Protein
Nonribosomal peptide synthetase anaPS
Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)
Function
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of the prenylated pyrroloindoline diketopiperazine acetylaszonalenin (PubMed:19001367). The first step in the pathway is the formation of (R)-benzodiazepinedione by condensation of tryptophan and anthranilic acid catalyzed by the non-ribosomal peptide synthetase anaPS (PubMed:19001367, PubMed:20225828). The prenyltransferase anaPT then converts (R)-benzodiazepinedione to aszonalenin in the presence of dimethylallyl diphosphate (DMAPP) via C3-prenylation (PubMed:19001367). The last step in the biosynthesis of acetylaszonalenin via acetylation of aszonalenin at position N1 catalyzed by anaAT (PubMed:19001367).
Similarity
Belongs to the NRP synthase family.
Sequence
MTITTMTPQNGTRRQVDGGPICFFPTMTRLDMQSITFDNHVVQKLTSFPNASDAGVHLLLAGLWALTLRQYAEVDTARFEVSTSILASGKGSGATKHVFSIALSPSDPVSTLFDVRNWDIRFVDQKHSDSFNTGVFVVENQNGRCLLDVEYHDINLLLQSNRTSAELSLVYRSSAIAGTYARHLADGIAQAIISISENPNQSIGAVDFCCSLQKAQVVTWQNAKIIQPDRSFLFEYISRNATVHGDTLAIDSWDGQFTYAELDGLSTVMATRFQERGIGPGDLVPMCFGKTRWAIAAMLAINKTGAGFVPLDPAYPQSRLETIIQKTQARVALASPTTESILRPLGLPLLVISDSILGCCLPHSKRYTAPNSGVAPAYCFFTSGSTGDPKGCEVSHLAFASIATHARSLCLSQQSRSLQFASFCFGASLLEIWCTLIVGGTLCIPSDHDRLNSLGEFMAKMRINWAFITPTVLASISPDNFNNLHLFIAGEPIGERDIRTWAPRARLFQAYGLTEWAGVFAVSRQIRTPEDRKSIGSPVNARAWIVDPLDHQKLAPIGAVGELVIEGPSLAQGYRGDPQRTAAVFLQRPPWLTLPALSKDGSSSRVYKTGDLVRYAEDGSLVYVRRKDNQVKIHGQRLEIGEVEYHVRQLFPQAKMVIVMVHEPSDAASHQRNLVALTLHPPNNGHTGFSHGKLEFMEVDQEYQSKVEHVRNGLRSRLPAFMIPQLFLPLSQIPTTITGKADRRSLCRDVNKLSYAQLHGLTTQMVSTRAAQGKGEEAIHAAVCDVLGLAPEHLGMNDNFFHLGGNSASAMKLTMSARRRGLRFTIRDVFNHPVLAELASAANLSNGCERPVVQTMELLEPESVSELKQLAVSQCRIDEDIIEDIYPSTALQEGLVAITARDPSLCKARVICKLRSNVRIDALKAAWECVVQLNDILRTRFILSASHGTFQVVCKEPFSWARAQNLEDCIQQSDALVHRVGDDLVHAYIIPDEKDRDSASTFVFVAHHALCDQWSIRLLLDQLTAAYGHSKLPSNRFSAFIRYLTKTRSHFKNYWINQFQGLEAVAFPPLPSPSYTPVASEKFDFVMKLLGNTTKQITTATYIKLAWAVVISCNTGSNDTVFGVTVNGRGAPIDGVGELTGPTIATIPQRIKLLPDQSATSALAEIQSHSLEVIPYEQAGLQNIQKYSPEARSACMFQSQLIIQPCPPSPPDLFEACDFSATQTGGFSAYGLSLECQMTYDDRHCEVTATFDPGMISRERVQRLLQHLELVLQDVMADPSRKVGDLPRMSRQDWDQIQRWSGTLPPVSRQCVHDAVDERYLEYPNACAVSAPDGDLSYAELIHSANAVAAELLAHGVEPGKYIPVLFEKCKWSPVAMLGVLKAGAAFVLLDPSYPPQRLHAICGGLKSQIILCSKGLSARAASLGPTAIAVHENATFLVDIPNATLPVVSPEDPAYVVFTSGSTGTPKGAIIDHQSYCSSALAHNRAHFLGRNSRVLQYASYAFDVSIMETLSTLMAGGCVCILSDLERHDHFADSVQRLAVTHAFLTPSTARLLMQRELPSLCVLVMGGEVMSLADRSYWMKRVRLMNEYGIAECSVASTIREVSDVEQRDIGFPMGVLAWVVDQNDHEKLVAIGAIGELLLEGPSVGRGYLDNPEATRRAFIEQPGWLRAVRGGKTSRVYKTGDLVQYNEDGSLSFIGRKDSQIKIRGQRFELEEVEQHLRRIDEIKEVTAVAVAPSDRQKQAYLVAFIVPRTRESFCVHSAKALVTHPTEEFRHLAAAIQSKLHSILPAHMVPSIYLPVNQMPKTSSDKVDRCRLKEEVGKWSWSDLQAYSVSSTSRRAPSNSVEQDLQRVWAQILGIRLDSIGVEDSFFHLGGDSIIAMQVVAEARSRGLDHSVQDINQLKSIKAIANKIGVVSTIAQPVVQDQVTDELFGLTPIQEFFFEKYPEGTCRFNQNILVHFQKPVADIDVERAANKLVQNHAILRARYARQKDGSWKQFFTGYTEQCFRFSMHKVNSVQEMRHIIGQSQTSLDPEHGPVFTVDLFDHNGQQSLFMIGHHLVLDLVSWRIILADMEAMILDPQHQPHLTMSFQTWARLQAEYGTRHLEPPPVQQLCSIDEPSMRKFWGAENNANTGGDSKTRLIRVNEQLTNKLFGPSSQALDVEPVELLHAAILFSFVNTFPQRPAPCIFGEAHGRETWDSSIDVTRTIGWFTTLWPVVAQVNPSDSLETVVRTVRQARRAMDMHGWKHFTSIYHNTQQTKCSAGTHLMEITFNYAGKFQQVEQDGALFRMEPMAKQNLFDGAAELGRWAMLEINSVILNGMLEFHVTYNRGTDEASVLTPWMDNLVKCLEDLASGFA