the peptidyl-tRNA bond (PubMed:22857598, PubMed:22922063, PubMed:25355516, PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Does not release ribosomes with a programmed pause caused by regulatory chains such as SecM-mediated pausing (PubMed:22857598). Binds tRNA which may stimulate its ribosome rescue activity (PubMed:22857598).
Similarity
Belongs to the alternative ribosome-rescue factor A family.
Mass
8.171 kDa
Sequence
MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGKHGNRGNWEASGKKVNHFFTTGLLLSGAC

Gene
arfA
Protein
Alternative ribosome-rescue factor A
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Length
69 amino acids
Function
Rescues ribosomes stalled at the 3' end of non-stop mRNAs. Recruits release factor 2 (RF2) to the stalled ribosome, helping position it correctly in the ribosomal A site so its GGQ motif can hydrolyze the peptidyl-tRNA bond (By similarity).
Similarity
Belongs to the alternative ribosome-rescue factor A family.
Mass
8.154 kDa
Sequence
MRKKQKSAVENETVYLHTRGVIKDNAVMALLGDKLFRQRVEKKRKGKGSYQRKAKHPGKIFEKPDYKFF