aglB

Dolichyl-monophosphooligosaccharide--protein glycotransferase AglB

1054 amino acids

Oligosaccharyl transferase (OST) that catalyzes the initial transfer of a defined glycan (a hexose-linked tetrasaccharide composed of a hexose, 2 hexuronic acids and a methyl ester of hexuronic acid in H.volcanii) from the lipid carrier dolichol-monophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (Probable). Involved in the assembly of an N-linked pentasaccharide that decorates the S-layer glycoprotein and flagellins (PubMed:17996897, PubMed:21091511) (Probable).

Belongs to the STT3 family.

113.675 kDa

MSDEQTKYSPSIAELARDWYHIPVLSTIILVMLWIRLRSYDAFIREGTVFFSGNDAWYHLRQVEYTVRNWPATMPFDPWTEFPFGRTAGQFGTIYDQLVATAALVVGLGSPSSDLVAKSLLVAPAVFGALTVIPTYLIGKRLGGRLGGLFGAVILMLLPGTFLQRGLVGFADHNIVEPFFMGFAVLAIMIALTVADREKPVWELVAARDLDALREPLKWSVLAGVATAIYMWSWPPGILLVGIFGLFLVLKMASDYVRGRSPEHTAFVGAISMTVTGLLMFIPIEEPGFGVTDFGFLQPLFSLGVALGAVFLAALARWWESNDVDERYYPAVVGGTMLVGIVLFSLVLPSVFDSIARNFLRTVGFSAGAATRTISEAQPFLAANVLQSNGQTAVGRIMSEYGFTFFTGALAAVWLVAKPLVKGGNSRKIGYAVGSLALIGVLFLIPALPAGIGSALGVEPSLVSLTIVTALIVGAVMQADYESERLFVLVWAAIITSAAFTQVRFNYYLAVVVAVMNAYLLREALGIDFVGLANVERFDDISYGQVAAVVIAVLLILTPVLIIPIQLGNGGVSQTAMQASQTGPGTVTQWDGSLTWMQNNTPAEGEFGGESNRMEYYGTYEYTDDFDYPDGAYGVMSWWDYGHWITVLGERIPNANPFQGGATEAANYLLAEDEQQAESVLTSMGDDGEGDQTRYVMVDWQMASTDAKFSAPTVFYDESNISRSDFYNPMFRLQEQGEQTTVAAASSLKDQRYYESLMVRLYAYHGSAREASPIVVDWEERTSADGSTTFRVTPSDGQAVRTFDNMSAAEEYVANDPTSQIGGIGTFPEERVSALEHYRLVKSSNSSALRSGSYQRSLISEGNTYGLQPQALVPNNPAWVKTFERVPGATVDGSGAPANTTVTARVQMRDLTTGTNFTYTQQAQTDADGEFTMTLPYSTTGYDEYGPDNGYTNVSVRAAGGYAFTGPTSVTGNSTIVSYQAENVAVDEGLVNGAEDGTVQVTLERNEQELDLPGDSSSEDSSSEDGTSDGSQTNESASTSTSASVDASAVSAAA

aglB

Dolichyl-phosphooligosaccharide-protein glycotransferase

933 amino acids

Oligosaccharyl transferase (OST) that catalyzes the initial transfer of a defined glycan (ManNAcGlc-2,3-diNAcAGlcNAc in Methanococci) from the lipid carrier dolichol-monophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. Involved in the assembly of an N-linked disaccharide that decorates the S-layer glycoprotein and flagellins.

Belongs to the STT3 family.

103.964 kDa

MYIKVKLMSNALEKINNFFKEKSWIKVFLIILMLMFVSFQLRAQTADMKFAQDNEFLKDMFSDEHGRMYLLALDPYYYLRLSENLYNNGHCGDTIKVVDGKETPYDLYQYAPPGHPLPWEPPVICLATLAIYYIWHSIDLTVTIMNAAFWVPAVLGMLLGIPIYFVVRRVTNSNIGGIAGAIALISAPGLLYKTCAGFADTPIFEVLPILFIVWFILESIHSQEKTALFKKDLKNPISLFVIAALIIELIIGAYLNIASGESVVIASILFYTVSLAFILAGLIIAGIKKLKGNELEFELFALLAVILTAVSPKMWGAWWYGFDVITAFLVIYIIALALLKSQVKIKEFINIGNLKNIVYLSIFYIFGSFVLLVAIYGMGIAISPITSPLGYNQILSTYTQTTGWPNVYTTVAELAKPSSWSEIFTNAIGSDTIAIVGILGILLSFLSLRYEKVKLDIKYSILLAIWLAVTLYAATKGIRFAALATPPLAIGLGIFVGQLERFLKMKSDIAIFGIGIPAGIFGLLILSKYSAKISQILLPTTYVPIIAYGFLIVLALLAIYKISDIISTLNDKKETIIKVSTLLLCIGVVIPPLSAVVPFSVAPTFNNGWKEGLDWIKANTPNNSVITCWWDNGHIYTYEARRMVTFDGGSQNSPRAYWVGRAFATSNENLSIGIIRMLATSGDEAFKKGSVLMNFTHNNVSKTVKILNEILPVDRSKAYDILTKKYGLSDKKAKLVLNATHPEHPNPDYLITYNRMTDIAPVWSMFGFWNFSLPPNTPNDKREKGAFFKGTAYYLGNGTILANVNVYTYSYVTLINSTNISTAIVQKINGQAKIIGTFKIHKLYIKTPLGVKELVLNKDGQLSEFIRIEADGRGYAWLATRNLEDSIYAKLHFLDGYGLKHIKLVKATIDPTDFGIQPGFKIYKVDYGTDYLK

aglB

Dolichyl-phosphooligosaccharide-protein glycotransferase

917 amino acids

Oligosaccharyl transferase (OST) that catalyzes the initial transfer of a defined glycan (ManNAcGlc-2,3-diNAcAGlcNAc in M.voltae) from the lipid carrier dolichol-monophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:24212570) (Probable). Involved in the assembly of an N-linked disaccharide that decorates the S-layer glycoprotein and flagellins (PubMed:16824110, PubMed:23624439).

Belongs to the STT3 family.

102.424 kDa

MTENNEKVKNSDSANNQSSKNSKFNFNFEDKKVKCAKTILIIIFLAFLSFQMRAQTADMGFTTNEQYLDVFSDDNGRMYLTALDPYYYLRMSENYLENGHTGDTLKNIDGQQVPWDSYKYGPTGARATFNLLSVVTVWVYQVWHAMDSTVTLMNAAFWVPAILSMFLITPIFFTVRRITSSDIGGAVAAILASLSPSIFVKTVAGFSDTPILEILPLLFIVWFIIEAIHYSKEKNYKSLIYGLLATLMLALYPFMWSAWWYGYYIVIAFLVIYAIYKGISYNSIAKYTKSKNNNHKDKIESEKLEMLNILKISGLFIIGGAVLITALYGVSTTMNALQAPLNYLGLDEVSSQTGWPNVLTTVSELDTASLDEIISSSLGSIHLFAIGLIGIFLSLFRKVLTPVKQISNGLAEKLDIKYALLLIIWFAVTFLAASKGVRFVALMVPPLSIGVGIFVGFIEQFIKNNLDKKYEYVAYPTIAIIVLYALFTIYRADSADLVRMLLPSNYVPIAEGIMLASLAVLIIYKVAELIAESNKKLVMNKIFMILLAIGLITPTIATIVPFYSVPTYNDGWGESLEWINTQTPNNSVVTCWWDNGHIYTWKTDRMVTFDGSSQNTPRAYWVGRAFSTSNESLANGIFRMLASSGDKAYTTDSVLIKKTGSIKNTVDVLNEILPLTKSDAQKALKNSSYKFTDTEVSEILDATHPKVTNPDYLITYNRMTSIASVWSYFGNWDFNLPAGTSRSEREAGSFQGLQTYATNINDTLIVRSLIQQTAEYNIYTLIEVRNETLTGAMMAVTNDGQMQTQQLNMHKVKLMVNENGKSKMYNSLADPDGQLSLLIKVDKNSIIGTDGSNNPVYSSSSWMATANLEDSVYSKLHFFDGEGLDTIKLEKESLDPTANGVQPGFKVFSVDYGNYSK

aglB

Cyclomaltodextrinase

473 amino acids

Is able to hydrolyze various linear maltooligosaccharides (maltotriose to maltoheptaose) and cyclomaltodextrins (CDs), to mainly glucose and maltose, by liberating glucose from the reducing end of the molecules. Shows a very weak activity on starch. Can neither hydrolyze maltose nor degrade pullulan, but rapidly hydrolyzes acarbose, a strong amylase and glucosidase inhibitor, to acarviosine and glucose.

Belongs to the glycosyl hydrolase 13 family.

55.181 kDa

MMYPMPSWVYDSVVYQIFPDRFFIGKGKTVEDKKDLYLKRGGVIEKWGVPPRKLPGAQHVKIFYGGDLWGIAEKVDYFEELGINVLYLTPIFLSDTNHKYDTIDYFRVDPQFGGKRAFLHLLRVLHERSMKLILDGVFNHVGSQHPWFKKAKKNDPEYVNRFFLYKDRHRSWFDVGSLPELNVEVEEVKEYILKVVEHYLKLGIDGWRLDCGHDLGPTVNLWINMKVKEFSAEKYLVSEIWTYPAGWDMVDGLMNYNFRNLVLSYVNGETDSIGFHLERAYRETKNIFGCWNMLDSHDTPRLATMVPDRDLRKLAVVLQFTYPGVPLVYYGTEIGLTGGEDPECRATMEWNREKWDVDLFEFYKKMIRLRRTDPGLRFGEFVLLNDSPLAFLRKAPHPLQNTIVVVNPGEEKVLVLSIPDGKIMNTTPLVDVFSGERFHVDGGVVKLPLLARSFRILKPEDLRVGKYRLYKRV

aglB

Cyclomaltodextrinase

472 amino acids

Is able to efficiently hydrolyze alpha-, beta-, and gamma-cyclomaltodextrins and linear maltooligosaccharides, to glucose and maltose, by decycling cyclodextrins and liberating glucose from the reducing end of the molecules. Shows a very weak activity on starch and pullulan. Cannot hydrolyze maltose or disaccharides with various types of alpha-links. Is not active against beta-glycosidic bonds of poly-, oligo-, and disaccharides.

Belongs to the glycosyl hydrolase 13 family.

55.112 kDa

MYPIPSWVYDSVVYQIFPDRFFIGKGKTVEDKKDLYLKRGGTIEKWGVPPRKLPGAQHVKVFYGGDLWGIAEKIDYLEELGVNAVYLTPIFLSDTNHKYDTIDYFKIDPQFGGKRAFVHLLKVLHSRNIKLILDGVFNHVGSQHPWFKKARKKDPEYVNRFFLYRDRHRSWFDVGSLPELNVEVEEVREYILKVVQHYLEVGVDGWRLDCGHDLGPLVNLWINMKVKEFSSEKYLVSEIWTYPAGWEMVDGLMNYNFRSLVLSYVNGETDSIGTELERAYRETKNIFGCWNMLDSHDTPRLATTVPVKDLRKLAIVLQFTYPGVPLVYYGTEIGLTGGEDPECRATMEWNREKWDMELFEFYKKMIRFRRTDPGLRFGEFILLKEKPLAFMRKAPHPLQDTIVVVNPEEEKNVVLSLPDGKIMNATPLFDIFTGEKFHVDGGVVKVPVGRRSFRILKPIDLRVGRYRLYKRV

aglB

Probable alpha-galactosidase B

455 amino acids

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.

Belongs to the glycosyl hydrolase 27 family.

50.42 kDa

MIEFLALITLISRANALMRPDGVGRLPALGWSSWNAHECDINATVILTAAAQVVKLGLKDLGYEYINIDDCWSIKTHRDPTTNRMIPDADRFPDGIASVASQIHELGLKVGIYSSAGETTCAGYPASLGYEDIDAETFAEWEIDYLKYDDCGVPDNWKDPYTFCVPDTANNAGPFPNGTCPSLPNPAPANYNWSTSPSAERFRRMLDALNTQDRTILYSLCNWGNAAVNTWGAEIGNSWRMSGDISPGRGEVGPDRTRIAVWERIAEITNEMSFLVREYAEFWGWPDADMLEVGNGEGGMTVAENRAHFALWAAMRSPLLIGTKLDTIRQEHLKILKNPTLLTFHQDPIINRPAYPYKWGYNADYTFDAAHPAEYWSGPSPALEGTLVVMLNSENVTSTRMAVWSEVPELQNQDQGDAFEVMDGWTGEDLGCIKEKYEVELDAHDAAVLVVGNAC

aglB

Probable alpha-galactosidase B

448 amino acids

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.

Belongs to the glycosyl hydrolase 27 family.

49.135 kDa

MSRFHLPLAAAVVLVSCLWSANALVRPDGVGKLPALGWNSWNAFGCDIDDAKIMTAAKEIVNLGLKDLGYEYINIDDCWSVKSGRDKTTKRIVPDPAKFPDGIAGVADRIHDLGLKVGIYSSAGLTTCAGYPASLGYEEIDAQTFAEWGIDYLKYDNCGVPSNWTDAYTFCVPDPGSASTNGTCPDNENPAPQGYDWSTSLTAQRHQRMRDALLGVEHTIFYSLCEWGQADVSAWGNATGNSWRMSGDITPSWDRIAAIANENSFLLNHVDFWGHSDPDMLEVGNGDLTLAENRAHFALWAAMKSPLIIGTALDGIDPAHLEILLNKYLIAFHQDPVIGRPAYPYKWGYSPDWTFDPAHPAEYWSGPSSTLDGTLVLMLNSEGSRQTRTAVWKEIPELKDALGRKGRRQTGFRVTDVWTGKDLGCVRDHYTVTLESHDVAALLVGKGC

aglB

Probable alpha-galactosidase B

447 amino acids

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.

Belongs to the glycosyl hydrolase 27 family.

49.202 kDa

MTTFFSLTTAAAVLTLARGSNALVRPGNVGKLPALGWNTWNAFGCDIDATKIMTAANEVVNLGLKDLGYEYINIDDCWSVKSGRDASTQRIIPDPDKFPDGISGVADQIHDLGLKIGIYSSAGLTTCAGYPASLGYEDIDAQTFAEWGIDYLKYDNCGVPSNWTDTYTYCVPDPGSKATNGTCPDNKNPAPAGYDWRTSLTAERYRRMRDALVSVDRTILYSLCEWGQANVNDWGNETGNSWRTTGDITPSWPRIAAIANENSFLMNHVDFWGYPDPDMLEVGNGNLTLAENRAHFALWAAMKSPLIIGTALDSISQDHLAILSNKILLKFHQDPVIGRPAQPYKWGYNPDWTFDPAHPAEYWSGASSVLGGTLVLMLNSEDTTQRRTAVWKEVPELKDVLGRQGKRRIGFRVTDVWTGKDLGCVRDHYSVELESHDVAALVVGRAC

aglB

Probable alpha-galactosidase B

443 amino acids

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.

Belongs to the glycosyl hydrolase 27 family.

48.827 kDa

MRWLLTSSALLVPAAALVRPDGVGRTPALGWNSWNAYSCDIDADKIVTAANEVVNLGLKDLGYEYINIDDCWSVKSGRNTTTKRIIPDPDKFPNGISGVADQVHALGLKLGIYSSAGLTTCAGYPASLGYEEIDAQSFAEWGIDYLKYDNCGVPTNLTDQYTYCVPDSTDGSNYPNGTCVNLTDAAPQGYDWATSTTAKRYQRMRDALLSVNRTILYSLCDWGQADVNAWGNATGNSWRMSGDITATWSRIAEIANENSFLMNYANFWGYPDPDMLEVGNGNLTLPENRAHFALWAMMKAPLIIGTPLDSIDTSHLTILSNKPLLTFHQDAVIGRPAYPYKWGYNPDWTFDPEHPAEYWSGPTSSGEVFVLMLNSEGEVKTRSAVWEEVPELKDRGTKKNSKEKKGFKVTDAWTGKDLGCVKDKYEVKLQAHDVAVLVVGGQC

aglB

Probable alpha-galactosidase B

443 amino acids

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.

Belongs to the glycosyl hydrolase 27 family.

48.784 kDa

MRWLLTSSALLVPAAALVRPDGVGLTPALGWNSWNAYSCDIDADKIVTAANEVVNLGLKDLGYEYINIDDCWSVKSGRNTTTKRIIPDPDKFPNGISGVADQVHALGLKLGIYSSAGLTTCAGYPASLGYEEIDAQSFAEWGIDYLKYDNCGVPTNLTDQYTYCVPDSTDGSNYPNGTCVNLTDAAPQGYDWATSTTAKRYQRMRDALLSVNRTILYSLCDWGQADVNAWGNATGNSWRMSGDITATWSRIAEIANENSFLMNYANFWGYPDPDMLEVGNGNLTLPENRAHFALWAMMKAPLIIGTPLDSIDTSHLTILSNKPLLTFHQDAVIGRPAYPYKWGYNPDWTFDPEHPAEYWSGPTSSGEVFVLMLNSEGEVKTRSAVWEEVPELKDRGTKKNSKEKKGFKVTDAWTGKDLGCVKDKYEVKLQAHDVAVLVVGGQC

aglB

Probable alpha-galactosidase B

442 amino acids

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.

Belongs to the glycosyl hydrolase 27 family.

48.493 kDa

MQRYISLSVSLSLLSGANALVRPDGVGRLPALGWNTWNAFGCDIDASKVLTAAEETINLGLKDAGYEYINIDDCWSVKSGRDPNTKRIIPDSAKFPDGISGVASKIHDLGLKVGIYSSAGTETCAGYPASLGYEKIDAESFAEWGIDYLKYDNCGVPTNWTDTYTHCVPDNSNGSKFPNGTCPDISNPAPTAYDWSSSNTAQRYNAMRDALLGVNRTILYSLCEWGQADVNTWGNGTGNSWRTTGDITPDWSRIVEIANENSFLMNYADFWGYPDPDMLEVGNGNLTLEENRAHFALWAAMKSPLIIGTALDSINEEHLAILKNKPLLSFHQDPVIGRPAYPYKWGYNPDWTFDPAHPAEYWSGPSSTLGGTLVLMFNSEDSAKHRTAVWSEIPELKDSAEKGSGYRVTDIWTGEDLGCVKDQYDVELQSHDIAALVVGESC

aglB

Probable alpha-galactosidase B

442 amino acids

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.

Belongs to the glycosyl hydrolase 27 family.

48.507 kDa

MQRYISLSVSLSLLSGANALVRPDGVGRLPALGWNTWNAFGCDIDASKVLTAAEETINLGLKDAGYEYINIDDCWSVKSGRDPNTKRIIPDSAKFPDGISGVASKIHDLGLKVGIYSSAGTETCAGYPASLGYEKIDAESFAEWGIDYLKYDNCGVPTNWTDTYTHCVPDNSNGSKFPNGTCPDISNPAPTAYDWSSSNTAQRYNAMRDALLGVNRTILYSLCEWGQADVNTWGNGTGNSWRTTGDITPDWSRIVEIANENSFLMNYADFWGYPDPDMLEVGNGNLTLEENRAHFALWAAMKSPLIIGTALDSINEEHLAILKNKPLLSFHQDPVIGRPAYPYKWGYNPDWTFDPAHPAEYWSGPSSTLGGTLVLMFNSEDSAKHRTAVWSEIPELKDSAEKGSGYRVTEIWTGEDLGCVKDQYDVELQSHDIAALVVGESC

aglB

6-phospho-alpha-glucosidase

440 amino acids

Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including maltose-6'-phosphate, isomaltose-6'-phosphate, maltitol-6-phosphate, trehalose-6-phosphate and the 6'-phosphorylated derivatives of the five linkage-isomeric alpha-D-glucosyl-D-fructoses: trehalulose-6'-phosphate, turanose-6'-phosphate, maltulose-6'-phosphate, leucrose-6'-phosphate, and palatinose-6'-phosphate. However, sucrose-6-phosphate is not a substrate for this enzyme.

Belongs to the glycosyl hydrolase 4 family.

49.255 kDa

MKKFSVVIAGGGSTFTPGIVLMLLANQDRFPLRSLKFYDNDGARQETIAEACKVILKEQAPEIEFSYTTDPQAAFTDVDFVMAHIRVGKYPMREQDEKIPLRHGVLGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPNAKILNICDMPIGIEGRMAQIVGLKDRKQMRVRYYGLNHFGWWTSIEDLDGNDLMPKLREYVAKYGYVPPSNDPHTEASWNDTFAKAKDVQALDPQTMPNTYLKYYLFPDYVVAHSNPERTRANEVMDHREKNVFSACRAIIAAGKSTAGDLEIDEHASYIVDLATAIAFNTQERMLLIVPNNGAIHNFDADAMVEIPCLVGHNGPEPLTVGDIPHFQKGLMSQQVAVEKLVVDAWEQRSYHKLWQAITLSKTVPSASVAKAILDDLIAANKDYWPELH

aglB

Probable alpha-galactosidase B

426 amino acids

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.

Belongs to the glycosyl hydrolase 27 family.

47.215 kDa

MSRSKTRQGKLPALGWNTWNAFGCDIDATKIMTAANEVVNLGLKDLGYEYINIDDCWSVKSGRDASTQRIIPDPDKFPDGISGVADQIHDLGLKIGIYSSAGLTTCAGYPASLGYEDIDAQTFAEWGIDYLKYDNCGVPSNWTDTYTYCVPDPGSKATNGTCPDNKNPAPAGYDWRTSLTAERYRRMRDALVSVDRTILYSLCEWGQANVNDWGNETGNSWRTTGDITPSWPRIAAIANENSFLMNHVDFWGYPDPDMLEVGNGNLTLAENRAHFALWAAMKSPLIIGTALDSISQDHLAILSNKILLKFHQDPVIGRPAQPYKWGYNPDWTFDPAHPAEYWSGASSVLGGTLVLMLNSEDTTQRRTAVWKEVPELKDVLGRQGKRRIGFRVTDVWTGKDLGCVRDHYSVELESHDVAALVVGRAC